摘要：DNA modified nanoparticles(Au NPs) are an established and widely used type of nucleotide sensor. We sought to improve the design by applying short rigid DNA duplexes near the surface of the Au NPs forming a so called double-anchored Au NP sensor, and compared it with other conventional DNA modified Au NPs. The improved design exhibited higher assembly efficiency, and consequently increased its sensitivity to target DNA.

摘要：The development of natural biomaterials applied for hard tissue repair and regeneration is of great importance,especially in societies with a large elderly ***-assembled peptide hydrogels are a new generation of biomaterials that provide excellent biocompatibility,tunable mechanical stability,injectability,trigger capability,lack of immunogenic reactions,and the ability to load cells and active pharmaceutical agents for tissue ***-based hydrogels are ideal templates for the deposition of hydroxyapatite crystals,which can mimic the extracellular ***,peptide-based hydrogels enhance hard tissue repair and regeneration compared to conventional *** review presents three major self-assembled peptide hydrogels with potential application for bone and dental tissue regeneration,including ionic self-complementary peptides,amphiphilic(surfactant-like)peptides,and triple-helix(collagen-like)*** attention is given to the main bioactive peptides,the role and importance of self-assembled peptide hydrogels,and a brief overview on molecular simulation of self-assembled peptide hydrogels applied for bone and dental tissue engineering and regeneration.

摘要：New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements including selectivity, stability, linearity, precision and accuracy. Chromatography was carried out using a LiChrospher RP-18 column, a mixture containing acetonitrile, phosphate buffer of pH 3.5 and methanol (45:45:10, v/v/v) and new fluorescence detection at 255 nm for excitation and 448 nm for emission. The effect of methanol content, pH of the buffer, flow rate, detection wavelengths and column temperature was estimated in robustness study, according to a plan defined by the Plackett-Burman design. For identification of significant effects, both graphical and statistical methods were used. Ro-bustness for dissolution test was checked estimating the effects of paddle speed, temperature and pH of dissolution medium. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of amlodipine and valsartan in their binary mixtures for different analytical and pharmaceutical purposes.

摘要：In order to improve the positional adaptability of our previously reported naphthyl diaryltriazines(NP-DATAs),synthesis of a series of novel biphenyl-substituted diaryltriazines(BP-DATAs)with a flexible side chain attached at the C-6 position is *** compounds exhibited excellent potency against wild-type(WT)HIV-1 with EC50 values ranging from 2.6 to 39 nmol/L and most of them showed low nanomolar anti-viral potency against a panel of HIV-1 mutant *** 5 j and 6 k had the best activity against WT,single and double HIV-1 mutants and reverse transcriptase(RT)enzyme comparable to two reference drugs(EFV and ETR)and our lead compound NP-DATA(1).Molecular modeling disclosed that the side chain at the C-6 position of DATAs occupied the entrance channel of the HIV-1 reverse transcriptase non-nucleoside binding pocket(NNIBP)attributing to the improved *** preliminary structure-activity relationship and PK profiles were also discussed.

摘要：FTY720 and IMMH002,prodrugs for sphingosine-1-phosphate receptor 1(S1P1)agonists,show inadequate and inconsistent levels of phosphorylation in humans compared to that in *** this study,FTY720 or IMMH002 analogues(21-24)were designed and synthesized with modified head pieces to improve the biotransformation of the prodrugs to the active phosphorylated *** compounds were synthesized via a convergent route using the key and optically pure building block 9,which was first synthesized via asymmetrically catalyzed *** phosphorylation rates of these analogues in rat or human blood were *** new methyl-substituted analogue compound 21 showed higher phosphorylation rates in both rats and humans than the parent compound,whereas compound 23 showed improvements in rats,but not in *** pharmacokinetics studies of rats,compounds 21 and 23 both had higher levels of phosphorylation than FTY720 and ***,our study not only yielded new compounds with therapeutic potential,but also showed species differences between rats and humans in response to the structural modifications,which might be useful for predicting the biotransformation behavior and efficacy of this class of prodrugs in the clinic.

摘要：Bladder cancer is the most common malignant tumor in the urinary system,with high morbidity,mortality and recurrence after ***,current bladder cancer urine diagnosis methods are limited by the low accuracy and specificity due to the low abundance of bladder cancer biomarkers in the urine with complex biological ***,we present a high stability indium gallium zinc oxide field effect transistor(IGZO-FET)biosensor for efficient identification of bladder cancer biomarkers from human urine *** recognition molecular functionalized IGZO-FET biosensor exhibits stable electronic and sensing performance due to the large-area fabrication of IGZO thin-film *** to the excellent electrical performance of IGZO-FET,the IGZO-FET biosensor exhibits high sensitivity and extremely low detection limit(2.7 amol/L)towards bladder cancer *** IGZO-FET biosensor is also able to directly detect bladder tumor biomarker in human urine with high sensitivity and specificity,and could differentiate bladder cancer patients’urine samples from healthy donors *** results indicate that our designed high-performance biosensor shows great potential in the application of portable digital bladder cancer diagnosis devices.

