摘要：In assessing an insect-resistant genetically engineered （IRGE） crop before its commercialization, researchers normally use so-called ＂Tier-1 assays＂ as the initial step to determine the effects of the crop on non-target organisms. In these tests, the insecticidal proteins （IPs） produced by the IRGEs are added to the diets of test organisms in the laboratory. Test organisms in such assays can be directly exposed to much higher concentrations of the test IPs than they would encounter in the field. The results of Tier-1 assays are thus more conservative than those generated in studies in which the organisms are exposed to the IPs by feeding on IRGE plant tissue or in the case of predators or parasites, by feeding on invertebrate prey or hosts that have fed on IRGE plant tissue. In this report, we consider three important factors that must be considered in Tier-1 assays： （i） methods for delivery of the IP to the test organisms; （ii） the need for and selection of compounds used as positive controls; and （iii） methods for monitoring the concentration, stability and bioactivity of the IP during the assay. We also analyze the existing data from Tier-1 assays regarding the toxicity of Bt Cry proteins to non-target arthropod species. The data indicate that the widely used Bt proteins have no direct toxicity to non-target organisms

摘要：Timber bridges can provide an economical alternative to concrete and steel structures, particularly in rural areas where vehicle traffic is light to moderate. The wooden components of bridges have historically been preserved with either an oil type or waterborne preservative system to protect the wood from decay in order to maintain required performance standards for an extended period of time. The focus of this paper is to describe some of the key preservatives, research and case studies that support use of preserved wood, and some important steps to follow for the appropriate and safe use of preserved wood when the planned application will be in or over aquatic and wetland environments. A wealth of scientific information has been collected and analyzed that clearly suggests the use of preserved wood does not present a significant adverse effect on aquatic and wetland environments. This conclusion is based on two decades of empirical research and case study evaluating the environmental fate and effects of preserved wood, level of migration of contaminates into aquatic and marine environments, and the preserved wood environment. This is particularly true when risks are properly assessed on a project site, the appropriate preservative is selected and the wood is preserved to the Western Wood Preservers Institute＇s BMPs （best management practices）, along with properly installing and maintaining the preserved material. To assist with the assessment process, peer-reviewed risk assessment models for 11 commonly used preservatives have been developed that provide for streamlined data entry by users and allow for evaluation of a structure above and below water. A companion preliminary screening level assessment tool is also available. When these measures are properly utilized engineers, biologists and other responsible officials can be confident that the service life of the preserved wood components will more than likely meet the required performance standards in an enviro