摘要：Based on coral proxies we reconstructed the western Pacific warm pool sea surface temperature (SST) since 1644 AD. High-frequency reconstructions are based on eight high-pass filtered coral series and raw reconstructions are derived from eight unfiltered coral series, respectively. Validation and comparison with other SST/temperature series show that the reconstructed warm pool SST is highly reliable. The leading periods of warm pool SST are ～2.1, ～2.3, ～2.9, ～3.6, ～3.8, and 80.7-year during the last ～360 years. The warm pool SST exhibits some obvious long-term trends: an upward trend of 0.04°C per century for the period of 1644–1825, while a decreasing trend of 0.24°C per century for the period of 1826–1885, and then a remarkable warming trend of 0.28°C per century taking place between 1886 and 2006. Especially, the SST shows the strongest trend of 0.67°C increase per century during the last 50 years, a warming unprecedented since 1644 AD. On interannual timescale, the connections between ENSO and the warm pool SST are robust during the reconstruction period. There are significant correlations between the warm pool SST and summer precipitation of the Yellow River basin and Huaihe River basin; the correlation coefficients are ?0.44 in reconstruction period (1880–1949 AD) and ?0.46 in instrumental period (1950–2005 AD) respectively. This relationship is also found between flood-drought index and the warm pool SST during the past 360 years, and their correlation coefficients are ?0.20 in reconstruction period and ?0.46 in instrumental period respectively, significant at the 0.01 level. On interdecadal timescale, this connection is more robust, and the correlation coefficient of the low-pass filtered components is ?0.42 during the whole period (1644–2000 AD). When the warm pool is warmer than normal, the precipitation is usually below the normal in the Yellow River and Huaihe River basin. On the contrary, when the warm pool is colder than the normal, there may be more

摘要：A Rose for Emily is a short story written by William Faulkner, an American modernist novelist, in the first half of the 20th century. This paper tries to analyze the class structure and class discrimination in the Southern United States from the perspective of western Marxist literary criticism in the 19th to 20th centuries of the text, and further expand the thematic significance of the literary text.

摘要：In the worldwide context of globalization,translation has played an increasingly important role in the cultural exchanges between different cultures,nations,and *** globalization has brought China into the global village via the World Trade Organization,the demand for translated Chinese texts has been on the rise for both commercial and cultural ***,due to the radical differences between Chinese and West-

摘要：The field of Early China studies is sometimes faulted for being *** here for once is a book that addresses an issue of obvious relevance to our world:the roots of Chinese *** Feng plumbs the bronze inscriptions of the western Zhou period for information on govemment officeholders and their activities,and he presents a complex and largely plausible reconstruction of the operation of the royal administration.

摘要：Imagination of China in western movies presented by“exoticism”is no longer the dualism of“backward and traditional Oriental”but“advanced and modern western”.Skyfall released recently has shown on the one hand a“modern”contemporary China,on the other hand,a cultural concept of“isolated relics of traditional China and ruins of modern China”.In Indiana Jones and the Temple of Doom,China is imagined to be a mixed land of the new and old,the Oriental and western based on the comparison between China and India,which is considered to be primitive,traditional and ***:Impossible III presents three sides of Shanghai in a mean way:“skyscraper”,“old-style apartment”and“ancient town”.Here we could see,the images of China in western movies are comprehensive and complicated,conveying divergent cultural ***,increasing Chinese market constitutes a crucial reason why western movies intend to hide their political bias but adopt a flattery attitude to Chinese culture.

摘要：采用L9(34)正交设计法,测定了不同光圈、焦距、曝光时间下成像的条带和背景灰度值,探讨了光圈、焦距、曝光时间对NR2A的western Blot成像效果的影响.结果表明,光圈和曝光时间的影响具有显著性,NR2A的最佳成像条件是光圈为4mm、焦距为30mm、曝光时间为60s.

摘要：western Digital(西部数据）公司在激烈的市场竞争中立于不败得益于业界领先的技术背景和强大的制造工艺。该公司曾经为ULTRA ATA规范的制定和实施做出了巨大的贡献，而近年来整个存储工业磁盘记录密度的提升也与他们的不懈努力密不可分。有意思的是western Digital公司的产品很长时间以来一直坚持使用3张盘片组成整数GB容量（如100GB和200GB）的设计思路。

摘要：目的小鼠B7反义肽与小鼠CD40L胞外区融合蛋白(mB7AP-exCD40L)的分子模拟设计及其酵母表达体系的建立,研究其酵母表达产物的生物活性。方法分子模拟设计mB7AP-exCD40L;构建pPIC9K-mB7AP-exCD40L质粒并用电击法转化PichiapastorisGS115。westernblot鉴定融合蛋白的表达,混合淋巴细胞反应(MLR)研究mB7AP-exCD40L对淋巴细胞增殖的影响。结果构建的重组Pichiapastoris实现了mB7AP-exCD40L的分泌表达,表达蛋白质的相对分子质量约2.6×103,对MLR具有抑制作用。结论分子模拟设计的mB7AP-exCD40L在Pichiapastoris表达体系成功表达,为进一步研究mB7AP-exCD40L在抗移植排斥反应中的作用奠定了基础。

摘要：详细说明了如何通过对特定基因mRNA序列进行分析设计具有较高基因沉默效应的siRNA方法,并应用该方法针对小鼠早幼粒细胞白血病基因pml3设计相应的siRNA.通过RTPCR和westernBlot的方法,验证了其对pml3基因表达的抑制效果,实验证明通过我们提出的方法所设计的siRNA具有较高的基因沉默效应.

摘要：目的:初步建立子宫内膜癌雌激素受体亚型α(estrogenreceptorα, ERα)弱表达细胞模型,探讨ERα在子宫内膜癌发病机制中的作用,研究 17β 雌二醇(17β estradiol, E2 )及三苯氧胺(tamoxifen, TAM)在子宫内膜癌发病过程中与ERα的关系。方法:设计针对ERα的反义寡核苷酸 (antisenseoligodexyribonucleotides, AS ODN),转染入子宫内膜癌HEC 1B细胞,westernblot测定转染后细胞ERα的表达;检测转染反义寡核苷酸后E2 和TAM对子宫内膜癌细胞生长影响的变化。结果: (1)针对ERα翻译启动区设计的反义寡核苷酸可以选择性下调ERα的表达。(2)E2 和TAM可以促进子宫内膜癌细胞系HEC 1B的生长。转染ERα AS ODN后可抑制E2 对子宫内膜癌细胞HEC 1B的促生长作用,这种抑制作用以转染后的 24, 48及 72h尤为明显。转染ERα AS ODN可抑制TAM对子宫内膜癌细胞HEC 1B的促生长作用,这种抑制作用在转染后的 48h最为明显。结论: 初步建立了ERα弱表达的子宫内膜癌细胞模型。反义核酸技术可以作为一种有效的特异性下调子宫内膜癌细胞中ERα的实验手段。在HEC 1B细胞中,ERα抑制E2 和TAM的促进子宫内膜癌细胞生长的作用。