摘要：The water vapor permeability(WVP)of films is important when developing pharmaceutical *** are frequently used as coatings,and as such directly influence the quality of the *** optimization of processing conditions for sodium alginate films was investigated using response surface ***-factor tests and Box-Behnken experimental design were *** was selected as the response variable,and the operating parameters for the single-factor tests were sodium alginate concentration,carboxymethyl cellulose(CMC)concentration and CaCl2solution immersion *** coefficient of determination(R2)was 0.97,indicating statistical significance.A minimal WVP of0.389 8 g·mm/(m2·h·kPa)was achieved under the optimum *** were found to be a sodium alginate concentration,CMC concentration and CaCl2solution immersion time at 8.04%,0.13%,and12 min,*** provides a reference for potential applications in manufacturing film-coated hard capsule shells.

摘要：基于X射线纳米闪烁体的光响应疗法是一种新兴的有良好应用前景的活体肿瘤治疗策略,迫切需要开发具有高效X射线激发紫外发光性能的纳米闪烁体.然而,由于当前纳米闪烁体的X射线紫外发光性能仍较弱,实现高效的X射线激活肿瘤治疗仍然是一个巨大的挑战.为此,我们发展了一种新型的具有良好X射线紫外发光性能的Gd^(3+)/Ce^(3+)共掺杂的LiLuF4纳米闪烁体,并将其应用于活体肿瘤治疗.通过优化基质材料、掺杂剂和能量传递设计合成的纳米闪烁体,其X射线紫外发光强度比传统的Ce^(3+)单掺杂材料增强了约18倍.我们进一步将纳米闪烁体与一氧化氮(NO)前体结合以概念验证研究纳米闪烁体的应用.在X射线照射下,该纳米复合物能够可控地产生NO,并在X射线诱导的NO和放射治疗协同作用下实现了优异的抗肿瘤效果.此外,X射线激活的NO治疗可以抑制肿瘤向肝脏转移,抑制肿瘤再生,延长小鼠存活率.这项工作有望推动纳米闪烁体的发展及其在活体深层组织疾病治疗中的应用.

摘要：In the application of RNAi technology,it is an essential step to design siR NA applicable to target *** present,there are many researches and conclusions on siR NA *** paper aims to the influences of mR NA secondary structure or siR NA antisense-strand secondary structure on siR NA silence *** paper also discusses the problems and sets out further insights in the research.

摘要：通过多重miRNA触发的原位siRNA递送治疗策略可以有效提高对癌细胞的精确治疗.基于DNA纳米结构的可编程性、特异性分子识别、易于功能化修饰和良好生物相容性的特点,我们设计了一个3D DNA纳米治疗平台,用于实现双miRNA触发响应的siRNA原位递送.这种3D DNA纳米结构(TY1Y2)是由DNA四面体支架、两组Y型DNA(Y1和Y2)和EpCAM核酸适体通过自组装构建而成的.TY1Y2被特异性内化至靶标癌细胞后,能够被两个内源性miRNA(miR-21和miR-122)触发,从而产生强的荧光共振能量转移信号,用于双miRNAs成像.同时,治疗性的siRNA(siSurvivin和siBcl2)也可以通过链置换反应从TY1Y2中产生并进行原位释放,实现癌细胞的协同基因治疗.这种3D DNA纳米结构将内源性生物标记物的特异性成像和治疗性基因的原位递送整合为一个多功能纳米平台,在癌症诊断和治疗方面显示出广阔的应用前景.

摘要：The band conveyer driven by linear friction is a new device. It can reduce drivesize and conveyor belt tensity, and increase delivery capacity. It has feasibility and usability particularly in altering the original conveyer and solving the problems of capacity insufficiency. The technology has brought certain difficulty for engineers, because it has certaindifficulty both in theory and in calculation. Therefore, Visual Basic 6.0 programming technology was used to develop a set of 'the design system of the band conveyer driven bylinear friction.' After being proved in the field, it can completely meet the demands of thedesign. This paper introduced its main theory or basis in design, so as to provide relatedtechnical support to this kind of project.

