摘要：The development of low-cost and highly efficient bifunctional electrocatalysts toward oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)is of critical importance for clean energy devices such as fuel cells and metal-air ***,a sophisticated na nostructure composed of CoS,Co and MoC nanoparticles incorporated in N and S dual-doped porous carbon nanofibers(CoS/Co/MoC-N,SPCNFs)as a high-efficiency bifunctional electrocatalyst is designed and synthesized via an efficient multistep *** as-prepared CoS/Co/MoC-N,S-PCNFs exhibit a positive half-wave potential(E1/2)of0.871 V for ORR and a low overpotential of 289 mV at 10 mA/cm^(2) for OER,outperforming the non-noble metal-based catalysts ***,the assembled Zn-air battery based on CoS/Co/MoC-N,SPCNFs delivers an excellent power density(169.1 mW/cm^(2)),a large specific capacity(819.3 mAh/g)and robust durability,demonstrating the great potential of the as-developed bifunctional electrocatalyst in practical *** work is expected to inspire the design of advanced bifunctional nonprecious metal-based electrocatalysts for energy storage.

摘要：Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from Phlebia radiata (Pr-MnP3) has been successfully engineered and amplified by polymerase chain reaction (PCR). Five mutant genes (E40H, E44H, E40H/E44H, D186H and D186N) of recombinant Phlebia radiata MnP3 (rPr-MnP3) were generated. The wild-type and the mutant genes were expressed in Escherichia coli (W3110 strain) and the resultant body proteins were lysed, purified and refolded into active enzymes. 6% - 7% recovery of pure and fully active rPr-MnP3 for wild-type and mutants were obtained and the availability of rPr-MnP3 enzymes will greatly facilitate its structure-function relationships studies. rPr-MnP3 mass was characterised using SDS-PAGE and MALDI-TOF mass spectrometry. Molecular weight of both the wild-type and mutant rPr-MnP3 enzymes was approximately 36 kDa. This describes the spectral characterization of the wild-type and mutant rPr-MnP3 enzymes with are very close similarities;substantially high spin haem enzymes. Therefore we report the engineering, cloning, expression, refolding/activation of MnP3 genes and preliminary characterization of the wild-type and mutant Phlebia radiata MnP3 enzymes.

摘要：Candida albicans （C. albicans） infection has a high mortality rate in immunocompromised patients. Owing to the inefficiency of the current diagnostic system and the absence of licensed vaccines against candidiasis, the prevention of C. albicans infection remains a challenge. C. albicans infection can be evaluated and prevented by the anti-secreted aspartyl proteinase 2 antibody （anti-Sap2 IgG） and Hsp90 antibody （anti-Hsp90 IgG）. In this study, to explore a new agent for the improvement of the diagnosis and the prevention of C. albicans infection, an engineered fd bacteriophage, which is considered a human-safe virus nanofiber, was designed and prepared with two epitopes that could induce and capture anti-Sap2 IgG and anti-Hsp90 IgG. The dual-display phage was employed as a novel capture probe to develop a new enzyme-linked immunosorbent assay （ELISA） method, which significantly improved the detection rate compared with those of the ELISA in which recombinant protein Sap2 was used as coating antigen to capture the spedfic antibodies （rSap2-ELISA） and the ELISA in which recombinant protein Hsp90 was used as coating antigen to capture the specific antibodies （rHsp90-ELISA）. In addition, the nanofibers acted as a potential vaccine to immunize mice, as well as recombinant proteins, more efficiently mediated humoral and cellular immune responses, decreased levels of C. albicans colonization, and increased the survival rates in C. albicans-infected mice. Therefore, the phage dual-display nanofiber has been shown to be a powerful bifunctional agent for protection against and sensitive detection of clinical infections, which has the potential to be widely used in the life sciences, clinical medicine, and environmental sciences.

摘要：Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug ***,the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical ***,we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile(YDLPA1)with positive charges,which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular *** comparison,zwitterionic YDLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution,but cannot polymerize in the intracellular *** polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking *** polymerization reaction induced tumor cell apoptosis,increased cell viscosity and decreased cell motility,which potentially inhibited tumor metastasis and served as a novel antitumor *** work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.

摘要：Limitations of monolayer culture conditions have motivated scientists to explore new models that can recapitulate the architecture and function of human organs more *** advances in the improvement of protocols have resulted in establishing three-dimensional(3D)organ-like architectures called‘organoids’that can display the characteristics of their corresponding real organs,including morphological features,functional activities,and personalized responses to specific *** discuss different organoid-based 3D models herein,which are classified based on their original germinal *** of organoids simulating the complexity of real tissues could provide novel platforms and opportunities for generating practical knowledge along with preclinical studies,including drug screening,toxicology,and molecular pathophysiology of *** paper also outlines the key challenges,advantages,and prospects of current organoid systems.

