摘要：Skin acts as protective barrier against a number of factors such as dust,opportunistic microbial and viral infections,regulates body temperature and waste *** cell population plays an important role in devclopment of skin architecturc.A scaffold having capability to support and enhance fibroblast growth is a viable option for wound dressing material which can shorten the time for wound to *** this work,Silk Fibroin(SF)and Xanthan(Xa)were blended in three ratios 80 SF:20 Xa(SFX82),60 SF:40 Xa(SFX64),and 50SF:50 Xa(SFX55)to create SF/Xa *** and other physicochemical properties of SF/Xa scaffold are functions of blending ratios and blend with the ratio 80 SF:20 Xa has the highest *** properties of SF/Xa blends are a function of miscibility with SFX82 having superior thermal propertis of all fabricated *** porosity of SF/Xa scaffolds is in the range of 67%to 50%,with pore size of 58.1 um-45.5 um,water uptake capacity of 92%-86%,and surface roughness of 49.95 nm-385 ***82 shows highest growth rate of L929 fibroblast cells indicating its superiority over other scaffolds for providing biological cues for the growth and proliferation of fibroblastic cells in natural ***82 scaffold is found to be most suitable for fibroblastic cells thereby enhancing the tissue regeneration at wound site.

摘要：This review offers an overview of the latest developments in metal-covalent organic framework(MCOF)and covalent metal-organic framework(CMOF)materials,whose construction entails a combination of reversible coordination and covalent bonding adapted from metal-organic frameworks(MOFs)and covalent organic frameworks(COFs),*** an emphasis on the MCOF and CMOF structures,this review surveys their building blocks and ***,the frameworks are classified based on the dimensions of their components(building blocks),namely,discrete building blocks and one-dimensional infinite building *** the first category,the materials are further divided into collections of two-and three-dimensional networks based on their *** the second category,the recently emerging MCOFs with woven structures are ***,the state-of-the-art in MCOF and CMOF chemistry has been laid out for promising avenues in future developments.

摘要：Microalgal oils, depending on their degree of unsaturation, can be utilized as either nutritional supplements or fuels; thus, a feedstock with genetically designed and tunable degree of unsaturation is desirable to maximize process efficiency and product versatility. Systematic profiling of ex vivo （in yeast）, in vitro, and in vivo activities of type-2 diacylglycerol acyltransferases in Nannochloropsis oceanica （NoDGAT2s or NoDGTTs）, via reverse genetics, revealed that NoDGAT2A prefers saturated fatty acids （SFAs）, NoDGAT2D prefers monounsaturated fatty acids （MUFAs）, and NoDGAT2C exhibits the strongest activity toward polyunsaturated fatty acids （PUFAs）. As NoDGAT2A, 2C, and 2D originated from the green alga, red alga, and eukaryotic host ancestral participants of secondary endosymbiosis, respectively, a mecha- nistic model of oleaginousness was unveiled, in which the indigenous and adopted NoDGAT2s formulated functional complementarity and specific transcript abundance ratio that underlie a rigid SFA：MUFA：PUFA hierarchy in triacylglycerol （TAG）. By rationally modulating the ratio of NoDGAT2A＇：2C^D transcripts, a bank of N. oceanica strains optimized for nutritional supplement or fuel production with a wide range of degree of unsaturation were created, in which proportion of SFAs, MUFAs, and PUFAs in TAG varied by 1.3-, 3.7-, and 11.2-fold, respectively. This established a novel strategy to simultaneously improve productivity and quality of oils from industrial microalgae.

摘要：Existing therapies for the treatment of chronic heart failure still have some limitations and there is a pressing need for the development of new therapeutic modalities. The amniotic membrane has been used for the treatment of various diseases, such as conjunctive defects;however, the mechanisms behind its repair functions are still unclear. Regenerative medicine is seeking newer alternatives and among them, biomaterials have emerged in recent years for developing and manipulating molecules, cells, tissues or organs grown in laboratories in order to replace human body parts. Many such materials have been used for this purpose, either synthetically or biologically, in order to provide new medical devices. This review provides a wider view of the regeneration potential of the use of amniotic membrane as a potential biomaterial to facilitate the implementation of new research in surgical procedures. Amniotic membrane appears to be an alternative source of stem cells as well as an excellent biomaterial for cell-based therapeutic applications in engineering heart tissue.

摘要：The majority of marine microbes remain uncultured,which hinders the identification and mining of CO_(2)-fixing genes,pathways,and chassis from the ***,we investigated CO_(2)-fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their ^(13)C-bicarbonate(^(13)C-HCO_(3)^(-))intake via single-cell Raman spectra(SCRS)*** target cells were then isolated by Raman-activated Gravity-driven Encapsulation(RAGE),and their genomes were amplified and sequenced at one-cell *** single-cell metabolism,phenotype and genome are *** identified a notyet-cultured Pelagibacter spp.,which actively assimilates ^(13)C-HCO_(3)^(-),and also possesses most of the genes encoding enzymes of the Calvin-Benson cycle for CO_(2) fixation,a complete gene set for a rhodopsin-based light-harvesting system,and the full genes necessary for carotenoid *** four proteorhodopsin(PR)genes identified in the Pelagibacter *** confirmed by heterologous expression in *** results suggest that hitherto uncultured Pelagibacter *** light-powered metabolism to contribute to global carbon cycling.