摘要：As surgical procedures transition from conventional resection to advanced tissue-regeneration technologies,human disease therapy has witnessed a great leap *** particular,three-dimensional(3D)bioprinting stands as a landmark in this setting,by promising the precise integration of biomaterials,cells,and bioactive molecules,thus opening up a novel avenue for tissue/organ *** by the editorial board of Bio-Design and Manufacturing,this review brings together a cohort of leading young scientists in China to dissect the core functionalities and evolutionary trajectory of 3D bioprinting,by elucidating the intricate challenges encountered in the manufacturing of transplantable *** further delve into the translational pathway from scientific research to clinical application,emphasizing the imperativeness of establishing a regulatory framework and rigorously enforcing quality-control ***,this review outlines the strategic landscape and innovative achievements of China in this field and provides a comprehensive roadmap for researchers worldwide to propel this field collectively to even greater heights.

摘要：Vortex diode,as an important component in power fluidics,has been used in nuclear reprocessing engineering where it is desirable to avoid pumps with moving *** performance of the fluidic system depends on the matched design parameters of vortex diodes and the supporting ***,no clear guidelines for design and optimization of vortex diodes system are ***,we carried out detailed experimental study on hydraulic characteristics of the vortex diodes system with changeable parameters to evolve such *** study covered a wide range of a vortex diode and vortex diodes *** variation of averaged discharge and volumetric efficiency in respect to air supply pressure,liquid level of water tank and liquid level stroke of gas-liquid energy converter was *** experimental results were analyzed to develop design *** guidelines are shown to be useful for obtaining the design parameters that would yield the desired hydrau- lic performance for the required operating conditions.

摘要：Factorial design and response surface techniques were used to design and optimize increasing P450 BM-3 expression in E, coll. Operational conditions for maximum production were determined with twelve parameters under consideration： the concentration of FeCl3, induction at OD578 （optical density measured at 578 nm）, induction time and inoculum concentration. Initially, Plackett-Burman （PB） design was used to evaluate the process variables relevant in relation to P450 BM-3 production. Four statistically significant parameters for response were selected and utilized in order to optimize the process. With the 416C model of hybrid design, response surfaces were generated, and P450 BM-3 production was improved to 57.90×10^-3 U/ml by the best combinations of the physicochemical parameters at optimum levels of 0,12 mg/L FeCl3, inoculum concentration of 2.10%, induction at OD578 equal to 1.07, and with 6.05 h of induction.

摘要：Natural muscle provides excellent motilities for *** the basic unit of the muscle system,the skeletal muscle fibers function as a soft linear *** by the muscle fibers,researchers have developed various soft active devices with linear *** paper reviews several soft linear actu ators,such as the dielec trie elastomer,t hermal responsive hydrogels,pneumatic artificial muscle,and conducting *** actuation mechanisms and performances of these soft linear actuators are *** on the dielectric elastomer,we propose a design of a hybrid system with linear actuation,driven by both the electric motor and dielectric elasto mer *** elec tromechanical behaviors of the dielec trie elastomer cone have been investigated in both experiment and finite element *** work may guide the further design of soft actuators and robots.

摘要：High-energy rotor uncontained failure can cause catastrophic damage effects to aircraft systems if not addressed in design. In this paper, numerical simulations of three high-energy rotor disk fragments impacting on U type protection rings are carried out using LS-DYNA. Protection rings with the same mass and different groove depths are designed to study the influence of the groove depth. Simulation results including kinetic energy and impact force variation of single frag- ment are presented. It shows that the groove depth infects both the axial containment ability of the protection ring and the transfer process of energy. The depth of groove ought to be controlled to an appropriate value to meet both the requirement of axial containment and higher safety factor. Ver- ification test on high-speed spin tester has been conducted and shows that protection ring with appropriate U structure can resist the impact of the disk burst fragments. The ring is inflated from a circular to an oval-triangle shape. The corresponding simulation shows good agreement with the test.

摘要：Three-layer feedforward networks have been widely used in modeling chemical engineering processes and prior-knowledge-based methods have been introduced to improve their *** this paper,we propose the methodology of designing better prior-knowledge-based hybrid methods by combining the existing ones. Then according to this methodology,two hybrid methods,interpolation-optimization (IO) method and interpolation penalty-function (IPF) method,are designed as ***,both methods are applied to modeling two cases in chemical engineering to investigate their *** results show that the performances of the hybrid methods are better than those of their parents.

摘要：This article deals with the design of energy efficient water utilization systems allowing operation split. Practical features such as operating flexibility and capital cost have made the number of sub operations an important parameter of the problem. By treating the direct and indirect heat transfers separately, target freshwater and energy consumption as well as the operation split conditions are first obtained. Subsequently, a mixed integer non-linear programming （MINLP） model is established for the design of water network and the heat exchanger network （HEN）. The proposed systematic approach is limited to a single contaminant. Example from literature is used to illustrate the applicability of the approach.

摘要：Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.

摘要：Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two *** development of adsorbents for EBA process is a challenging course,with the special design and preparation according to the target molecules and specific expanded bed *** types of supporting matrices for expanded bed adsorbents have been developed,and their preparation methods are being consummated *** matrices are activated and then coupled with ligands to form functionalized adsorbents,including ion-exchange adsorbents,affinity adsorbents,mixed mode adsorbents,hydrophobic charge induction chromatography adsorbents and *** this review,the preparation of the matrices for EBA process is summa-rized,and the coupling of ligands to the matrices to prepare functionalized adsorbents is discussed as well.

摘要：Dextran-hyaluronate （Dex-HA） based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate （Dex-MA） and hyaluronate methacrylate （HA-MA） at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concen- tration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% （w/w） at the HA-MA concentration of 1% （w/v）, which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% （w/w） macromonomer solution had high water permeability （5.1 × 10 ^-2m2） and high porosity （92.4%）. The pore diameter examined by scanning electron microscopy （SEM） was in a broad range of 50-135 um with the mean pore diameter of 91 um. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.