摘要：Autograft or metal implants are routinely used in skeletal ***,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering *** use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-dimensional(3D)bioprintingof skeletal tissueis anattractivestrategyfor ***,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic *** this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based *** inclusion of the nanofiller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon *** composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)*** inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven *** study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

摘要：Additive manufacturing(AM)has revolutionized the design and manufacturing of patient-specific,three-dimensional(3D),complex porous structures known as scaffolds for tissue engineering *** use of advanced image acquisition techniques,image processing,and computer-aided design methods has enabled the precise design and additive manufacturing of anatomically correct and patient-specific implants and ***,these sophisticated techniques can be timeconsuming,labor-intensive,and ***,the necessary imaging and manufacturing equipment may not be readily available when urgent treatment is needed for trauma *** this study,a novel design and AM methods are proposed for the development of modular and customizable scaffold blocks that can be adapted to fit the bone defect area of a *** modular scaffold blocks can be combined to quickly form any patient-specific scaffold directly from two-dimensional(2D)medical images when the surgeon lacks access to a 3D printer or cannot wait for lengthy 3D imaging,modeling,and 3D printing during *** proposed method begins with developing a bone surface-modeling algorithm that reconstructs a model of the patient’s bone from 2D medical image measurements without the need for expensive 3D medical imaging or *** algorithm can generate both patient-specific and average bone ***,a biomimetic continuous path planning method is developed for the additive manufacturing of scaffolds,allowing porous scaffold blocks with the desired biomechanical properties to be manufactured directly from 2D data or *** algorithms are implemented,and the designed scaffold blocks are 3D printed using an extrusion-based AM *** and instructions are also provided to assist surgeons in assembling scaffold blocks for the self-repair of patient-specific large bone defects.

摘要：目的:评价一种中药植物鞭檐犁头尖(***)作为抗癌药物的临床前研究的系统综述。方法:对Scopus、PubMed、Web of Science、ScienceDirect、LILACS、EBSCO Medline、Mendeley等7个数据库进行全面检索,检索时间截止到2023年9月8日。排除了临床试验和非原创的同行评议报告后,鞭檐犁头尖的提取物,组成,或分离化合物的体内外研究且经过同行评审的英文文献均被纳入。本研究定性评估了鞭檐犁头尖对不同肿瘤细胞、肿瘤大小和作用机制的有效性。证据质量由三名独立评估人员使用ToxRTool进行评估,并验证其一致性。结果:检索后共纳入27项研究:22项体外研究,4项体内研究,1项体内外研究。结果发现鞭檐犁头尖对白血病、乳腺癌、结直肠癌和肺癌有效。大多数研究的评估为“有限制的可靠”。鞭檐犁头尖通过阻断细胞周期、活化caspase-3/-9、分解PARP、裂解DNA、减少生存素含量、降低ROS表达、抑制COX-2和HSP70表达和抑制NF-kB通路等来诱导细胞凋亡。当与干扰素联合使用时,其能阻碍血管生成。结论:尽管鞭檐犁头尖具有良好的抗癌活性,但其作为单独抗癌药物的疗效尚不确定。它似乎更适合用于癌症的补充或联合治疗。研究设计不理想,报告不完整,以及在多篇文章中没有纳入适当的阳性对照和统计分析可能是本研究缺乏结论性证据的原因。

摘要：Gatifloxacin eye drops are frequently used in eye *** such formulations have a major drawback *** duration of action and usually require 4e6 times installations daily.A chitosan coated niosomal formulation of gatifloxain was purposed to show a longer retention time on eyes and subsequent reduction in dosing *** were prepared by solvent injection method using cholesterol and *** extensive optimization of formulation was done using different ratios of cholesterol,Span-60 and drug,revealed NS60-5(cholesterol:span-6050:50 and drug content of 20 mg)to be the optimized niosome ***60-5 had shown a highest entrapment efficiency of 64.9±0.66%with particle size 213.2±1.5 nm and zeta potential
34.7±2.2 *** niosomes were also coated with different concentrations of chitosan and *** studies had revealed that optimized niosomes(86.77±1.31%)had increased the transcorneal permeation of Gatifloxacin more than two fold than simple drug solution(37.19±1.1%).Longer retention potential of the coated niosomes was further verified by fluorescence *** revealed that simple dye solution got easily washed out with in 6 *** uncoated niosomes(NS60-5)showed a longer retention(more than 6 h),which was further enhanced in case of coated niosomes ***60-1(more than 12 h).Antimicrobial studies had shown the better efficacy of CNS60-1(zone of inhibition)when compared to marketed *** final chitosan formulation was found to have shown better ocular tolerability as demonstrated by corneal hydration test histopathology investigations.

摘要：Objective: To optimize the ionic liquid based microwave-assisted extraction(IL-MAE)of polyphenolic content from Peperomia pellucida(L) ***: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model(multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response ***: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concentration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate([bmim]BF_4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X_1+ 2.655X_2+ 2.252X_3– 0.565X_4+ 0.990 X_1X_3– 8.172X_1X_4– 3.439 X_3X_4– 4.178 X_1~2– 3.210 X_3~2– 6.786 X_4~2– 7.290 X_1~2X_3+ 5.575X1_X_3~2– 4.843 X_3~2X_4 with R^2= 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.172 5 mg GAE/***: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.

摘要：Since the start of the Precision Medicine Initiative by the United States of America in 2015,interest in personalized medicine has grown *** short,personalized medicine is a term that describes medical treatment that is tuned to the *** possible way to realize personalized medicine is 3D *** using materials that can be tuned upon stimulation,4D printing is *** recent years,many studies have been exploring a new field that combines 3D and 4D printing with *** has resulted in many concepts of pharmaceutical devices and formulations that can be printed and,possibly,tailored to an ***,the first 3D printed drug,Spritam®,has already found its way to the *** review gives an overview of various 3D and 4D printing techniques and their applications in the pharmaceutical field as drug delivery systems and personalized medicine.

