摘要：Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical *** this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the industrial workhorse of Saccharomyces ***,we rewired the yeast central metabolism by optimizing non-oxidative glycolysis pathway for an improved acetyl-CoA ***,we restricted the consumption of farnesyl pyrophosphate(FPP)by the down-regulation of squalene synthase using the PEST ***,we further explored the human lipid binding/transfer protein saposin B(hSapB)-mediated metabolic sink for an enhanced storage of lipophilic ***,the copper-induced GAL expression system was engineered to function in the yeast-peptone-dextrose medium for an increased biomass *** combining the abovementioned strategies,the final engineered yeast produced 166.79±10.43 mg/Ⅰβ-carotene in shake flasks,which was nearly 5-fold improvement of the parental carotenoid-producing ***,we envision that multidimensional strategies reported here might be applicable to other hosts for the future industrial development of carotenoid synthesis from renewable feedstocks.