摘要：Dear Editor,Clustered regularly interspaced short palindromic repeats associated protein(CRISPR/Cas)systems are convenient and versatile tools for genome *** by a guide RNA(gRNA),the Cas nuclease produces double-strand break(DSB)at the target site,generally resulting in small base insertion/deletion mutations by the non-homologous end joining(NHEJ)DNA repair.

摘要：One promising joining method for NiTi-SMA （shape memory alloy）-components is laser welding. This joining technology bears huge potential regarding process automation and mechanical properties as well as durability, especially within the field of small- and medium-sized actuators. However, there is still need for research due to unsolved issues influencing the microstructure and thus effecting mechanical properties as well as SMA-characteristics of these joints. Therefore, the purpose of this paper is the evaluation of quality parameters of NiTi-NiTi-wire-joints. For this purpose, design of experiments with a fractional factorial design is used for the investigation, because of its high potential to decrease experimental effort. This paper provides a basis for future research in the field of SMA-actuators and joining.