摘要：Arrhythmogenic right ventricular cardiomyopathy(ARVC)is a progressive disease characterized by adipose and fibrous replacement of the *** elevated testosterone levels have been implicated in the pathological process of ARVC,its exact contribution to cardiac fibrosis in ARVC remains *** this study,we analyzed the potential contribution of gender-based differences on the distribution of the low-voltage area in an ARVC cohort undergoing an electrophysiological study,which was indicated by feature ***,we established engineered cardiac spheroid models in vitro using patient-specific induced pluripotent stem cell(iPSC)-derived cardiomyocytes(iPSC-CMs)and iPSC-derived cardiac fibroblasts(icFBs).We elucidated the pathogenicity of abnormal splicing in the plakophilin-2(PKP2)gene caused by an intronic ***,pathogenic validation of the desmoglein-2(DSG2)point mutation further confirms the reliability of the ***,testosterone exacerbated the DNA damage in the mutated cardiomyocytes and further activated myofibroblasts in a chain *** conclusion,we designed and constructed an in vitro three-dimensionally-engineered cardiac spheroid model of ARVC based on clinical findings and provided direct evidence of the fibrotic role of testosterone in ARVC.

摘要：In gamma camera and single-photon emission computerized tomography, the collimator removes most photons. Here, a gamma camera without collimator utilizes a specific arrangement of detectors. Instead of bending beams(like a lens) or directing beams(by parallel hole collimator), changes are created in detectors' field of view(FOV), so that each detector's FOV looks different from others. Simulation proved this theory, with 98 detectors(2 cm 9 1.41 cm) arranged in a zigzag manner for Monte Carlo simulation. A radioactive source with energy of140 ke V was situated on the detectors' faces. Sixty projections, each 3(0 –179) apart, were simulated by Monte Carlo N-Particle(MCNP) 4C code, rotating detectors around a radioactive point. The band containing the radioactive source is clearly visible in each *** obtained after simulation in different projections were reconstructed, and point source location emerged correctly. Simulation of gamma camera with zigzag arrangement of detectors and MCNP-4C code demonstrated that one could string the space and determine radioactive source by image reconstruction without using collimators, solely through these special detectors' distribution.

摘要：Biodegradable scaffolds are essential parts in hard tissue engineering. A highly porous magnesium-zinc （Mg-Zn 4 wt.%） scaffold with different Mg-Zn powder to liquid media ratios （50 wt.%, 70 wt.% and 90 wt.%） and different concentrations of ethanol （0 vol.%, 10 vol.%, 20 vol.% and 40 vol.%） were prepared through modified replica method. The mechanical properties were assessed through compression test and the structures of scaffolds were examined by Scanning Electron Microscope （SEM）. Results show that, the increase in Mg-Zn powder to liquid media ratio （50 wt.% to 90 wt.%） in ethanol free slurry, increases the thickness of struts （37 lam to 74 lam） and the plateau stress （0.5 MPa to 1.4 MPa）. The results obtained from X-ray Diffractometry （XRD） and compression test indicate that consuming ethanol in liquid media of replica, results in higher plateau stress by 46% due to less Mg-water reaction and no formation of Mg（OH）2 in the scaffold. The results of porosity measurement indicate that water-ethanol mixture composition and different solid fractions have no significant effects on true and apparent porosities of the fabricated scaffolds.

摘要：A series of novel arylpiperazine derivatives was synthesized. The in vitro cytotoxic activities of all synthesized compounds against three human prostate cancer cell lines（PC-3, LNCa P, and DU145） were evaluated by a CCK-8 assay. Compounds 8, 10, 13, 17 and 20 exhibited strong cytotoxic activities against the tested cancer cell lines（IC_（50）〈3 μmol/L）. In addition, these compounds exhibited weak cytotoxic effects on human epithelial prostate normal cells WPMY-1. The structure-activity relationship（SAR） of these arylpiperazine derivatives was also discussed based on the obtained experimental data.

摘要：One of the most important aims of this study is to improve the core of the current VVER reactors to achieve more burn-up(or more cycle length)and more intrinsic *** is an independent study on the Russian new proposed FAs,called TVS-2M,which would be applied for the future advanced *** important aspects of neutronics as well as thermal hydraulics investigations(and analysis)of the new type of Fas are conducted,and results are compared with the standards PWR *** TVS-2M FA contains gadolinium-oxide which is mixed with UO_(2)(for different Gd densities and U-235 enrichments which are given herein),but the core does not contain *** new type TVS-2M Fas are modeled by the SARCS software package to find the PMAXS format for three states of CZP and HZP as well as HFP,and then the whole core is simulated by the PARCS code to investigate transient *** addition,the WIMS-D5 code is suggested for steady core modeling including TVS-2M FAs and/or TVS *** neutronics aspects such as the first cycle length(first cycle burn up in terms of MWthd/kgU),the critical concentration of boric acid at the BOC as well as the cycle length,the axial,and radial power peaking factors,differential and integral worthy of the most reactive CPS-CRs,reactivity coefficients of the fuel,moderator,boric acid,and the under-moderation estimation of the core are conducted and benchmarked with the PWR ***,the burn-up calculations indicate that the 45.6 d increase of the first cycle length(which corresponds to 1.18 MWthd/kgU increase of burnup)is the best improving aim of the new FA type called ***,thermal-hydraulics core design criteria such as MDNBR(based on W3 correlation)and the maximum of fuel and clad temperatures(radially and axially),are investigated,and discussed based on the CDBL.