摘要：The purpose of this study was to fabricate decelluarized valve scaffold modified with polyethylene glycol nanoparticles loaded with transforming growth factor-β1（TGF-β1）,by which to improve the extracellular matrix microenvironment for heart valve tissue engineering in *** glycol nanoparticles were obtained by an emulsion-crosslinking method,and their morphology was observed under a scanning electron *** valve scaffolds,prepared by using trypsinase and TritonX-100,were modified with nanoparticles by carbodiimide,and then TGF-β1 was loaded into them by *** TGF-β1 delivery of the fabricated scaffold was measured by asing enzyme-linked immunosorbent *** unseeded or reseeded with myofibroblast from rats,the morphologic,biochemical and biomechanical characteristics of hybrid scaffolds were tested and compared with decelluarized scaffolds under the same *** enzyme-linked immunosorbent assay revealed a typical delivery of *** morphologic observations and biological data analysis indicated that fabricated scaffolds possessed advantageous biocompatibility and biomechanical property beyond decelluarized *** this study proved that it was feasible to fabricate the hybrid scaffold and effective to improve extracellular matrix microenvironment,which is beneficial for an application in heart valve tissue engineering.

摘要：氧化铜(CuO)具有高理论容量、丰富的资源以及生态友好等特点,被认为是一种理想的锂离子电池负极材料.然而,由于CuO本身的低导电性和循环时巨大的体积膨胀,它在电池应用中的容量利用率和循环稳定性仍然存在不足.本工作中,我们通过在甲壳素衍生的碳微球的内外壁上直接原位合成CuO纳米片,构造出一种三维多孔碳@CuO复合材料.得益于碳微球的三维多孔导电框架和CuO纳米片的合理分布,CuO的容量利用率和结构稳定性在充电/放电过程中得到明显改善,所制备的电极具有优越的循环稳定性和出色的可逆容量.本工作为合理设计三维碳/过渡金属氧化物杂化体并用作电池负极材料提供了新的思路.

摘要：Recently, the anti-glycation and anticancer properties of curcumin longa and okra seed extract were studied alone and also in combination with the well-established drug metformin. The combined effect of curcumin with metformin and metformin with okra seed extract was found to be highly efficacious in inhibiting Advanced Glycation End-products （AGEs）. In order to understand the mechanistic implications of curcumin combined with metformin and its enhanced anti-glycation activity, a Curcumin-Metformin Adduct was chemically synthesized. This adduct was fully characterized by thin-layer chromatography, Nano Drop spectrophotometry and electrospray-ionization mass spectrometry. The adduct may be helpful not only in elucidating the mechanism of anti-glycation and anti-cancer activities but also in studying the role of curcumin in binding of AI3-oligomers and disaggregating fibrillar formation in Alzheimer＇s disease.

摘要：Peptide inhibition of the interactions of the tumor suppressor protein P53 with its negative regulators MDM2 and MDMX activates P53 in vitro and in vivo,representing a viable therapeutic strategy for cancer *** phage display techniques,we previously identified a potent peptide activator of P53,termed PMI(TSFAEYWNLLSP),with binding affinities for both MDM2 and MDMX in the low nanomolar concentration *** we report an ultrahigh affinity,dual-specificity peptide antagonist of MDM2 and MDMX obtained through systematic mutational analysis and additivitybased molecular *** assays of over 100 peptide analogs of PMI using surface plasmon resonance and fluorescence polarization techniques yielded a dodecameric peptide termed PMI-M3(LTFLEYWAQLMQ)that bound to MDM2 and MDMX with K_(d)values in the low picomolar concentration range as verified by isothermal titration ***-crystal structures of MDM2 and of MDMX in complex with PMI-M3 were solved at 1.65 and 3.0 A resolution,*** to PMI,PMI-M3 occupied the P53-binding pocket of MDM2/MDMX,which was dominated energetically by intermolecular interactions involving Phe3,Tyr6,Trp7,and Leu *** differences in binding between PMI-M3 and PMI were observed at other positions such as Leu4 and Met11 with MDM2,and Leu1 and Met11 with MDMX,collectively contributing to a significantly enhanced binding affinity of PMI-M3 for both *** adding lysine residues to both ends of PMI and PMI-M3 to improve their cellular uptake,we obtained modified peptides termed PMI-2K(KTSFAEYWNLLSPK)and M3-2K(KLTFLEYWAQLMQK).Compared with PMI-2K,M3-2K exhibited significantly improved antitumor activities in vitro and in vivo in a P53-dependent *** super-strong peptide inhibitor of the P53-MDM2/MDMX interactions may become,in its own right,a powerful lead compound for anticancer drug development,and can aid molecular design of other classes of P53 activators as well for anticancer therapy.

摘要：Developing efficient platinum(Pt)-based electrocatalysts is enormously significant for fuel ***,we report an integrated electrocatalyst of ultralow-Pt alloy encapsulated into nitrogen-doped nanocarbon architecture for efficient oxygen reduction *** hybrid Pt-based catalyst achieves a mass activity of 3.46 A mg^(-1)_(pt)the potential of 0.9 V *** with a negligible stability decay after 10,000 *** importantly,this half-cell activity can be expressed at full cell level with a high Pt utilization of 10.22 W mg^(-1)_(Pt cathode)and remarkable durability after 30,000 cycles in *** and theoretical investigations reveal that a highly strained Pt structure with an optimal Pt-0 binding energy is induced by the incorporation of Co/Ni into Pt lattice,which would account for the improved reaction *** synergistic catalysis due to nitrogen-doped nanocarbon architecture and active Pt component is responsible for the enhanced catalytic ***,the strong metal-support interaction and optimized hydrophilic properties of nanocarbon matrix facilitate efficient mass transport and water *** work may provide significant insights in designing the low-Pt integrated electrocatalysts for fuel cells and beyond.