摘要：Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain ***,we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle(ABP)***,we concurrently printed scaffolds with polycaprolactone,ABPs,and allo-BMSCs for appropriate support,providing bioactive factors and seed cells to promote *** vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic *** vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted *** experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells,chondrocytes,and *** implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect,promoting bone *** study contributes to our understanding of allo-BMSCs,providing information relevant to their future application.

摘要：Bone tissue engineering (BTE) is a rapidly developing strategy for repairing critical-sized bone defects to address the unmet need for bone augmentation and skeletal repair. Effective therapies for bone regeneration primarily require the coordinated combination of innovative scaffolds, seed cells, and biological factors. However, current techniques in bone tissue engineering have not yet reached valid translation into clinical applications because of several limitations, such as weaker osteogenic differentiation, inadequate vascularization of scaffolds, and inefficient growth factor delivery. Therefore, further standardized protocols and innovative measures are required to overcome these shortcomings and facilitate the clinical application of these techniques to enhance bone regeneration. Given the deficiency of comprehensive studies in the development in BTE, our review systematically introduces the new types of biomimetic and bifunctional scaffolds. We describe the cell sources, biology of seed cells, growth factors, vascular development, and the interactions of relevant molecules. Furthermore, we discuss the challenges and perspectives that may propel the direction of future clinical delivery in bone regeneration.

摘要：Arrhythmogenic right ventricular cardiomyopathy(ARVC)is a progressive disease characterized by adipose and fibrous replacement of the *** elevated testosterone levels have been implicated in the pathological process of ARVC,its exact contribution to cardiac fibrosis in ARVC remains *** this study,we analyzed the potential contribution of gender-based differences on the distribution of the low-voltage area in an ARVC cohort undergoing an electrophysiological study,which was indicated by feature ***,we established engineered cardiac spheroid models in vitro using patient-specific induced pluripotent stem cell(iPSC)-derived cardiomyocytes(iPSC-CMs)and iPSC-derived cardiac fibroblasts(icFBs).We elucidated the pathogenicity of abnormal splicing in the plakophilin-2(PKP2)gene caused by an intronic ***,pathogenic validation of the desmoglein-2(DSG2)point mutation further confirms the reliability of the ***,testosterone exacerbated the DNA damage in the mutated cardiomyocytes and further activated myofibroblasts in a chain *** conclusion,we designed and constructed an in vitro three-dimensionally-engineered cardiac spheroid model of ARVC based on clinical findings and provided direct evidence of the fibrotic role of testosterone in ARVC.

摘要：Pilot biomechanical design of biomaterials for artificial nucleus prosthesiswas carried out based on the 3D finite-element method. Two 3D models of lumbar intervertebral discrespectively with a real human nucleus and with the nucleus removed were developed and validatedusing published experimental and clinical data. Then the models with a stainless steel nucleusprosthesis implanted and with polymer nucleus prostheses of various properties implanted were usedfor the 3D finite-element biomechanical analysis. All the above simulation and analysis were carriedout for the L4/L5 disc under a human worst--daily compression load of 2000 N. The results show thatthe polymer materials with Young's modulus of elasticity E = 0.1-100 MPa and Poisson's ratio v=0.35-0.5 are suitable to produce artificial nucleus prosthesis in view of biomechanicalconsideration.

摘要：Micron/nano scale topographic modification has been a significant focus of interest in current titanium(Ti)surface ***,the influence of micron/nano structured surface on cell or bacterium behavior on the Ti implant has rarely been systematically ***,except for popular microgrooves,little work has been carried out on the reaction of cells to the bionic *** this study,several micro-pillars mimicking cell morphology were prepared on Ti surfaces by lithography and contact printing(ICP)method,and they were further decorated with nanotube arrays by anodization *** surface modifications remarkablly increased the surface roughness of pristine Ti surface from 91.17 nm±5.57 nm to be more than 1000 nm,and reduced their water contact angles from 68.3°±0.7°to be 16.9°±2.4°.Then,the effects of these hierarchical micron/nano scale patterns on the behaviors of MG63 osteoblasts,L929 fibroblasts,SCC epithelial cells and *** were studied,aiming to evaluate their performance in osseointegration,gingival epithelial sealing and antibacterial *** an innovative scoring strategy,our findings showed that square micro-pillars with 6μm width and 2μm height combined with 85 nm diameter nanotubes was suitable for implant neck design,while square micro-pillars with 3μm width and 3.6μm height combined with 55 nm diameter nanotubes was the best for implant body *** study reveals the synergistic effect of the hierarchical micron/nano scale patterns on MG63 osteoblasts,L929 fibroblasts,SCC epithelial cells and *** *** provides insight into the design of biomedical implant surfaces.