摘要：Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells(ECs)and are useful substrates for wound *** present study investigates the feasibility of covalent conjugation of taurine(Tau)on a gelatin-based *** hydrogel is expected to maintain positive charged growth factors such as basic fibroblast growth factor(bFGF)and vascular endothelial growth factors(VEGFs)near ECs within the hydrogel *** gelatin was conjugated with hydroxyl phenol(Ph)and Tau moieties,and in following that Ph residues were crosslinked through a horseradish peroxidase-catalyzed *** migration characteristics of ECs were analyzed by scratch migration assay and microparticle-based cell migration *** morphology and amounts of angiopoietin 1(Ang 1),bFGF,and VEGF proteins were evaluated for encapsulated *** potential of synthesized hydrogels in wound healing was assessed by the percentage of reduction from the original wound size and histopathological analyses of rat *** incorporated Tau molecules within the hydrogel remained stable through covalent bonds during *** extended incubation,the gelatin-based hydrogel conjugated with Tau improved the migration distance and number of existing migrated *** Tau within the gelatin-based hydrogel induced high motility of ECs,accompanied by robust cytoskeleton extension and a cell subpopulation that expressed CD44 and CD31 receptors as well as enhancement of Ang 1,bFGF,and *** found that injectable Gel-Ph-Tau effectively improves wound-healing parameters.

摘要：The development of natural biomaterials applied for hard tissue repair and regeneration is of great importance,especially in societies with a large elderly ***-assembled peptide hydrogels are a new generation of biomaterials that provide excellent biocompatibility,tunable mechanical stability,injectability,trigger capability,lack of immunogenic reactions,and the ability to load cells and active pharmaceutical agents for tissue ***-based hydrogels are ideal templates for the deposition of hydroxyapatite crystals,which can mimic the extracellular ***,peptide-based hydrogels enhance hard tissue repair and regeneration compared to conventional *** review presents three major self-assembled peptide hydrogels with potential application for bone and dental tissue regeneration,including ionic self-complementary peptides,amphiphilic(surfactant-like)peptides,and triple-helix(collagen-like)*** attention is given to the main bioactive peptides,the role and importance of self-assembled peptide hydrogels,and a brief overview on molecular simulation of self-assembled peptide hydrogels applied for bone and dental tissue engineering and regeneration.

摘要：Scavenging reactive oxygen species(ROS) by antioxidants is the important therapy to cerebral ischemia-reperfusion injury(CIRI) in stroke. The antioxidant with novel dual-antioxidant mechanism of directly scavenging ROS and indirectly through antioxidant pathway activation may be a promising CIRI therapeutic strategy. In our study, a series of chalcone analogues were designed and synthesized, and multiple potential chalcone analogues with dual antioxidant mechanisms were screened. Among these compounds, the most active 33 not only conferred cytoprotection of H2 O2-induced oxidative damage in PC12 cells through scavenging free radicals directly and activating NRF2/ARE antioxidant pathway at the same time, but also played an important role against ischemia/reperfusion-related brain injury in animals. More importantly, in comparison with mono-antioxidant mechanism compounds, 33 exhibited higher cytoprotective and neuroprotective potential in vitro and in vivo. Overall, our findings showed compound 33 couldemerge as a promising anti-ischemic stroke drug candidate and provided novel dual-antioxidant mechanism strategies and concepts for oxidative stress-related diseases treatment.

摘要：Objective To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses(HEVs)from clinical samples and to contribute to etiological surveillance of HEV-related *** A panel of RT-nPCR assays,consisting of published combined primer pairs for VP1 genes of HEV A–C and in-house designed primers for HEV-D,was established in this *** sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID50 perμL and copies perμL,and the newly established methods were tested in clinical specimens collected in recent *** The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID50 perμL and 10 virus copies perμL,and for the complete VP1 gene was 1 CCID50 perμL and 100 virus copies perμL,using serially-diluted virus stocks of five *** a proof-of-concept,25 serotypes were identified and complete VP1 sequences of 23 serotypes were obtained by this system among 858 clinical specimens positive for HEVs during the past eight surveillance *** This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A–D,providing rapid,sensitive,and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens.

摘要：Convolutional Neural Networks(CNNs)are widely used in computer vision,natural language processing,and so on,which generally require low power and high efficiency in real ***,energy efficiency has become a critical indicator of CNN *** that asynchronous circuits have the advantages of low power consumption,high speed,and no clock distribution problems,we design and implement an energy-efficient asynchronous CNN accelerator with a 65 nm Complementary Metal Oxide Semiconductor(CMOS)*** the absence of a commercial design tool flow for asynchronous circuits,we develop a novel design flow to implement Click-based asynchronous bundled data circuits efficiently to mask layout with conventional Electronic Design Automation(EDA)*** also introduce an adaptive delay matching method and perform accurate static timing analysis for the circuits to ensure correct *** accelerator for handwriting recognition network(LeNet-5 model)is *** test results show that the asynchronous accelerator has 30%less power in computing array than the synchronous one and that the energy efficiency of the asynchronous accelerator achieves 1.538 TOPS/W,which is 12%higher than that of the synchronous chip.