摘要：To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP),TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating *** TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo,and fluorescent microscopy,alizarin red staining,paraffin sectioning,scanning and transmission electron microscopy observations in *** corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune *** corneal thickness of transplanted eyes decreased gradually after transplanting,reaching almost the thickness of normal eyes after 156 d,while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal *** polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE *** intact monolayer corneal endothelium had been reconstructed with the morphology,cell density and structure similar to those of normal rabbit corneal *** conclusion,the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP *** TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

摘要：类 Vibrio 原因 vibriosis 的病原的种， maricultured 动物和海味消费者的最流行的疾病之一。在海味生产的链监视他们的动力学，处理并且消费为食物和海洋生物养殖安全是很重要的。为了充实代表 Vibrio 16S ribosomal RNA 基因(rDNA ) ，碎裂并且识别进一步真实及时的这些细菌并且同时地在在链的细菌的植物群之中，有选择地放大 Vibrio 16S rDNA 碎片的一双教材与他们在海水 Vibrio 的分析证明的特性和报道被设计社区。二份教材， VF169 和 VR744 的 Thespecificities 和范围，被使用强风节目在在 GenBank 可得到的细菌的 16S rDNAs 之中理论上决定，实际上，由放大 Vibrio16S， rDNA 从海水 DNA 碎裂。超过 88.3% 序列在 GenBank，与 VR744 显示出相同火柴，属于 Vibrio 类。33 克隆的一个总数随机被选择并且定序。所有序列显示出他们的最高的类似到并且多样的已知的 Vibrio 种类在那些附近聚类。设计的教材能够检索宽范围 ofVibrio 16S rDNA 在海水在细菌的植物群之中明确地碎裂，海味耕作的最重要的自然环境。