摘要：Autologous bone marrow-derived mesenchymal stem cells(BMSCs)have been shown to promote osteogenesis;however,the effects of allogeneic BMSCs(allo-BMSCs)on bone regeneration remain ***,we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle(ABP)***,we concurrently printed scaffolds with polycaprolactone,ABPs,and allo-BMSCs for appropriate support,providing bioactive factors and seed cells to promote *** vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic *** vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted *** experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells,chondrocytes,and *** implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect,promoting bone *** study contributes to our understanding of allo-BMSCs,providing information relevant to their future application.

摘要：A series ofN-（furan-2-ylmethyl）-lH-indole-3-carboxamide derivatives（6a--6p） was designed and synthe- sized for developing novel indole scaffolds as anticancer agents targeting the epidemal growth factor receptor （EGFR）, and the cytotoxic activities of the target compounds were evaluated against three EGFR high-expressed cancer cell lines[human lung adenocarcinoma cell line（A549）, Henrietta Lacks strain of cancer cell line（HeLa） and human colorectal cancer cell line（SW480）], one EGFR low-expressed cell line（human liver cancer cell line, HepG2） and one human liver normal cell line（HL7702） using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay, Some target compounds exhibited potent anficancer activities against A549, HeLa, SW480 and weak activities on HepG2, which signifies that the target compounds are likely to be EGFR inhibitors as expected. And they showed weak cytotoxic effects on HL7702, which implies the target compounds are probably to be of low toxicity against normal cells. Among them, the target compound 1-ethyl-N-（fttran-2-ylmethyl）-5-{2-{ [2-（2-methoxy- phenoxy）ethyl]amino}-2-oxoethoxy}-2-methyl-1H-indole-3-carboxamide（6p） with 2-{[2-（2-methoxyphenoxy）ethyl]- amino}-2-oxoethoxy group at the C5 position of the N-（furan-2-ylmethyl）-lH-indole-3-carboxamide scaffold exhibited the most potent anticancer activity. Also the binding interaction of the target compound 6p with EGFR was explored by molecular docking. Conclusively, the novel indole scaffold may be beneficial to investigate new anticancer agents for targeting the EGFR.

摘要：This paper mainly discusses the power and solidarity among participants in the community correction discourse from sociolinguistic perspective by adopting the methodology of conversation *** the comparative study of the steps of Initial Evaluation and Entry Ceremony in community correction,this paper analyses the power and solidarity in the features of adjacency pair and turn-taking,and finds that the step of Initial Evaluation is relatively negotiable and represents the social relationship of solidarity,while the step of Entry Ceremony stresses more on social distance and power *** reasons that cause the power and solidarity in the Initial Evaluation and the Entry Ceremony from the perspective of social identity and social purposes are also explored.

摘要：The aim of this paper is to use a new vision in interpreting Du Fu＇s disease poem in order to build a more profound interpretation in the tradition of Chinese Lyricism. As the illness experiences are irreplaceable and the disease poems expressing clear self-image of the poet, we attempt to explore the unique illness experiences to uncover the lyrical self＇ in Du Fu＇s poems. The perception of the world of the poets suffering from illness would be different from the past while they were healthy, and thus they were experiencing a new sense of ＂space＂ through replacing the ordinary sense of space. This paper is divided into three parts： the first part is to interpret the new sense of space that is exhibiting features of anxiety caused by the state of imprisoned and the lost of mobility in life that is experienced by Du Fu＇s while he was sick. The second part of this study attempts to switch the perspective of Du Fu＇s disease poems that constructed a new sense of space by the action of＂ascending＂ in order to inspect whether the patient was able to overcome his anxiety while standing on a higher plane. The third part of this paper is to study the posture of＂lying＂, examining Du Fu expression of space while suffering from illness and had to stay at a lower position, and his perception of the space while suffering from chronic illness.

摘要：Traditional tumor models do not tend to accurately simulate tumor growth in vitro or enable personalized treatment and are particularly unable to discover more beneficial targeted *** address this,this study describes the use of threedimensional(3D)bioprinting technology to construct a 3D model with human hepatocarcinoma SMMC-7721 cells(3DP-7721)by combining gelatin methacrylate(GelMA)and poly(ethylene oxide)(PEO)as two immiscible aqueous phases to form a bioink and innovatively applying fluorescent carbon quantum dots for long-term tracking of *** GelMA(10%,mass fraction)and PEO(1.6%,mass fraction)hydrogel with 3:1 volume ratio offered distinct pore-forming characteristics,satisfactorymechanical properties,and biocompatibility for the creation of the 3DP-7721 *** analysis and quantitative real-time fluorescence polymerase chain reaction(PCR)were used to evaluate the biological properties of the *** with the two-dimensional culture cell model(2D-7721)and the 3D mixed culture cell model(3DM-7721),3DP-7721 significantly improved the proliferation of cells and expression of tumor-related proteins and ***,we evaluated the differences between the three culture models and the effectiveness of antitumor drugs in the three models and discovered that the efficacy of antitumor drugs varied because of significant differences in resistance proteins and genes between the three *** addition,the comparison of tumor formation in the three models found that the cells cultured by the 3DP-7721 model had strong tumorigenicity in nude *** evaluation of the levels of biochemical indicators related to the formation of solid tumors showed that the 3DP-7721 model group exhibited pathological characteristics of malignant tumors,the generated solid tumors were similar to actual tumors,and the deterioration was *** research therefore acts as a foundation for the application of 3DP-7721 models in drug devel

