摘要：To the editor： In the recent article of Fang and colleagues1 ssessing prophylactic efficacy of a single-dose of dexamethasone before anesthesia induction on postoperative nausea and vomiting （PONV） in patients undergoing microvascular decompression for facial spasm, they showed that dexamethasone did not prevent PONV. Actually, meta-analyses of double-blind randomized controlled trials comparing dexamethasone with controls indicate that this antiemetic is effective in the prophylaxis of postoperative vomiting and reduced use of rescue antiemetics.2 In our views, some limitations of this study design may have made interpretation of their conclusions questionable.

摘要：Rapid and sensitive detection of various analytes is in high *** from its application in genome editing,CRISPR-Cas also shows promises in nucleic acid detection *** further exploit the potential of CRISPR-Cas for detection of diverse analytes,we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12 *** demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background,i.e.,the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 lmol/L,respectively,highlighting the advantages of simplicity,sensitivity,short detection time,and low cost compared with the state-of-the-art biosensing ***,this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.

摘要：Objective:To optimize the extraction and purification technologies of Gekko sulfated glycopeptide based on the content of glycopeptide,removal ratio of proteins,and anticancer ***:Different extraction methods,namely,water extraction,ultrasonic extraction,enzymatic hydrolysis-water extraction,and enzymatic hydrolysis-ultrasonic extraction were considered to determine the best extraction *** factor and orthogonal experiments were performed to determine the optimum extracting ***,enzymatic hydrolysis-Sevage,trichloroacetic acid(TCA),TCA-Sevage and enzymatic hydrolysis-TCA methods were tested to determine the best deproteinization *** glycopeptide content and protein removal ratio were analyzed by the phenol-sulfuric acid and Coomassie Brilliant Blue ***:Gekko sulfated glycopeptide obtained by water extraction could inhibit the proliferation of HepG2 and SKBR3,as well as promote that of *** glycopeptide content was 4.049%in the optimal extracting condition of a triple decoction extraction for 1 hour each time with a material to solvent ratio of 1:*** enzymatic hydrolysis-TCA method was found to be the optimal deproteinization method,with a protein removal ratio of 50.46%,glycopeptide content of 14.27%,and inhibitory ratio on human HepG2 cells of 49.06%.Conclusion:This extraction and purification technique for Gekko sulfated glycopeptide is reasonable,feasible,and provides a scientific basis for industrial production.

摘要：目的探讨维生素D受体(VDR)基因TaqⅠ和FokⅠ位点多态性与家庭乙型肝炎病毒表面抗原(HBsAg)阳性者聚集性之间的关系。方法采用以人群为基础的病例对照家系设计,征集27个病例家系成员288名和27个对照家系成员230名,分析VDR基因多态性;应用聚合酶链反应—限制性片段长度多态性方法对VDR基因TaqⅠ和FokⅠ基因型进行测定。结果VDR-TaqⅠ的TT基因型的频率在病例家系显著高于对照家系(P<0·05),VDR-FokⅠ的CC基因型的频率在病例家系显著高于对照家系(P<0·05);Taq IT-Fok IC单体型在病例家系出现的频率显著高于对照家系(OR=1·67,P<0·05),而Taq IC-Fok IT单体型在病例家系的分布频率显著低于对照家系(OR=0·24,P<0·05);用感染过HBV的家系血源亲属进行分析,结果相近。结论VDR基因多态性可能是影响家庭HBsAg阳性者聚集的重要遗传因素。

