摘要：Maltose is a natural α-(1,4)-linked disaccharide with wide applications in food industries and microbial fermentation. However,maltose has scarcely been used for in vitro biosynthesis, possibly because its phosphorylation by maltose phosphorylase (MP)yields β-glucose 1-phosphate (β-G1P) that cannot be utilized by α-phosphoglucomutase (α-PGM) commonly found in in vitrosynthetic enzymatic biosystems previously constructed by our group. Herein, we designed an in vitro synthetic enzymaticreaction module comprised of MP, β-phosphoglucomutase (β-PGM), and polyphosphate glucokinase (PPGK) for thestoichiometric conversion of each maltose molecule to two glucose 6-phosphate (G6P) molecules. Based on this syntheticmodule, we further constructed two in vitro synthetic biosystems to produce bioelectricity and fructose 1,6-diphosphate (FDP),respectively. The 14-enzyme biobattery achieved a Faraday efficiency of 96.4% and a maximal power density of 0.6mW/cm^(2),whereas the 5-enzyme in vitro FDP-producing biosystem yielded 187.0mM FDP from 50 g/L (139mM) maltose by adopting afed-batch substrate feeding strategy. Our study not only suggests new application scenarios for maltose but also provides novelstrategies for the high-efficient production of bioelectricity and value-added biochemicals.

摘要：在本文，我们证明了：若群Ｇ满足Ｓｚ（２２ｍ＋１）≤Ｇ≤ＡｕｔＳｚ（２２ｍ＋１）ｍ≥１，且Ｇ作用在２－（ｖ．ｋ．１）设计上是线本原的，则Ｇ也是点本原的．

摘要：A novel kind of multidimension symbol/sequence trellis coded modulation (TCM) based on TCM of lower coding rate has been constructed. The redundancy of this new kind of TCM is provided not only by the modulated symbol sequence but also by the PN sequences for spreading spectrum as well. The performance of direct sequence spread spectrum multiple access (DS/SSMA) system with this symbol/sequence TCM over flat Rayleigh fading channel has been investigated by theoretical analysis and numerical simulation. The results obtained in this research demonstrate the performance improvements of this system in comparison with that of the DS/SSMA system with conventional TCM under the same conditions.