摘要：Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae *** Response surface methodology(RSM),combining Plackett–Burman design(PBD),steepest ascent method,and Box–Behnken design(BBD),was *** significant factors contributing to polysaccharide production were determined by *** path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum *** and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal *** The significant factors contributing to polysaccharide production were enzyme concentration,extraction time,and liquid-to-solid *** optimal conditions for maximal TFP were:enzyme concentration of2.8%,extraction time of41min,and liquid-to-solid ratio of27mL/*** experimental yield of3.08%was in good agreement with the model-predicted yield of3.1%under the optimized *** This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide,which can contribute to its further production and application.