摘要：Existing technologies used to detect monosodium urate(MSU)crystals for gout diagnosis are not ideal due to their low sensitivity and complexity of *** purpose of this study was to explore whether aggregation-induced emission luminogens(AIEgens)can be used for highly specific imaging of MSU crystals to assist in the diagnosis of ***,we developed a series of luminogens(i.e.,tetraphenyl ethylene(tpe)-NH_(2),tpe-2NH_(2),tpe-4NH_(2),tpe-COOH,tpe-2COOH,tpe-4COOH,and tpe-ketoalkyne),each of which was then evenly mixed with MSU ***,optimal fluorescence imaging of each of the luminogens was characterized by a confocal laser scanning microscope(CLSM).This approach was used for imaging standard samples of MSU,hydroxyapatite(HAP)crystals,and mixed samples with 1:1 mass ratio of MSU/*** also imaged samples from mouse models of acute gouty arthritis,HAP deposition disease,and comorbidities of ***,CLSM imaging results were compared with those of compensated polarized light microscopy,and we assessed the biosafety of tpe-ketoalkyne in the RAW264.7 cell ***,CLSM time series and three-dimensional imaging were performed on MSU crystal samples from human gouty synovial fluid and *** a promising candidate for MSU crystal labeling,tpe-ketoalkyne was found to detect MSU crystals accurately and rapidly in standard samples,animal samples,and human samples,and could precisely distinguish gout from HAP deposition *** work demonstrates that tpe-ketoalkyne is suitable for highly specific and timely imaging of MSU crystals in gouty arthritis and may facilitate future research on MSU crystal-related diseases.