摘要：In order to evaluate the efficacy of low intensity ultrasound and tissue engineering technique to repair segmental bone defects, the rabbit models of 1.5-cm long rabbit radial segmental osteoperiosteum defects were established and randomly divided into 2 groups. All defects were implanted with the composite of calcium phosphate cement and bone mesenchymal stem cells, and ad- ditionally those in experimental group were subjected to low intensity ultrasound exposure, while those in control group to sham exposure. The animals were killed on the postoperative week 4, 8 and 12 respectively, and specimens were harvested. By using radiography and the methods of biomechanics, histomorphology and bone density detection, new bone formation and material degradation were observed. The results showed that with the prolongation of time after operation, serum alkaline phosphatase （AKP） levels in both groups were gradually increased, especially in experimental group, reached the peak at 6th week （experimental group： 1,26 mmol/L; control group： 0.58 mmol/L）, suggesting the new bone formation in both two group, but the amount of new bone formation was greater and bone repairing capacity stronger in experimental group than in control group. On the 4th week in experimental group, chondrocytes differentiated into woven bone, and on the 12th week, remodeling of new lamellar bone and absorption of the composite material were observed. The mechanical strength of composite material and new born density in experimental group were significantly higher than in control group, indicating that low intensity ultrasound could not only effectively increase the formation of new bone, but also accelerate the calcification of new bone. It was concluded that low intensity ultrasound could evidently accelerate the healing of bone defects repaired by bone tissue engineering.

摘要：Objective： To study the biomechanical feature of a newly designed cervical vertebra internal fixation device and its clinical applications. Methods： Some functional spinal units were fixed respectively with titanium plate, fusion cage and new device designed by ourselves, then a controlled biomechanical study including flexion, extension, torsion and lateral bending was performed and the results were analyzed. Results： As to the mechanical performance, fusion cage showed poor performance in extension test and so did the titanium plate in the distortion test. However, the new device showed good performance in every test. Conclusion： Both simple titanium plate fixation and simple fusion cage fixation have biomechanical defaults, but they are complementary. The titanium plate-interbody fusion cage avoids the defaults and has specific advantages.

摘要：Objective： To study whether the range of knee flexion （ROF） is affected by geometrical mismatch of the femoral component and the resultant change in the pos- terior condylar offset （PCO） after high-flexion posterior-sta- bilized total knee arthroplasty （TKA）. Methods： One hundred osteoarthritic patients （50 males and 50 females） underwent femoral osteotomy by the ante- rior referencing technique. The PCO for each patient was measured from lateral radiographs before, during and 2 years after TKA. The thickness of the joint cartilage was mea- sured by magnetic resonance imaging before TKA and added onto the radiographic measurement. The relationship between changes in the PCO and improvements in the ROF before, during and 2 years after TKA were statistically analyzed. Results： Compared with the preoperative value, the PCO was reduced by （3.45±3.28） mm after TKA, with a significantly larger reduction observed in female patients than male patients （P〈0.05）. When examining the subject popu- lation as a whole, there was a significant positive correla- tion between PCO and ROF improvement during TKA （P〈 0.05）, but this improvement was not maintained 2 years after TKA （P〉0.05）. However, when male and female patients were analyzed separately, there was a significant positive correlation between PCO change and ROF improvement for both sexes at both time points （all P〈0.05）. Conclusions： Restoration of PCO plays an important role in the optimization of knee flexion even after posteriorstabilized TKA. Femoral components based on Caucasian anatomic characteristics could not match the native anatomy of distal femurs in Chinese population especially female Chinese. Rotated resection of distal femur with anterior referencing technique usually leads to a decreased PCO and therefore reduces maximal obtainable flexion.

