摘要：In an industrialized world, virtually everything we are using is first designed and then manufactured. Let us use the automobile manufacturing industry as an example: engineers design vehicles based on laws of physics;select proper materials for machine parts;and assemble parts following blueprints into cars, buses, and trucks. It is evident that we need not only parts but also technical drawings and instructions for the assembly of parts into a functional pump or clutch.

摘要：Increasing evidence indicates that engineered nerve grafts have great potential for the regeneration of peripheral nerve injuries(PNIs).While most studies have focused only on the topographical features of the grafts,we have considered both the biophysical and biochemical manipulations in our applied *** achieve this,we fabricated an electrospun nanofibrous scaffold(ENS)containing polylactide nanofibers loaded with lithium(Li)ions,a Wnt/β-catenin signaling *** addition,we seeded human adipose-derived mesenchymal stem cells(hADMSCs)onto this engineered scaffold to examine if their differentiation toward Schwann-like cells was *** further examined the efficacy of the scaffolds for nerve regeneration in vivo via grafting in a PNI rat *** results showed that Li-loaded ENSs gradually released Li within 11 d,at concentrations ranging from 0.02 to(3.64±0.10)mmol/L,and upregulated the expression of Wnt/β-catenin target genes(cyclinD1 and c-Myc)as well as those of Schwann cell markers(growth-associated protein 43(GAP43),S100 calcium binding protein B(S100B),glial fibrillary acidic protein(GFAP),and SRY-box transcription factor 10(SOX10))in differentiated *** the PNI rat model,implantation of Li-loaded ENSs with/without cells improved behavioral features such as sensory and motor functions as well as the electrophysiological characteristics of the injured *** improved function was further validated by histological analysis of sciatic nerves grafted with Li-loaded ENSs,which showed no fibrous connective tissue but enhanced organized myelinated *** potential of Li-loaded ENSs in promoting Schwann cell differentiation of hADMSCs and axonal regeneration of injured sciatic nerves suggests their potential for application in peripheral nerve tissue engineering.

摘要：Recently, engineered minichromosomes have been produced using a telomere-mediated truncation technique in some plants. However, the study on transferring genes to minichromosomes is very ***, telomere-mediated truncation was successfully performed in common wheat（Triticum aestivum）to generate stable truncated chromosomes accompanied by a relatively high frequency of chromosomal rearrangements. After the cross between transgenic parents, a promoter-less DsRed gene in a chromosome from one parent was transferred to another chromosome from the other parent at the site behind a maize ubiquitin promoter via the Cre/lox system. DsRed transcripts and red fluorescent proteins were detected in the recombinant plants. In one such seedling, transgenic signals were detected at the centric terminus of chromosome 4D and the distal terminus of chromosome 3A. Clear translocations could be detected at the transgenic loci of these two chromosomes. Intriguingly, signals of centric-specific sequences were co-localized with the translocated D-group chromosomal segment in the terminal region of chromosome 3A. Our results indicate that the Cre/lox system induces the gene swapping to the target chromosome and non-homologous chromosomal recombination simultaneously. These approaches could offer a platform to transfer large DNA fragments or even terminal chromosomal segments to other chromosomes of the natural genome.

摘要：Photosynthesis is the basis of plant productivity. There is still a big gap between the efficiency of theoretical maximal photosyn- thetic energy conversion and the efficiency realized in the field. It was estimated that for C3 plants, the theoretical maximal energy conversion efficiency is ca. 4.6%, the observed maximal season- long energy conversion efficiency is about 2.4%, while the typical season-long energy conversion efficiency is only 0.8% （Zhu et al., 2010）. Despite this, however, photosynthesis has not been effectively used as a breeding target for crop improvement so far. When plants grow under elevated CO2, the crop yields are higher, clearly showing that enhancing photosynthesis can be used to improve yields （Long et al., 2006; Zhu et al., 2010）. Two reasons underlie the failure of improving photosynthesis for greater yields. First, photosynthesis is difficult to phenotype compared with other traits such as flowering time, plant height, leaf erectness, etc.; second, canopy photosynthesis, instead of leaf photosynthesis, is related to crop biomass and yield, however, there is no effective way to measure canopy photosynthesis and to identify the factors controlling canopy photosynthetic energy conversion efficiency.

摘要：Plants are known to be efficient hosts for the production of mammalian therapeutic ***,plants produce complex N-glycans bearingβ1,2-xylose and coreα1,3-fucose residues,which are absent in *** immunogenicity and allergenicity of plant-specific Nglycans is a key concern in mammalian *** this study,we amplified the sequences of 2 plant-specific glycosyltransferases from Nicotiana tabacum *** Bright Yellow 2(BY2),which is a well-established cell line widely used for the expression of therapeutic *** expression of the endogenous xylosyltranferase(XylT)and fucosyltransferase(FucT)was downregulated by using RNA interference(RNAi)*** xylosylated and core fucosylated N-glycans were significantly,but not completely,reduced in the glycoengineered ***,these RNAi-treated cell lines were stable and viable and did not exhibit any obvious ***,this study may provide an effective and promising strategy to produce recombinant glycoproteins in BY2 cells with humanized N-glycoforms to avoid potential immunogenicity.

摘要：Rice is one of the most important staple food for over half of the world＇s population,and a substantial increase in productivity and quality of rice grain will be required to feed a growing human *** size and shape are the two important components contributing to grain yield and quality,because they impact both yield potential and end-use quality.

摘要：Multicellular microtissues of primary human hepatocytes(PHHs)co-cultured with other supporting cell types are a promis-ing model for drug screening and toxicological ***,these liver microtissues(LMs)rapidly lose their functions during ex vivo ***,in order to mimic the cellular and structural hepatic microenvironment,we co-cultured PHHs with human mesenchymal stromal cells(MSCs)and human umbilical vein endothelial cells(HUVECs)in the presence of cell-sized microparticles(MPs)derived from liver extracellular matrix(LEMPs).The microwell culture platform enabled biofabrication of size-controlled multicellular microtissues(PHH:HUVEC:MSC=3:2:1)with efficient LEMP incorporation(about 70%at a 2:1 ratio of cells:MP).The biofabricated liver microtissues(BLMs)were cultured ex vivo for 14 days and compared to the cell-only LM in terms of gene and protein expression,functional activity,cytochrome P450(CYP450)enzyme inducibility,and drug *** results supported superior hepatic-related gene expression,functional activity,and polarity for PHH in BLM compared to ***450 enzyme inducibility and dose-responsive sensitivity to toxic drugs were significantly higher in the BLM *** conclusion,microtissue engineering by incorporation of tissue-specific microparticles within a multicellular microtissue can offer some advantages for drug discovery studies and cell transplantation *** the near future,this approach could generate a scalable platform of several functional biofabricated microtissues representing different organs.