摘要：Power-line interference is one of the most common noises in magnetotelluric(MT)*** usually causes distortion at the fundamental frequency and its odd harmonics,and may also affect other frequency *** trap circuits are designed to suppress such noise in most of the modern acquisition devices,strong interferences are still found in MT data,and the power-line interference will fluctuate with the changing of load *** fixed trap circuits often fail to deal with *** paper proposes an alternative scheme for power-line interference removal based on frequency-domain sparse ***,the fast Fourier transform of the acquired MT signal is ***,a redundant dictionary is designed to match with the power-line interference which is insensitive to the useful ***-line interference is separated by using the dictionary and a signal reconstruction algorithm of compressive sensing called improved orthogonal matching pursuit(IOMP).Finally,the frequency domain data are switched back to the time domain by the inverse fast Fourier *** experiments and real data examples from Lu-Zong ore district illustrate that this scheme can effectively suppress the power-line interference and significantly improve data *** with time domain sparse decomposition,this scheme takes less time consumption and acquires better results.

摘要：临床上常用的广谱抗生素无法有效解决抗生素高度耐药ESKAPE菌感染的难题,ESKAPE耐药菌已经成为人类生命健康的重大威胁.抗生素与佐剂的联合治疗是解决抗生素耐药菌感染的重要途径,但是探索对所有ESKAPE耐药菌都具有广谱和高效抗菌活性的抗生素-佐剂组合仍然面临巨大挑战.在这里,我们提出了一种抗生素-佐剂的有效设计策略,将对ESKAPE中耐药阴性菌具有高活性的多粘菌素B(PMB)与模拟宿主防御肽的细菌膜靶向阳离子聚(2-噁唑啉)相结合.我们通过2-噁唑啉单体可控聚合,合成了一系列具有不同疏水性侧链的阳离子聚(2-噁唑啉).研究表明,聚(2-噁唑啉)和PMB的组合效果随着聚(2-噁唑啉)侧链疏水性的提高呈现出从拮抗效应到协同效应的逐渐变化.其中,优选的聚(2-噁唑啉)EACA-POX_(20)显著增强了PMB的抗菌活性,尤其是对于ESKAPE耐药阳性菌的抗菌活性增强了16–32倍.聚(2-噁唑啉)EACA-POX_(20)有效增强了PMB的抗菌活性并扩展了PMB的抗菌谱.聚(2-噁唑啉)和PMB的组合策略能够有效对抗所有耐药ESKAPE病原体,为解决ESKAPE耐药菌提供了新的思路.

摘要：受电弓区域是列车气动阻力的重要来源,其贡献率约为10%,是列车气动阻力的关键区域。因此,改善受电弓区压力分布成为减小列车气动阻力的潜在有效方法。基于鞘翅目昆虫的生物形态,提出了一种新型的仿生鞘翅目导流罩,将其安装在8节编组的高速列车受电弓区域,以实现该区域的空气流动平顺。建立了原始模型(模型Ⅰ)、带导流罩的受电弓Ⅰ(模型Ⅱ)、带导流罩的受电弓Ⅱ(模型Ⅲ)、带导流罩的受电弓Ⅰ和受电弓Ⅱ(模型Ⅳ)4个计算模型,探讨列车气动减阻机理,提高列车气动性能。结果表明,安装受电弓导流罩后,受在电弓区域的气动阻力显著减小。受电弓Ⅰ区域最大减阻达84.5%,受电弓Ⅱ区域最大减阻为25.0%。当受电弓Ⅰ区域和受电弓Ⅱ区域同时安装导流罩时,受电弓Ⅰ区和受电弓Ⅱ区总减阻可达49.6%。与没有导流罩的原始模型相比,受电弓区域的空气流动更顺畅,堵塞效应更小。但是,下游流速加快,冲击风挡区域,导致气动阻力增大。在受电弓Ⅰ区域或受电弓Ⅱ区域安装导流罩时,8节列车的总阻力分别降低了4.6%和1.8%,而在受电弓Ⅰ区域和受电弓Ⅱ区域同时安装导流罩时,列车的总阻力降低了6.3%。本文可为新一代高速列车的气动设计提供参考。