摘要：Abstract Meniscus injury is a common disease in *** it was not treated in time,it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the *** present,meniscectomy and meniscus suture are widely used in the treatment for meniscus ***,It is not ideal for poor self-healing ability of *** recruitment of endogenous stem cells is an attractive option for wounded meniscus *** reduced high-mobility group box 1 protein(HMGB1)can accelerate the regeneration of multiple tissues by endogenous stem cell activation,migration and ***(KGN)has shown to induce the chondrogenesis of the stem ***,no study has explored such effects of HMGB1 and KGN in wounded meniscus ***,in order to improve the regeneration of meniscus,we intend to use a novel bioactive microsphere which was developed by combining fully reduced high mobility group box 1(frHMGB1)and kartogenin(KGN)with alginate gel which slowly release high concentrations of HMGB1 and KGN to activate rat bone marrow stem cells(BMSCs)and promote cell *** results showed that this HMGB1–KGN microsphere released and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 *** vitro experimental results showed that the HMGB1–KGN microsphere can promote cell proliferation via recruiting rat bone marrow stem cells(BMSCs)and activating the BMSCs from G_(0) to G_(Alert) stage as evidenced by cell migration testing and 5-bromo-2′-deoxyuridine(BrdU)incorporation *** vivo results indicated that this HMGB-KGN microsphere can recruit GFP-labeled BMSCs from tail vein to wounded meniscus and induce these GFP-labeled BMSCs to differentiate into *** results demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus healing in vivo.

摘要：Oxidized Bacterial Cellulose （OBC）-hydroxyapatite （HAp）-gelatin （Gel） nanocomposites were prepared by a biomimetic process. HAp nanocrystals were precipitated in a mixed solution ofNa2HPO4 （pH 9.2） and Gel solution at 37 ℃, and OBC was used to generate a three-dimensional （3D） network stent. The tensile strength of OBC-HAp-G was higher than 0.3 MPa, and the complete degradation time was approximately 90 d in Simulated Body Fluid （SBF）. Fourier transform infrared spectroscopy demonstrated that a coordinate bond had formed possibly between HAp and the cellulose hydroxyl. X-ray diffraction showed that both the oxidation of bacterial cellulose and an increase in Gel content induced the formation of tiny HAp crystallites during composite fabrication. Specific surface area and porosity measurements indicated that a low Gel concentration contributed to retention of porous structure. The Ca and P contents on the surface of materials increased initially and then decreased with an increase in Gel content, as measured by energy dispersive spectroscopy. From the thermogravimetric data, the increase in decomposition temperature suggested the formation of chemical bonds among OBC, HAp, and Gel. The above results suggest that the OBC-HAp-G0.3 composite is a potential bone scaffold material.

摘要：Background:Numerous studies about the interaction between footwear(and barefoot) and kinematic and kinetic outcomes have been published over the last few *** studies however lead to the conclusion that the assumed interactions depend mainly on the subjects" experience of barefoot(BF) walking/running,the preferred running strike pattern,the speed,the hardness of the surface,the thickness of the midsole material,and the runners’ level of *** aim of the present study was to investigate lower leg kinematics o\’ BF running and running in minimal running shoes(MRS) to assess comparability of BF kinematics in both *** systematically compare both conditions we monitored the influencing variables described above in our measurement *** hypothesized that running in MRS does not alter lower leg kinematics compared to BF ***:Thirty-seven subjects,injury-free and active in sports,ran BF on an EVA foam runway,and also ran shod wearing Nike Free 3.0 on a tartan indoor ***-leg 3D kinematics was measured to quantify rearfoot and ankle *** markers were used in both shod and BF ***:All runners revealed rearfoot strike pattern when running *** between BF and MRS running occurred particularly during the initial stance phase of running,both in the sagittal and the frontal *** running revealed a flatter foot placement,a more plantar flexed ankle joint and less inverted rearfoot at touchdown compared to MRS ***:BF running does not change the landing automatically to forefoot running,especially after a systematic exclusion of surface and other influencing *** Nike Free 3.0 mimics some BF ***,changes in design of the Nike Free should be considered in order to mimic BF movement even more closely.

摘要：Existing technologies used to detect monosodium urate(MSU)crystals for gout diagnosis are not ideal due to their low sensitivity and complexity of *** purpose of this study was to explore whether aggregation-induced emission luminogens(AIEgens)can be used for highly specific imaging of MSU crystals to assist in the diagnosis of ***,we developed a series of luminogens(i.e.,tetraphenyl ethylene(TPE)-NH_(2),TPE-2NH_(2),TPE-4NH_(2),TPE-COOH,TPE-2COOH,TPE-4COOH,and TPE-Ketoalkyne),each of which was then evenly mixed with MSU ***,optimal fluorescence imaging of each of the luminogens was characterized by a confocal laser scanning microscope(CLSM).This approach was used for imaging standard samples of MSU,hydroxyapatite(HAP)crystals,and mixed samples with 1:1 mass ratio of MSU/*** also imaged samples from mouse models of acute gouty arthritis,HAP deposition disease,and comorbidities of ***,CLSM imaging results were compared with those of compensated polarized light microscopy,and we assessed the biosafety of TPE-Ketoalkyne in the RAW264.7 cell ***,CLSM time series and three-dimensional imaging were performed on MSU crystal samples from human gouty synovial fluid and *** a promising candidate for MSU crystal labeling,TPE-Ketoalkyne was found to detect MSU crystals accurately and rapidly in standard samples,animal samples,and human samples,and could precisely distinguish gout from HAP deposition *** work demonstrates that TPE-Ketoalkyne is suitable for highly specific and timely imaging of MSU crystals in gouty arthritis and may facilitate future research on MSU crystal-related diseases.

摘要：The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie