摘要：Designing regional pattern is challenging for ecosystem restoration both in theory and in practice. The present study proposed a new approach to design regional pattern for ecosystem restoration with a case in the arid valley of Upper Minjiang River, SW China. The procedures are: (1) Determine the regional pattern of main environmental factors based on the relationships between the important environmental factors and the main geographical factors generated from DEM data. (2) Overlay the maps of the main environmental factors. (3) Generate a lookup table according to the species selection rule we proposed to link the candidate restoration species to the main environmental factors.(4) Determine the regional pattern of ecosystem restoration by positioning the restoration species in the map of environmental factors according to the lookup table. The regional restoration pattern designed in this way would be applicable not only to restoring a region, but also to predicting the future landscape change.

摘要：An effective evaluation system can provide specific and practical suggestions to the deep groundwater management. But such kind of evaluation system has not been proposed in China. In this study, an evaluation index system is specifically developed to evaluate deep groundwater management efficiency. It is composed of three first-level indicators(law enforcement capability, management ability, and management effectiveness) and eleven second-level indicators. The second-level indicators include seven mandatory indicators and four optional indicators. Piecewise linear function is used to normalize the quantitative indicators, and expert scoring method and questionnaire survey method are used to normalize the qualitative indicators. Then a comprehensive indicator weighting evaluation method is used to evaluate the first-level indicators and the target topic. A case study is carried out to evaluate deep groundwater management efficiency in Tianjin City. According to the evaluation score in each period, the management efficiency of every district in Tianjin City gradually improved. The overall evaluation score in the early deep groundwater extraction period is 0.12. After a series of deep groundwater protection efforts, this score reached to 0.61 in 2007, and met the regulation criteria. The evaluation results also showed that the further groundwater management efforts in Tianjin City should be focused on building a dynamic database to collect comprehensive deep well-log data; and on a reasonable design and distribution of the groundwater monitoring network. It demonstrated that the index system is suitable to locate the deficiencies of current groundwater management systems and to guide further improvements. It can then be used to protect deep groundwater.

摘要：The Activated Sludge Process(ASP) exhibits highly nonlinear properties. The design of an automatic control system that is robust against disturbance of inlet wastewater flow rate and has short process settling times is a challenging matter. The proposed control method is an I-P modi fied controller automatic control system with state variable feedback and control canonical form simulation diagram for the process. A more stable response is achieved with this type of modern control. Settling times of 0.48 days are achieved for the concentration of microorganisms,(reference value step increase of 50 mg·L-1) and 0.01 days for the concentration of oxygen(reference value step increase of 0.1 mg·L-1). Fluctuations of concentrations of oxygen and microorganisms after an inlet disturbance of5 × 103m3·d-1are small. Changes in the reference values of oxygen and microorganisms(increases by 10%, 20% and 30%) show satisfactory response of the system in all cases. Changes in the value of inlet wastewater flow rate disturbance(increases by 10%, 25%, 50% and 100%) are stabilized by the control system in short time. Maximum percent overshoot is also taken in consideration in all cases and the largest value is 25% which is acceptable. The proposed method with I-P controller is better for disturbance rejection and process settling times compared to the same method using PI controller. This method can substitute optimal control systems in ASP.

摘要：Photodynamic therapy(PDT)has significant advantages in treating primary ***,the hypoxic tumor microenvironment hinders the generation of sufficient reactive oxygen species during PDT to effectively kill tumor cells,further greatly limiting the applications of PDT in cancer ***,we reported a temperature/pH dual controlled drug delivery system LPC@PCN@PDA/Fe^(3+)-AS1411 based on a porous coordination network(PCN(Mn))coated with polydopamine(PDA)and modified with an aptamer AS1411.β-lapachone(LPC)was loaded inside the PCN(Mn)framework,and Fe^(3+)was attached to the surface of the PDA *** nanoparticles(NPs)exhibited excellent multimodal cancer therapeutic effects and tumor targeting ability with their photo-and chemodynamic *** therapeutic effect can be enhanced by the production of sufficient oxygen by the internal hydrogen peroxide,which improves the photodynamic effect of the photosensitizer PCN(Mn)and the chemotherapy effect ofβ-***,the conversion of Fe^(2+)to Fe^(3+)in the tumor cells exerts the Fenton effect,which generates hydroxyl radicals that cause lipid peroxidation in tumor cells and induce apoptosis,thus enhancing the chemodynamic therapeutic *** vitro and in vivo experiments revealed that the NPs demonstrated specific tumor targeting,excellent inhibition effect on tumor growth,and ***,our findings can help develop an intelligent multifunctional therapeutic nanoplatform for cancer therapy.

摘要：The denitrifier method is widely used as a novel pretreatment method for the determination of nitrogen and oxygen isotope ratios as it can provide quantitative and high-sensitivity measurements. Nevertheless, the method is limited by relatively low measurement accuracy for δ18 O. In this study, we analyzed the factors influencing the accuracy of δ18 O determination, and then systematically investigated the effects of dissolved oxygen concentrations and nitrate sample sizes on estimates of the δ15 N and δ18 O of nitrate reference materials. The δ18 O contraction ratio was used to represent the relationship between the measured difference and true difference between two reference materials. We obtained the following main results:(1) a gas-liquid ratio of 3:10(v/v) in ordinary triangular flasks and a shaking speed of 120 r/min produced an optimal range(1.9 to 2.6 mg/L) in the concentration of dissolved oxygen for accurately determining δ18 O, and(2) the δ18 O contraction ratio decreased as nitrate sample size decreased within a certain range(1.0 to 0.1 μmol). Our results suggested that δ18 O contraction is influenced mainly by dissolved oxygen concentrations in pure culture, and provided a model for improving the accuracy of oxygen isotope analysis.

摘要：This paper examines the capability of three regional climate models (RCMs), i.e., RegCM3 (the International Centre for Theoretical Physics Regional Climate Model), PRECIS (Providing Regional Climates for Impacts Studies) and CMM5 (the fifth-generation Pennsylvania State University-the National Center for Atmospheric Research of USA, NCAR Mesoscale Model) to simulate the near-surface-layer winds (10 m above surface) all over China in the late 20th century. Results suggest that like global climate models (GCMs), these RCMs have the certain capability of imitating the distribution of mean wind speed and fail to simulate the greatly weakening wind trends for the past 50 years in the country. However, RCMs especially RegCM3 have the better capability than that of GCMs to simulate the distribution and change feature of mean wind speed. In view of their merits, these RCMs were used to project the variability of near-surface-layer winds over China for the 21st century. The results show that 1) summer mean wind speed for 2020-2029 will be lower compared to those in 1990-1999 in most area of China; 2) annual and winter mean wind speed for 2081-2100 will be lower than those of 1971-1990 in the whole China; and 3) the changes of summer mean wind speed for 2081-2100 are uncertain. As a result, although climate models are absolutely necessary for projecting climate change to come, there are great uncertainties in projections, especially for wind speed, and these issues need to be further explored.

摘要：Keeping beef cattle outdoors during winter reduces costs and improves animal welfare, but increases the risk of nitrogen (N) and phosphorus (P) runoff losses. This study evaluated a rotational pen design on grassland with two groups of suckler cows given access to an expanding staying area and a new feeding area each week (72 cattle ha-1), with one month’s stay per pen. The spatial distribution of excreta and effects on N and P surface runoff was evaluated during six months. The total excreta loads corresponded to 500 kg·N·ha-1 and 50 kg·P·ha-1. New feeding areas did not distribute excretions evenly, which resulted in the highest proportion of excretions (31%) occurring in the first week’s sub-area. The topsoil had significantly higher amounts of mineral-N, mainly as NH4-N (29 - 81 kg·ha-1), than an unaffected area (13 kg·ha-1). Mean total runoff losses were similar for both groups (1.4 kg·P·ha-1 and 9.0 kg·N·ha-1). Around 78% of N and 70% of P runoff losses occurred during the month with cattle present. During the first two weeks with heavy rain, N and P runoff losses were 50% higher from an area with suckler cows than a corresponding vegetated sub-area without cows. The study design did not provide a sufficient distribution of excretions and a high animal density in combination with trampling resulted in unacceptable N and P run-off losses. An environmentally friendly design would need to include frequent moving of all equipment and access to larger areas.