摘要：Brain growth charts and age-normed brain templates are essential resources for researchers to eventually contribute to the care of individuals with atypical developmental *** present work generates age-normed brain templates for children and adolescents at one-year intervals and the corresponding growth charts to investigate the influences of age and ethnicity using a common pediatric neuroimaging *** accelerated longitudinal cohorts with the identical experimental design were implemented in the United States and *** magnetic resonance imaging(MRI)of typically developing school-age children(TDC)was obtained up to three times at nominal intervals of1.25 *** protocol generated and compared population-and age-specific brain templates and growth charts,respectively.A total of 674 Chinese pediatric MRI scans were obtained from 457 Chinese TDC and 190 American pediatric MRI scans were obtained from 133 American ***-and age-specific brain templates were used to quantify warp cost,the differences between individual brains and brain *** growth charts for labeled brain network areas were *** analyses of cost functions supported the necessity of age-specific and ethnicitymatched brain templates,which was confirmed by growth chart *** analyses revealed volumetric growth differences between the two ethnicities primarily in lateral frontal and parietal areas,regions which are most variable across individuals in regard to their structure and ***-and ethnicity-specific brain templates facilitate establishing unbiased pediatric brain growth charts,indicating the necessity of the brain charts and brain templates generated in *** templates and growth charts as well as related codes have been made freely available to the public for open neuroscience(https://***/zuoxinian/CCS/tree/master/H3/Growth Charts).

摘要：The global outbreak of coronavirus disease 19(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2),has raised significant global *** a rapid,efficient,sensitive,and accurate point-of-care detection method is imperative for curbing SARS-Co V-2 ***,we screened a sequence,designed a set of highly sensitive loopmediated isothermal amplification primers(LAMP)and g RNA,and developed a user-friendly detection platform combining CRISPRCas12a and RT-LAMP technology to specifically detect SARS-Co V-2 and its 5 *** analysis and Cas12a-g RNA identification ensured sequence specificity,allowing us to identify SARS-Co V-2 *** developed a method for the detection of SARSCoV-2 using these primers in combination with LAMP amplification and CRISPR-Cas12a *** method is designed to detect SARS-CoV-2(NC_045512),Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),Delta(B.1.617.2)and Omicron(B.1.1.529).Additionally,it can differentiate SARS-CoV-2 from other *** analysis can be conducted by measuring fluorescence values,while qualitative analysis can be performed by observing fluorescence color point-of-care diagnosis changes with the naked *** results suggest that a set of novel sensitive LAMP primers and g RNA have been obtained to detect the extensive variants,and the RT-LAMPCRISPR-Cas12a platform significantly facilitates point-of-care diagnosis,thereby halting the spread of SARS-Co V-2,thus contributing to COVID-19 prevention and control.

摘要：Background： Despite the rapid growth in the incidence of acute myocardial infarction （AMI） in China, there is limited information about patients＇ experiences after AMI hospitalization, especially on long-tern1 adverse events and patient-reported outcomes （PROs）. Methods： The China Patient-centered Evaluative Assessment of Cardiac Events （PEACE）-Prospective AMI Study will enroll 4000 consecutive AM I patients from 53 diverse hospitals across China and follow them longitudinally for 12 months to docunlent their treatment, recovery, and outcomes. Details of patients＇ medical history, treatment, and in-hospital outcomes are abstracted from medical charts. Comprehensive baseline interviews are being conducted to characterize patient demographics, risk factors, presentation, and healthcare utilization. As part of these interviews, validated instruments are administered to measure PROs, including quality of life, symptoms, mood, cognition, and sexual activity. Follow-up interviews, measuring PROs, medication adherence, risk factor control, and collecting hospitalization events are conducted at 1, 6, and 12 months after discharge. Supporting documents for potential outcomes are collected for adjudication by clinicians at the National Coordinating Center. Blood and urine samples are also obtained at baseline, 1 - and 12-month follow-up. In addition, we are conducting a survey of participating hospitals to characterize their organizational characteristics. Conclusion： The China PEACE-Prospective AMI study will be uniquely positioned to generate new information regarding patient＇s experiences and outcomes alter AMI in China and seiwe as a foundation for quality improveinent activities.

摘要：Two total synthetic routes for the preparation of myricetin were designed and *** was validated that route B presented an efficient approach to synthesizing myricetin,starting from commercially available and inexpensive *** was synthesized with an overall yield of 60%across three steps without the need of column chromatography *** successful preparation of myricetin on a 25-g scale underscored the potential of this approach.