摘要：Objective: To establish and optimize an enhanced recovery after surgery (ERAS) program, for the classic posterior lumbar decompression and fusion (PLDF).Methods: *** ERAS for PLDF procedure during the perioperative period had been designed. 2.A total of 155 patients (73 in the ERAS group and 82 in the traditional health care group) were analyzed, and their clinical outcomes were compared. The evaluation indexes included physiological function, postoperative visual analogue scale (VAS), pain score, postoperative complications. Results: ERAS significantly promoted early food-taking (7.93±2.15h vs 24.54 ± 5.72h, P < 0.00), early catheter removal (36.31 ± 8.42h vs 71.48 ± 13.75h, P < 0.00), early defecation (3.80 ± 1.3 days vs 5.3±1.41 days, P < 0.00);reduced the incidence of urinary tract infection (2.7% vs 9.7% P = 0.01) and shorter hospital stay (3.80 ± 1.04 days vs 7.29±1.62 days, P < 0.00), while no difference between the two groups in vomiting, lung infection, wound bleeding and infection. Conclusion: ERAS for PLDF can facilitate the recovery of physiological function, reduce postoperative pain, reduce operative complications and morbidity after surgery and contribute to a shorter hospital stay. Further research is needed to optimize the process.

摘要：A good Ti-based joint implant should prevent stress shielding and achieve good bioactivity and anti-infection *** meet these requirements,the low-elastic-modulus alloy—Ti–35Nb–2Ta–3Zr—was used as the substrate,and functional coatings that contained bioceramics and Ag ions were prepared for coating on TiO_(2)nanotubes(diameter:(80±20)nm and(150±40)nm)using anodization,deposition,and spin-coating *** effects of the bioceramics(nano-β-tricalcium phosphate,microhydroxyapatite(micro-HA),and meso-CaSiO_(3))and Ag nanoparticles(size:(50±20)nm)on the antibacterial activity and the tribocorrosion,corrosion,and early in vitro osteogenic behaviors of the nanotubes were *** tribocorrosion and corrosion results showed that the wear rate and corrosive rate were highly dependent on the features of the nanotube ***-HA showed great wear resistance with a wear rate of(1.26±0.06)×10^(−3)mm^(3)/(N·m)due to adhesive and ***-CaSiO_(3)showed enhanced cell adhesion,proliferation,and alkaline phosphatase *** coatings that contained nano-Ag exhibited good antibacterial activity with an antibacterial rate of≥89.5%against Escherichia *** findings indicate that hybrid coatings may have the potential to accelerate osteogenesis.

摘要：Acellular porcine small intestinal submucosa(SIS)has been used for esophagoplasty with success in a canine ***,it did not lead to complete *** better reconstruction,a cellular component is ***,promotion of angiogenesis with copper has been widely recognized by basic research as well as clinical *** this study,we have evaluated the feasibility and effectiveness of combined Cu and SIS(SIS-Cu patch)for the esophageal repair using a canine *** male beagle dogs were subjected to surgical resection to produce cervical esophageal defects(5 cm in length,180°in range).SIS with Cu(5 or 25μmol L 1copper)or without Cu was patched on the esophageal *** esophagram and histology exam were carried out to evaluate the effectiveness of the *** shown,the SIS-Cu graft promoted re-epithelialization,re-vascularization and muscular ***-Cu patch is more effective than SIS alone for esophageal repair,and the SIS+25μmol L 1Cu group demonstrated additional advantages over the SIS+5μmol L 1Cu.

摘要：Intracellular electrophysiological research is vital for biological and medical *** planar microelectrode arrays(MEAs)have disadvantages in recording intracellular action potentials due to the loose cell-electrode *** investigate intracellular electrophysiological signals with high sensitivity,electroporation was used to obtain intracellular *** this study,a biosensing system based on a nanoporous electrode array(NPEA)integrating electrical perforation and signal acquisition was established to dynamically and sensitively record the intracellular potential of cardiomyocytes over a long period of ***,nanoporous electrodes can induce the protrusion of cell membranes and enhance cell-electrode interfacial coupling,thereby facilitating effective *** signals over the entire recording process can be quantitatively and segmentally analyzed according to the signal changes,which can equivalently reflect the dynamic evolution of the electroporated cardiomyocyte *** believe that the low-cost and high-performance nanoporous biosensing platform suggested in this study can dynamically record intracellular action potential,evaluate cardiomyocyte electroporation,and provide a new strategy for investigating cardiology pharmacological science.