摘要：Fuel cells have attracted extensive attention due to their high conversion efficiency and environmental ***,their wider application is limited by the poor activity and high cost of platinum(Pt),which is widely used as the cathode catalyst to overcome the slow kinetics associated with oxygen reduction reaction(ORR).Pt‐based composites with one‐dimensional(1D)nanoarchitectures demonstrate great advantages towards efficient ORR *** review focuses on the recent advancements in the design and synthesis of 1D Pt‐based ORR *** introducing the fundamental ORR mechanism and the advanced 1D architectures,their synthesis strategies(template‐based and template‐free methods)are ***,their morphology and structure optimization are highlighted,followed by the superstructure assembly using 1D Pt‐based ***,the challenges and perspectives on the synthesis innovation,structure design,physical characterization,and theoretical investigations are proposed for 1D Pt‐based ORR *** anticipate this study will inspire more research endeavors on efficient ORR nanocatalysts in fuel cell application.

摘要：Synthetic polymer hydrogel nanoparticles(NPs)were developed to function as abiotic affinity reagents for *** NPs were made using both temperature-sensitive N-isopropyl acrylamide(NIPAm)and L-amino acid *** kinds of L-amino acids were acryloylated to obtain functional monomers:L-phenylalanine(Phe)and L-leucine(Leu)with hydrophobic side chains,L-glutamic acid(Glu)with negative charges,and L-lysine(Lys)and L-arginine(Arg)with positive *** incubating the NPs with fibrinogen,g-globulin,and human serum albumin(HSA)respectively,the NPs that incorporated Nacryloyl-Arg monomers(AArg@NPs)showed the strongest and most specific binding affinity to fibrinogen,when compared with g-globulin and ***,the fibrinogen-AArg binding model had the best docking scores,and this may be due to the interaction of positively charged AArg@NPs and the negatively charged fibrinogen D domain and the hydrophobic interaction between *** specific adsorption of AArg@NPs to fibrinogen was also confirmed by the immunoprecipitation assay,as the AArg@NPs selectively trapped the fibrinogen from a human plasma protein ***@NPs had a strong selectivity for,and specificity to,fibrinogen and may be developed as a potential human fibrinogen-specific affinity reagent.

摘要：Background: In the Kingdom of Saudi Arabia (KSA), recent studies revealed increasing consumption of animal products and refined foods in the diet at the expense of vegetables and fruits. These dietary changes were accused of increasing the prevalence of both overweight and obesity observed among Saudi children, adolescences and adults in the last few decades. Objectives: The present study was a cross sectional study aimed at exploring the BMI distribution among students of college of Sciences and Arts for girls Khamis Mushayt Campus 1 at King Khalid University (KKU) and investigated how Dietary habits of students female affected body mass index (BMI). Methods: A total of 240 female students aged 18 - 22 years were about 25% from total students randomly chosen from college of Arts and Science Campus 1 at Khamis Mushayt, King Khalid University, KSA for the present study. A self-reported questionnaire about the student’s dietary habits was conducted and their body mass index (BMI) was measured. Data were analyzed using SPSS statistical software and Chi-square test conducted for variables. Results: About half of the students (47%) were within normal weight, 27.4% were under weight, 16% were overweight and 9.6% were categorized obese. The majority of the students reported eating meals with their family while more than half of sample (58.7%) had eating meals during watching TV. It was worth mentioning that 84.5% of students reported eating snacks such as chocolate and chips 3 or more times per week. There were no significant differences between BMI category and dietary habits. Conclusion and Recommendations: There were no significant difference between body mass index category and dietary habits. Increasing educational programs introduced healthy dietary concepts to improve the dietary habits of female students.

摘要：Microbial cell factories (MCFs) are typical and widely used platforms in biomanufacturing for designing and constructingsynthesis pathways of target compounds in microorganisms. In MCFs, transporter engineering is especially significant forimproving the biomanufacturing efficiency and capacity through enhancing substrate absorption, promoting intracellular masstransfer of intermediate metabolites, and improving transmembrane export of target products. This review discusses thecurrent methods and strategies of mining and characterizing suitable transporters and presents the cases of transporterengineering in the production of various chemicals in MCFs.