摘要：This work develops a heuristic method for the design of batch water-using networks of multiple contaminants with regeneration unit based on the concepts of concentration potential. A water-using network involving regeneration unit can be formed by adding the regenerated stream(s) into the network involving reuse only. In the design procedure of the network operated in a single batch mode, time is take n as the primary factor a nd con centration potentials as the secondary one. For the networks operated in a repeated mode, the design procedure is similar to that for continuous processes, besides designing the storage tanks with the rules proposed. Continuous regeneration unit is selected in this work. With the proposed method, the network structure corresponding to the minimum freshwater consumption can be obtained. It is shown that the method proposed in this article is simple, effective and has clear engineering meaning.

摘要：A model of the irreversible regenerative Brayton refrigeration cycle working with paramagnetic materials is established,in which the regeneration problem in two constant-magnetic field processes and the irreversibility in two adiabatic processes are considered *** for the COP,cooling rate,power input,theminimum ratio of the two magnetic fields,etc.,are *** is found that the influence of the irreversibility and the regeneration on the main performance parameters of the magnetic Brayton refrigerator is *** is important that we have obtained several optimal criteria,which may provide some theoretical basis for the optimal design and operation of the Brayton *** results obtained in the paper can provide some new theoretical information for the optimal design and performance improvement of real Brayton refrigerators.

摘要：Bone tissue engineering (BTE) is a rapidly developing strategy for repairing critical-sized bone defects to address the unmet need for bone augmentation and skeletal repair. Effective therapies for bone regeneration primarily require the coordinated combination of innovative scaffolds, seed cells, and biological factors. However, current techniques in bone tissue engineering have not yet reached valid translation into clinical applications because of several limitations, such as weaker osteogenic differentiation, inadequate vascularization of scaffolds, and inefficient growth factor delivery. Therefore, further standardized protocols and innovative measures are required to overcome these shortcomings and facilitate the clinical application of these techniques to enhance bone regeneration. Given the deficiency of comprehensive studies in the development in BTE, our review systematically introduces the new types of biomimetic and bifunctional scaffolds. We describe the cell sources, biology of seed cells, growth factors, vascular development, and the interactions of relevant molecules. Furthermore, we discuss the challenges and perspectives that may propel the direction of future clinical delivery in bone regeneration.

摘要：Hydrogel encapsulation has been widely utilized in the study of fundamental cellular mechanisms and has been shown to provide a better representation of the complex in vivo microenvironment in natural biological conditions of mammalian *** this review,we provide a background into the adoption of hydrogel encapsulation methods in the study of mammalian cells,highlight some key findings that may aid with the adoption of similar methods for the study of plant cells,including the potential challenges and considerations,and discuss key findings of studies that have utilized these methods in plant sciences.

摘要：A study was carried out to evaluate the effect of shelterwood cutting methods on stand structure and regeneration density. Data were collected from a northern hardwood forest stands in Iran with Fagus orientalis Lipsky as dominant species, with/without shelterwood cutting operation. Results clearly demonstrate that the management of Fagus orientalis Lipsky with shelterwood cutting system affected the frequency and diversity of the understory herbaceous species. The frequency of Viola silvestris Lam., Asperula odorata L., Carex spp. and Rubus hyr-canus Juz increased significantly after shelterwood cutting. The DBH （diameter at breast height） of commercial species in control stands （57.50±2.15 cm） was greater than that in treated stands （50.67±1.88 cm）, whereas the total height of trees was similar between treated and control plots （21±0.5 m）. The number of Parrotia persica seedlings increased by 13.2% from 1995 to 2005 whereas the number of Fagus orientalis and Carpinus betulus seedlings significantly decreased from 1995 to 2005. In conclusion, it confirms that instead of shelterwood cutting method other silvicultural practices such as selection cutting method should be applied for the mountainous beech stands of Hyrcanian forests.

摘要：Under the background that urban development has been renovated constantly, the paper has analyzed cultural circulation system and recycling effect in urban renovation, and emphasized that traditional culture should be extended with industrial heritage as the carrier, so as to transform post- industrial negative heritage into modern civilization adapting to modern life. By taking Beijing "789" for example, its development process and situation have been introduced. Based on the spirit of place, the practical significance of cultural creative park and reuse of industrial heritage have been illustrated. It considers that cultural creation can not only extend cultural context, but also help to find new inspiration in industrial heritage, transforming the function of negative landscape in urban renovation in the form of "catalysis", improving its economic value, social cultural value and cultural effective circulation value.

