摘要：A fluorescent aminoacid was designed for selective and sensitive detection of Cu(II) in aqueous solution. The designing of this Cu(II) fluorescent chemosensing molecule, N (1 naphthyl)~aminoacetic acid (NAA), was based on the binding of Cu(II) to aminoacetic acid and the novel charge transfer photophysics of 1 aminonaphthalenes. The fluorescence of NAA was found quenched by Cu(II) and several other metal ions of similar electronic structure such as Co(II), Ni(II) and Zn(II). The quenching was shown to occur via electron transfer within the metal NAA complex, which required an optimal combination of high binding affinity and favorable redox properties of the components in the metal NAA complex and hence afforded selective fluorometric detection of Cu(II). The calibration graph obeyed Stern Volmer theory and was shown for Cu(II) over the range of 0—2 75×10 -4 mol/L. The quenching constant of Cu(II) was measured as 8 0×10 3 mol/L that was two orders of magnitude higher than those of Co(II), Ni(II) and Zn(II). The 3SD limit of detection for Cu(II) was 8 00×10 -6 mol/L with a coefficient of variation of 1 65%. Linear range for quantitative detection of Cu(II) was 2 67×10 -5 —2 75×10 -4 mol/L. The method was applied to synthetic sample measurements which gave recoveries of 105%—112%.

摘要：目前,硫化矿是贵金属的主要来源。在硫化矿处理过程中存在的主要问题之一是其在酸中的溶解度低(K_(sp)<10^(-20)),并产生有毒有害的副产品。在本研究中,使用高锰酸铝[Al(MnO_(4))_(3)]氧化剂溶解硫化矿来提取金属,部分解决了这个问题。基于Aspen Hysys模型组装的Taguchi实验设计作为一种从实验到工业规模的新方案,已应用于Al(MnO_(4))_(3)氧化剂对硫化物矿石的溶解研究。最佳结果已被用作Aspen Hysys软件对该过程进行模拟和灵敏度分析的主要数据。研究了pH、停留温度、搅拌速率、停留时间、Al(MnO_(4))_(3)消耗量、浸出密度、粒度和氧压等参数对从硫化矿中萃取金属效率的影响。在优化条件下,锌、铜和铅的萃取率分别达到77%、73%和70%。

摘要：A factorial experimental design was combined with response surface methodology(RSM) to opti-mize the catalyzed CO2 consumption by coke deposition and syngas production during the dry re-forming of CH4. The CH4 /CO2 feed ratio and the reaction temperature were chosen as the variables, and the selected responses were CH4 and CO2 conversion, the H2 /CO ratio, and coke deposition. The optimal reaction conditions were found to be a CH4 /CO2 feed ratio of approximately 3 at 700 °C, producing a large quantity of coke and realizing high CO2 conversion. Furthermore, Raman results showed that the CH4 /CO2 ratio and reaction temperature affect the system's response, particularly the characteristics of the coke produced, which indicates the formation of carbon nanotubes and amorphous carbon.

摘要：研究目的:通过综述作物对纳米材料的吸收途径和积累,以及纳米材料对农作物生长和营养的影响,为纳米污染在农业中的风险提供理论分析和启示。创新要点:归纳了纳米材料被作物吸收的路径和对作物生理、遗传、营养各水平上产生的胁迫。重要结论:当前纳米与作物的研究应集中在食品安全相关的问题上,考虑农业实际情况和环境因素,分析纳米材料通过食物链富集和传递的可能性,探讨纳米材料与其他土壤有机污染物可能产生的复合污染。

摘要：Nanozymes, nanomaterials with enzyme-like characteristics,are emerging as novel artificial enzymes (Gao et al., 2007;Manea et al., 2004;Yan, 2018). They are superior to natural enzymes in many ways, such as higher stability, lower cost in preparation, and better robustness toward harsh environments (Wei and Wang, 2013).