摘要：在过去的年，重要努力被奉献了为同时的治疗和诊断探索新奇的药交货和察觉策略。为人的 hepatocellular 癌房间的荧光察觉，为指向的药和 plasmid 交货的 theranostic nanocarriers 此处被描述的 biotinylated 聚氨酯脲 nanoparticles 的发展。这些指向的 nanoparticles 明确地被设计把 biotin 合并到聚合矩阵，自从许多肿瘤作为增加不受管束的细胞生长的机制为 biotin 打 overexpress 受体。获得的 nanoparticles 是球形的，展出了变化的一条平均直径 110145 nm，并且没在健康 endothelial 房间显示出 cytotoxicity。Biotinylated nanoparticles 有选择地被合并到 perinuclear 和人的 hepatocellular 癌房间线的原子区域， HepG2 在分割，然而并非进成长，健康、人的 endothelial 房间。确实， anticancer 药， phenoxodiol 或 sunitinib 的同时的加入，和编码绿色的 plasmid DNA 荧光灯蛋白质，进这些， nanoparticles 允许指向的药理学 antitumor 效果并且而且，记者基因的选择 transfection，检测这些癌症房间。这里描述的联合指向的治疗和察觉策略能为肝癌症治疗和诊断被利用，与中等安全侧面，并且可以也是为癌症的另外的类型的一个潜在的工具。

摘要：目的调查护士对有喂养困难的唇腭裂患儿所采用的临床奶瓶喂养技术现状。方法采用质性描述性研究设计。于2021年12月至2022年1月,向日本1109所设有产科、新生儿科或小儿牙科病房的医院分别发放匿名问卷。参与者是具有5年以上唇腭裂患儿护理工作经验的护士。问卷包括喂养技术4个方面的开放式问题,包括:奶瓶喂养前的准备、乳头插入方法、吸吮帮助和停止奶瓶喂养的标准。根据意义相似性对资料进行分类和分析。结果共获得410份有效问卷。资料分析结果显示,奶瓶喂养前的准备工作分为7个类别(如,改善患儿的口腔运动、保持患儿平稳呼吸),27个子类别;在乳头插入方法中,分为4个类别(如,用乳头在口腔内形成负压封闭裂口、插入乳头时不接触裂口),11个子类别;在吸吮帮助中,分为5个类别(如,促进觉醒、口腔产生负压),13个子类别;在停止奶瓶喂养的标准中,分为5个类别(如,觉醒水平降低、生命体征变差),16个子类别。96.0%护士希望学习唇腭裂患儿的奶瓶喂养技术。结论本研究确定了多项用于唇腭裂喂养困难患儿的奶瓶喂养技术。然而,这些技术之间存在相互矛盾,方法的有效性也未得到评估。未来需要进行干预研究,以确定各项技术的益处或潜在的危害。

摘要：目的Bouchardatine(1)是从植物Bouchardatia neurococca中分离得到的一种β-吲哚喹唑啉生物碱,可作为脂肪生成的一种调节剂及一种抗癌药。天然产物作为蛋白腺苷5'-单磷酸(AMP)活化蛋白激酶(AMPK)和组蛋白去乙酰化酶1(SIRT1)的激活剂发挥作用。我们利用分子模型研究了化合物1和各种结构类似物的SIRT1结合能力,如从药用植物Oriciarenieri中分离得到的orirenierine A(2)和orirenierine B(3)。方法我们研究了包括β-吲哚喹唑啉生物碱1−3和类似物在内的25种天然产物与人源组蛋白去乙酰化酶1(hSIRT1)的结合,并与参比产物去乙酰化酶(R和S异构体)进行了比较。从hSIRT1催化结构域的闭合和开放状态构象(PDB结构:4KXQ和4IG9)开始阐述去乙酰化酶结合模型。对于与SIRT1结合的每种化合物,我们计算了相互作用的经验能量(ΔE),并与去乙酰化酶进行比较。结果在我们的模型中,发现化合物1与SIRT1的去乙酰化酶位点适度结合。相反,喹唑啉酮部分7位酚羟基表现出更高的结合能力。化合物2提供的SIRT1蛋白复合物与去乙酰化酶观察到的一样稳定。用甲氧基(3)取代羟基取代基(2)降低了SIRT1的结合能力。我们还鉴定了其他SIRT1结合的天然产物,如生物碱orisuaveolines A和B,并讨论了结构结合关系。结论本研究强调了β-吲哚喹唑啉生物碱与SIRT1相互作用的能力。这种去乙酰化酶可以代表生物碱2的分子靶标。这种化合物在设计对SIRT1依赖性病理有活性的药物研究方面值得进一步关注。

摘要：New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements including selectivity, stability, linearity, precision and accuracy. Chromatography was carried out using a LiChrospher RP-18 column, a mixture containing acetonitrile, phosphate buffer of pH 3.5 and methanol (45:45:10, v/v/v) and new fluorescence detection at 255 nm for excitation and 448 nm for emission. The effect of methanol content, pH of the buffer, flow rate, detection wavelengths and column temperature was estimated in robustness study, according to a plan defined by the Plackett-Burman design. For identification of significant effects, both graphical and statistical methods were used. Ro-bustness for dissolution test was checked estimating the effects of paddle speed, temperature and pH of dissolution medium. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of amlodipine and valsartan in their binary mixtures for different analytical and pharmaceutical purposes.