摘要：[Objective] This study aimed to explore the protoplast preparation and re- generation conditions of Tremella aurantialba. [Method] Optimal combination of five factors affecting the protoplast preparation and regeneration of Tremella aurantialba was selected by using orthogonal experiment, including enzyme system, culture age, enzymolysis temperature, enzymolysis duration and osmotic stabilizer. [Result] The results showed that there were three release modes of Tremella aurantialba proto- plasts： release from top in the early period of enzymolysis, release from the side and release in situ. The optimal protoplast preparation condition was selecting mycelium cultured in liquid medium for 3 d, for enzymolysis in mixed enzyme solu- tion containing 1% of cellulase ＋1% of snailase ＋1% of lywallzyme at 36 ℃; for 4 h, with 0.6 mol/L sucrose as osmotic stabilizer, and the obtained preparation rate had reached 1.76 ×10^7 protoplasts/ml. The optimal regeneration condition was using mycelium cultured in liquid medium for 5 d, for enzymolysis in enzyme solution con- taining 1.5% of lywallzyme at 33 ℃ for 3 h, with 0.6 mol/L sucrose as osmotic sta- bilizer, and the regeneration rate had reached 0.22%. [Conclusion] This study provid- ed valuable support for protoplast fusion, ultraviolet mutation breeding and genetic engineering research.

摘要：Recently,tissue engineering has developed into a powerful tool for repairing and reconstructing damaged tissues and *** engineering scaffolds play a vital role in tissue engineering,as they not only provide structural support for targeted cells but also serve as templates that guide tissue regeneration and control the tissue *** the past few years,owing to unique physicochemical properties and excellent biocompatibility,various types of two-dimensional(2D)nanomaterials have been developed as candidates for the construction of tissue engineering scaffolds,enabling remarkable achievements in bone repair,wound healing,neural regeneration,and cardiac tissue *** efforts have significantly advanced the development of tissue *** this review,we summarize the latest advancements in the application of 2D nanomaterials in tissue ***,each typical 2D nanomaterial is introduced briefly,followed by a detailed description of its applications in tissue ***,the existing challenges and prospects for the future of the application of 2D nanomaterials in tissue engineering are discussed.

摘要：Polycaprolactone-carboxymethyl cellulose composites have been obtained and used to print porous structures by material *** materials used contained 0,2 and 5% w/w of the carboxymethyl cellulose *** structures have been analyzed in terms of their morphology (including the evaluation of their porosity),mechanical properties under compression load and cell *** affinity has been evaluated by culturing sheep mesenchymal stem cells and analyzing their viability by the Alamar Blue(R)assay at days 1,3,6 and *** results show that composites samples have similar values of porosity and apparent density than pure polycaprolactone ***,samples containing 5%w/w of carboxymethyl cellulose have micropores on the filaments due to a hindered deposition *** characteristic affects the mechanical properties of the structures,so these ones have a mean compression modulus significantly lower than pure polycaprolactone ***, the samples containing 2%w/w of carboxymethyl cellulose show no significant difference with the pure polycaprolactone ones in terms of their mechanical ***,the presence of 2%w/w of additive improves cell proliferation on the surface of the porous *** complementary information,the flow properties of the composite materials were studied and the power law equations at 210℃ obtained,as this temperature was the 3D printing *** equations can be useful for simulation and designing purposes of other manufacturing processes.

摘要：Zeolite catalysts,such as H-mordenite(H-MOR),are readily deactivated by coke deposition in carbonylation *** modification of H-MOR can improve its stability but can lead to an undesirable loss in catalytic ***,we report the intrinsic impact of the pyridine adsorption behavior on H-MOR and the spacial hindrance of the zeolite frameworks on dimethyl ether(DME)carbonylation at a molecular *** discovered that acid sites at O2 positions,located on common walls of eight-membered ring(8-MR)side pockets and 12-MR channels,were active in DME carbonylation,but were unfortunately poisoned during pyridine *** functional theory calculations revealed that the pyridine-poisoned acid sites at the O2 positions could be easily regenerated due to the spacial hindrance of the zeolite ***,they can be facilely regenerated by proper thermal treatment,which induces 60%promotion in the catalytic activity along with a high *** findings demonstrate the determining role of O2 positions in H-MOR for DME carbonylation and provide a new avenue for the rational design of other efficient zeolite-relevant catalytic systems.