摘要：At present,the clinical reconstruction of the auricle usually adopts the strategy of taking autologous costal *** method has great trauma to patients,poor plasticity and inaccurate ***-dimensional(3D)printing technology has made a great breakthrough in the clinical application of orthopedic *** study explored the combination of 3D printing and tissue engineering to precisely reconstruct the ***,a polylactic acid(PLA)polymer scaffold with a precisely customized patient appearance was fabricated,and then auricle cartilage fragments were loaded into the 3D-printed porous PLA scaffold to promote auricle *** vitro,gelatin methacrylamide(GelMA)hydrogels loaded with different sizes of rabbit ear cartilage fragments were studied to assess the regenerative activity of various autologous cartilage *** vivo,rat ear cartilage fragments were placed in an accurately designed porous PLA polymer ear scaffold to promote auricle *** results indicated that the chondrocytes in the cartilage fragments could maintain the morphological phenotype in *** three months of implantation observation,it was conducive to promoting the subsequent regeneration of cartilage in *** autologous cartilage fragments combined with 3D printing technology show promising potential in auricle reconstruction.

摘要：Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment. To accomplish this, we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation. This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer, AS1411,and a replaceable anti-biomarker probe(ABP). We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS 1411 and then activated by the presence of a given intracellular biomarker. By using such a belt-and-braces strategy, this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells. Moreover, since the expression level of biomarkers tends to indicate the specific physiological state of individual cells, biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level.

摘要：Nanozymes,the functional nanomaterials with enzyme-like activities,have been widely explored for both in vitro and in vivo applications(Wei and Wang,2013;Wu et al.,2019;Huang et al.,2019;Liang and Yan,2019).Particularly,by modulating the levels of reactive oxygen species(ROS)in vivo,numerous therapeutic nanozymes have been developed to treat diseases,ranging from cancer to neurodegenerative diseases(Fan et al.,2018;Kwon et al.,2018).

摘要：In order to enhance the glutathione peroxidase（GPX） catalytic activity of the selenium-containing single-chain variable fragments（Se-scFv）, a novel human scFv was designed on the basis of the structure of human antibody and optimized via bioinformatics methods such as homologous sequence analysis, three-dimensional（3D） model building, binding-site analysis and docking. The DNA sequence of the new human scFv was synthesized and cloned into the expression vector pET22b（＋）, then the scFv protein was expressed in soluble form in Escherichia coli BL21（DE3） and purified by Ni2＋-immobilized metal affinity chromatography（IMAC）. The serine residue of scFv in the active site was converted into selenocysteine（Sec） with the chemical modification method, thus, the human Se-scFv with GPX activity was obtained. The GPX activity of the Se-scFv protein was characterized. Compared with other Se-scFv, the new human Se-scFv showed similar efficiency for catalyzing the reduction of hydrogen peroxide by glutathione. It exhibited pH and temperature dependent catalytic activity and a typical ping-pong kinetic mechanism.

摘要：Bladder cancer is the most common malignant tumor in the urinary system,with high morbidity,mortality and recurrence after ***,current bladder cancer urine diagnosis methods are limited by the low accuracy and specificity due to the low abundance of bladder cancer biomarkers in the urine with complex biological ***,we present a high stability indium gallium zinc oxide field effect transistor(IGZO-FET)biosensor for efficient identification of bladder cancer biomarkers from human urine *** recognition molecular functionalized IGZO-FET biosensor exhibits stable electronic and sensing performance due to the large-area fabrication of IGZO thin-film *** to the excellent electrical performance of IGZO-FET,the IGZO-FET biosensor exhibits high sensitivity and extremely low detection limit(2.7 amol/L)towards bladder cancer *** IGZO-FET biosensor is also able to directly detect bladder tumor biomarker in human urine with high sensitivity and specificity,and could differentiate bladder cancer patients’urine samples from healthy donors *** results indicate that our designed high-performance biosensor shows great potential in the application of portable digital bladder cancer diagnosis devices.