摘要：A novel horizontal trap-door test system was devised in this study to analyze the face stability of shield tunnels in *** test system can be used to investigate both the longitudinal and cross sections of the face failure simultaneously at one single apparatus and was employed to perform face stability tests on small-scaled tunnel models at single *** lateral support pressures and failure zones were studied with varying sand materials and earth *** results demonstrate that the tunnel face moves back,the lateral active earth pressure on the tunnel face decreases rapidly to a residual value,and the lateral pressure distribution can be categorized into three stages during the failure process:1)initial state;2)pressure dissipation stage;and 3)pressure zone diminution ***,face failure firstly develops from a stable condition to the local failure state,and then continues to develop to the global failure state that can be divided into two sub-zones with different failure mechanisms:rotational failure zone(lower zone)and gravitational failure zone(upper zone).Further discussion shows that under the effects of soil arching,the shape of the gravitational failure zone can adopt arch shaped(most frequent)and column shaped(in shallow tunnels).Limit support pressure for face stability usually appears atδ/D=0.2%−0.5%(ratio of face displacement to tunnel diameter).

摘要：多模态成像引导的光动力和光热治疗在癌症治疗上具有明显的优势.同时具有近红外光(NIR)吸收、高活性氧(ROS)产率和高光热转换效率的物质是非常理想的光疗试剂.本文设计合成了一种具有“受体-供体-受体”(A-D-A)结构的分子IDCIC.随后通过在IDCIC表面包裹DSPE-PEG2000-NH2得到了水溶性纳米颗粒IDCIC *** NPs具有近红外吸收,峰值位于760 nm;同时还具有近红外二区荧光发射,峰值在1000 nm左右,荧光量子产率为1.2%,使得IDCIC NPs具有良好的光声成像和NIR-Ⅱ荧光成像能力.此外,IDCIC NPs在808 nm激光器照射下可以同时产生单线态氧(量子产率为9.1%)、羟基自由基(·OH)和热量(光热转换效率为78.9%).基于以上这些特性,IDCIC NPs可以用于多模态成像引导的光动力/光热联合癌症治疗.

摘要：前期研究表明梭菌属的新株JC3(*** JC3)是嗜热产甲烷污泥中的主要纤维素降解菌。本项目采用分子生物学技术定量研究了菌株JC3在高温厌氧消化过程中的作用。在含三个小室(室1,室2,室3)的厌氧折流板反应器(9.5 L)中进行纤维素降解实验,试验温度设为55℃。当负荷为2.5 kg COD.m-3d-1,HRT为2天时,80%以上的COD进料都转化为甲烷。采用专门针对菌株JC3的特异性荧光针来研究折流式反应器中污泥样品。结果表明JC3细胞对DAPI染色细胞的比例从低于0.5%(最低检测极限)增加到9.4%(室1),13.1%(室2)和21.6%(室3)。这表明由荧光原位杂交技术确定的菌株JC3细胞数与滞留污泥的纤维素降解产甲烷活性密切相关。设计了一个针对菌株JC3纤维素酶基因(纤维二糖水解酶A cbh A)的特异探针,并应用到处理固体废弃物如咖啡渣、废纸、垃圾和纤维素等消化污泥中,采用定量PCR技术发现菌株JC3细胞数与高温消化器中污泥的纤维素降解能力密切相关。因此,在高温厌氧消化过程中,菌株JC3对厌氧水解纤维素是非常重要的。