摘要：The key to managing fracture is to achieve stable internal fixation,and currently,biologically and mechanically appropriate internal fixation devices are urgently *** excellent biocompatibility and corrosion resistance,titanium–niobium alloys have the potential to become a new generation of internal fixation materials for ***,the role and mechanism of titanium–niobium alloys on promoting fracture healing are still ***,in this study,we systematically evaluated the bone-enabling properties of Ti45Nb via in vivo and in vitro *** vitro,we found that Ti45Nb has an excellent ability to promote MC3T3-E1 cell adhesion and proliferation without obvious *** phosphatase(ALP)activity and alizarin red staining and semiquantitative analysis showed that Ti45Nb enhanced the osteogenic differentiation of MC3T3-E1 cells compared to the Ti6Al4V *** the polymerase chain reaction experiment,the expression of osteogenic genes in the Ti45Nb group,such as ALP,osteopontin(OPN),osteocalcin(OCN),type 1 collagen(Col-1)and runt-related transcription factor-2(Runx2),was significantly higher than that in the control ***,in the western blot experiment,the expression of osteogenic-related proteins in the Ti45Nb group was significantly increased,and the expression of PI3K–Akt-related proteins was also higher,which indicated that Ti45Nb might promote fracture healing by activating the PI3K–Akt signaling *** vivo,we found that Ti45Nb implants accelerated fracture healing compared to Ti6Al4V,and the biosafety of Ti45Nb was confirmed by histological ***,immunohistochemical staining confirmed that Ti45Nb may promote osteogenesis by upregulating the PI3K/Akt signaling *** study demonstrated that Ti45Nb exerts an excellent ability to promote fracture healing as well as enhance osteoblast differentiation by activating the PI3K/Akt signaling pathway,and its good biosafety has been confirmed

摘要：Gradual distraction with an external fixator is a widely used treatment for severe postburn ankle contracture(SPAC).However,application of external fixators is complex,and conventional two-dimensional(2D)imaging-based surgical planning is not particularly helpful due to a lack of spatial *** purpose of this study was to evaluate the surgical planning process for this procedure with patient-specific three-dimension-printed models(3DPMs).In this study,patients coming from two centers were divided into two cohorts(3DPM group *** group)depending on whether a 3DPM was used for preoperative surgical *** duration,improvement in metatarsal-tibial angle(MTA),range of motion(ROM),the American Orthopedic Foot and Ankle Society(AOFAS)scores,complications,and patient-reported satisfaction were compared between two *** 3DPM group had significantly shorter operation duration than the control group((2.0±0.3)h vs.(3.2±0.3)h,P<0.01).MTA,ROM,and AOFAS scores between the two groups showed no significant differences pre-operation,after the removal of the external fixator,or at *** feet were achieved and gait was substantially improved in all patients at the final ***-tract infections occurred in two patients(one in each group)during distraction and were treated with wound care and oral *** in the 3DPM group reported higher satisfaction than those in the control group,owing to better patient-surgeon *** planning using patient-specific 3DPMs significantly reduced operation duration and increased patient satisfaction,while providing similar improvements in ankle movement and function compared to traditional surgical planning for the correction of SPAC with external fixators.

摘要：Objective: To repair rabbit tendon defects with tissue engineering method. Methods: The third passage of fetal skin fibroblast cells was labeled with 5 bromo 2 deoxyuridine (Brdu) and then seeded on human amnion extracellular matrix ( HA ECM ). Using 1 cm long Achilles tendon defects as repairing models in the experimental group, tendon defects were core bridged with polydioxanone (PDS) and then capsulated with the complex of fibroblasts HA ECM . In the control group I, defective tendons were sutured with PDS following the former procedure and capsulated with HA ECM (without fibroblasts). In the control group II, only PDS was applied to connect the defective tendons. Gross examination, light microscopy, scanning electronmicroscopy and biomechanical measurement of the repaired tendons were respectively performed at postoperative 1, 2, 3 month as well as immunohistochemical examination. Results: The optimal cell concentration for seeding fibroblasts was 3.5 ×10 6 cells/ml. Cells grew well and radiated or paralleled on HA ECM . Immunohistochemistry showed that the labeled seed fibroblasts played an important role in tendonization. The results of light microscopy, electron microscopy, and biomechanical assessment suggested that the rate and quality of tendonization in the experimental group was superior to those of the control group I and II. The tensile strength in the experimental group was the greatest, the next was in the control group I, and the worst in the control group II (P< 0.05 ). Conclusions: HA ECM is the excellent carrier for fibroblasts. Fibroblasts HA ECM complex has the capability to repair tendon defect and to tendonize with rapid rate and good performance three months after operation. Its tensile strength is 81.8 % of that of normal tendon.