摘要：A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of *** three independent variables were withering time,fermentation time and fermentation *** factor was set by three *** responses were predicted by analysis of the regression *** the study,the models of regression equation were significant by the analysis of variance(ANOVA).The second-order response surface figures were showed that interaction term is correlated with the *** process parameters for the maximum of TF and TR formation were obtained at withering time of 12 hours,fermentation time of 2 hours,and fermentation temperature of 30℃.The predictions of TF and TR formation were 0.52% and 3.98%.The results obtained in this study showed that the CCD and Response Surface Methodology(RSM) could be an effective tool to optimize the process parameters of Congou black tea manufacture.

摘要：Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment. To accomplish this, we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation. This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer, AS1411,and a replaceable anti-biomarker probe(ABP). We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS 1411 and then activated by the presence of a given intracellular biomarker. By using such a belt-and-braces strategy, this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells. Moreover, since the expression level of biomarkers tends to indicate the specific physiological state of individual cells, biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level.

摘要：目的:基于对针刺治疗多囊卵巢综合征随机对照临床试验现状的系统总结和分析,提出对未来相关临床研究的建议。创新点:首次对目前针刺治疗多囊卵巢综合征随机对照临床试验进行了全面的总结和分析,从研究设计和报告规范等方面提出了一系列改进的建议。方法:对MEDLINE、EMBASE、万方和知网等八个数据库进行了针刺治疗多囊卵巢综合征相关文献的全面检索,按照纳入标准将文章进行分类和总结,并对每项研究进行了详细的质量评估。根据当前的研究情况对未来针刺治疗多囊卵巢综合征的随机对照临床试验提出了一系列建议。结论:从如何完善实验设计,提高研究质量,加强报告规范等方面提出了一系列改进的建议,对未来针刺治疗多囊卵巢综合征随机对照试验的设计和实施提供了指导和帮助。

摘要：目的:优化低温脱脂大豆粕中的低丰度蛋白提取和高丰度蛋白去除条件,为进一步探讨大豆低丰度蛋白对动物的生理功能影响提供试验材料。创新点:结合提取时间和异丙醇浓度,将超声波(超声时间、功率)用于辅助异丙醇去除大豆高丰度蛋白和富集低丰度蛋白的研究。方法:在单因素试验基础上,设计了超声时间、超声功率、提取时间和异丙醇浓度等四因素三水平的正交试验。并通过测定提取液中的蛋白浓度和通过聚丙烯酰胺凝胶电泳(SDS-PAGE)、质谱分析(MS)等蛋白质组学手段鉴定提取液或丙酮沉淀物中的蛋白质含量,实现了大豆高丰度蛋白去除和低丰度蛋白提取条件的优化。结论:50%异丙醇,350 W超声15 min,提取1 h是大豆低丰度蛋白提取的最佳条件。提取物含多种低丰度蛋白(油质蛋白、大豆未知蛋白、磷酸酯D和无特征大豆蛋白),且无大豆高丰度蛋白(大豆球蛋白和β-伴大豆球蛋白)。

摘要：目的:通过壳聚糖和那他霉素在果蔬生理代谢和致病微生物抑制等方面的特点,达到减缓毛酸浆果实在贮藏期间的生理衰老和抑制致病菌发展,进而提高毛酸浆贮藏品质。创新点:壳聚糖作为一种涂被剂,可以均匀地分布在果蔬表面。许多学者在研究中发现壳聚糖可以延缓果蔬的生理代谢。那他霉素作为一种真菌抑制剂,通常和涂被剂联合用于奶酪的贮藏防霉。本文的创新在于壳聚糖和那他霉素联合在毛酸浆贮藏中的应用。方法:按比例制备出壳聚糖水溶液,随后定量添加那他霉素并搅拌均匀。毛酸浆果实在浸泡一定时间后,捞出沥干。随后按照实验设计进行分组处理。在贮藏期内,定期测定菌落总数对数值、果实外部色差、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)酶活性、丙二醛(MAD)含量及感官评价等指标。最后进行总结分析。结论:单独使用壳聚糖时,可以延缓毛酸浆果实的生理衰老,但是难以抑制贮藏期间的致病微生物(主要是真菌类);作为一种真菌抑制剂,那他霉素具有水溶性低的特点,难以单独使用。那他霉素与壳聚糖联合使用时,壳聚糖即可对毛酸浆果实起到生理作用,还可以作为那他霉素的载体,使其均匀分布在果实表面。二者联合使用既能延缓毛酸浆果实的生理衰老,又能抑制贮藏期间的致病菌,从而达到提高毛酸浆贮藏品质的目的。