摘要：Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.

摘要：The current goal is to create a set of portable terminals with GPS/BD2 dual-mode satellite positioning, vital signs monitoring and wireless transmission functions. The terminal depends on an ARM processor to collect and combine data related to vital signs and GPS/BD2 location information, and sends the message to headquarters through the military CDMA network. It integrates multiple functions as a whole. The satellite positioning and wireless transmission capabilities are integrated into the motherboard, and the vital signs sensors used in the form of belts communicate with the board through Bluetooth. It can be adjusted according to the headquarters' instructions. This kind of device is of great practical significance for operations during disaster relief, search and rescue of the wounded in wartime, non-war military operations and other special circumstances.

摘要：Thank you very much for reviewing the case report carefully and proposing meaningful comments on evaluating angiosome of the supraclavicular *** view of your opinions and based on our clinical experience,we hereby reply as follows:*** safe length of the vascular pedicle of the flap。

摘要：AIM： To present the rationale, design, methodology, and the baseline data of the beijing Desheng Diabetic Eye Study（BDDES）, and to determine the prevalence of diabetic retinopathy（DR） and possible risk factors in patients with type 2 diabetes mellitus（T2DM） in an urban community of beijing, ***： Community-based prospective cohort study of persons diagnosed with T2DM aged 30 y or older. The main variables of interest are the presence and progression of DR as determined by the standardized ETDRS grading of seven fields fundus photographs. The presence and severity of DR were analyzed for possible correlations to non-genetic and genetic ***： A total of 1438 participants with data available for analysis, the prevalence of any DR was 35.4%. The prevalence of mild non-proliferative diabetic retinopathy（NPDR）, moderate NPDR, severe NPDR, and proliferative diabetic retinopathy was 27.7%, 2.6%, 0.5% and 4.5%, respectively. By multiple logistic regression analysis, risk factors for the presence of any DR included male（P=0.031）, lower income level（P=0.011）, lower education background（P=0.022）, longer duration of diabetes（P=0.001）, younger age at diabetic onset（P=0.001）, higher systolic blood pressure（P=0.007）, higher glycosylated hemoglobin A1 c levels（P=0.001）, high albuminuria（P=0.03）, and use of insulin（P〈0.001）. For vision-threatening DR, four factors were significant： younger age at diabetic onset（P〈0.001）,higher systolic blood pressure（P=0.042）, high albuminuria（P〈0.001）, and use of insulin（P〈0.001）. CONCLUSION： The BDDES is the first large-scale ongoing cohort study of a Chinese urban population of persons with type 2 diabetes. Using standardized grading system comparable to large cohort studies from western populations, our baseline data shows that the prevalence of DR and major risk factors in this Chinese ethnic population are comparable to that found in the western population studies.

摘要：The coronary artery calcification score and pericardial fat volume have recently been reported to be strongly associated with the severity and presence of coronary atherosclerosis. However, no studies have explored the outcome of phased progression of atherosclerosis by non-contrast computed tomography in asymptomatic people in China. The population-based cohort study in outcome of phased progression of atherosclerosis in China （PERSUADE）, an observational, longitudinal and prospective cohort study in a target population of healthy sub- jects based in Jidong Oilfield （China）, prospectively analyzes the outcome of phased progression of atherosclerosis by non-contrast computed tomography in healthy population. The results of this study are expected to be of value for utilizing noninvasive imaging combine with tradi- tional cardiovascular risk factors to create a risk stratification and find pertinent biomarkers associated with the outcome of phased progres- sion of atherosclerosis in healthy people, thereby could help to establish a more personalized treatment of clinical practice.