摘要：Engineered sequence-specific zinc finger nucleases (ZFNs) make the highly efficient modification of eukaryotic genomes ***,most current strategies for developing zinc finger nucleases with customized sequence specificities require the construction of numerous tandem arrays of zinc finger proteins (ZFPs),and subsequent largescale in vitro validation of their DNA binding affinities and specificities via bacterial *** labor and expertise required in this complex process limits the broad adoption of ZFN *** effective computational assisted design strategy will lower the complexity of the production of a pair of functional *** we used the FoldX force field to build 3D models of 420 ZFP-DNA complexes based on zinc finger arrays developed by the Zinc Finger Consortium using OPEN (oligomerized pool engineering).Using nonlinear and linear regression analysis,we found that the calculated protein-DNA binding energy in a modeled ZFP-DNA complex strongly correlates to the failure rate of the zinc finger array to show significant ZFN activity in human *** our models,less than 5% of the three-finger arrays with calculated protein-DNA binding energies lower than 13.132 kcal mol 1 fail to form active ZFNs in human *** contrast,for arrays with calculated protein-DNA binding energies higher than 5 kcal mol 1,as many as 40% lacked ZFN activity in human ***,we suggest that the FoldX force field can be useful in reducing the failure rate and increasing efficiency in the design of ZFNs.

摘要：The hybrid CMOS molecular （CMOL） circuit, which combines complementary metal-oxide- semiconductor （CMOS） components with nanoscale wires and switches, can exhibit significantly improved performance. In CMOL circuits, the nanodevices, which are called cells, should be placed appropriately and are connected by nanowires. The cells should be connected such that they follow the shortest path. This paper presents an efficient method of cell allocation in CMOL circuits with the hybrid CMOS/nanodevice structure; the method is based on a cultural algorithm with chaotic behavior. The optimal model of cell allocation is derived, and the coding of an individual represent- ing a cell allocation is described. Then the cultural algorithm with chaotic behavior is designed to solve the optimal model. The cultural algorithm consists of a population space, a belief space, and a protocol that describes how knowledge is exchanged between the population and belief spaces. In this paper, the evolutionary processes of the population space employ a genetic algorithm in which three populations undergo parallel evolution. The evolutionary processes of the belief space use a chaotic ant colony algorithm. Extensive experiments on cell allocation in benchmark circuits showed that a low area usage can be obtained using the proposed method, and the computation time can be reduced greatly compared to that of a conventional genetic algorithm.

摘要：A novel radome is presented,which is transparent at operating frequency and is invisible out of *** order to prevent reflection of the incoming power,frequency selective surfaces loaded with the lumped resistors are *** obtain the pass-band properties in lower frequencies,the convoluted slots are *** comparison with the results obtained both by full wave analysis and by the measurements,the performance of the radome is *** performs with high transmission characteristics in band,and broadband absorbing properties out of band *** oblique incidences are also investigated for both transmission coefficients and reflection ones.

摘要：There is a consensus that the prevention of wound infection should be achieved in the following ways:(1)closing the wound to protect it from extra infection;(2)an antibacterial agent that could kill endogenous ***,existing bulk two-dimensional antibacterial materials show inefficient adhesion to wounds with complex morphology and thus cause the prevention of wound *** the thickness of bulk two-dimensional materials to less than 100 nanometres endows them with great flexibility,which could allow them to adhere to wounds with complex morphology by only physical ***,a broad-spectrum and efficient antimicrobial peptide(AMP)was introduced to biocompatible methacrylated gelatine(GelMA)with multiple modification sites,which served as an inner antibacterial *** being combined with a biodegradable and good mechanical poly-l-lactide(PLLA)outer layer through plasma-treatment-assisted spin coating,we finally constructed bilayered antibacterial nanosheets with a thickness of approximately 80 *** bilayered nanosheets possess good adhesion to surfaces with complex topography and thus achieve better wound closure than other bulk two-dimensional ***,this AMP-grafted conjugation shows minimal cytotoxicity compared with Ag^+antibacterial agents,and the antibacterial rate of nanosheets is dependent on the graft rate of *** suggest that this bilayered antibacterial nanosheet might be an advanced anti-infection dressing for wound treatment in clinical settings.

摘要：The fan-beam optical sensor is made up of many semiconductor lasers and detectors fixed around the wall alternately at a cross section of pneumatically conveying pipe. When the sensor works, a scanning light source emits a 50° lamellar fan-beam through the gas-solid two phase flow, and the projection data resulting extinction effect of solid particles are detected at the same time. With the projection data, the flow rate mass can be calculated, and then the flow image can be reconstructed. In this paper, the design of the sensor including spatial arrangement of the structural parts, basic principle and measurement sensitivity distribution are introduced. The mathematical measurement model of solid mass flow rate is presented together with the testing results.

摘要：A series of novel arylpiperazine derivatives was synthesized. The in vitro cytotoxic activities of all synthesized compounds against three human prostate cancer cell lines（PC-3, LNCa P, and DU145） were evaluated by a CCK-8 assay. Compounds 8, 10, 13, 17 and 20 exhibited strong cytotoxic activities against the tested cancer cell lines（IC_（50）〈3 μmol/L）. In addition, these compounds exhibited weak cytotoxic effects on human epithelial prostate normal cells WPMY-1. The structure-activity relationship（SAR） of these arylpiperazine derivatives was also discussed based on the obtained experimental data.

摘要：Gas turbine power units,as an effective way to cope with the severe challenge of renewable energy accommodation in power grids,arouse the interest of power enterprises in the deep peak-load regulation *** common alternative load-control strategies including constant turbine inlet temperature(TIT)and constant turbine exhaust temperature(TET)regulations were taken into *** comparatively investigate the part-load performance under these strategies,both mathematical and physical models were set up successively to serve as a validation and complementary to each *** the mathematical model of compressor with inlet guide vane(IGV),combustor and turbine,stage-stacking method based on blade average geometric parameter,energy conservation and turbine stage model were adopted *** the physical model,design and off-design analysis were carried out based on GT PRO and THERMOFLEX *** simulation result of mathematical model validated the reliability of the physical *** on this,the influence of ambient temperature and different load-regulating strategies on the off-design performance of gas turbine power units was studied in *** results in the case of a PG 9351FA gas turbine show that the ambient temperature has a great impact on system performance,i.e.,every 5℃ increase in the ambient temperature produces a reduction of 3.7%in the relative full-load output and 1.1%in the relative efficiency respectively;when the gas turbine operates under constant TIT strategy,TET starts to rise till it reaches the maximum allowable value with the load rate decreasing,and IGV keeps at the minimum angle with both TIT and TET decreasing when the load rate is lower than 65%;when the gas turbine operates under constant TET strategy,TIT drops slightly at load rate of above 60%while both TIT and TET evidently decrease below 60%load rate operating along the constant corrected speed line at the minimum allowable IGV opening;gas

摘要：A novel optimization-based method for designing wavelet filter banks in image fusion is proposed. The filter bank design is formulated as a nonlinear optimization problem. The objective function of the optimization problem consists of both the performance metrics of the image fusion, such as the root mean square error （RMSE）, and those of individual filters. The optimization problem is solved using simulating annealing.

摘要：Increasing attention has been paid to the air pollution more recently. Smog chamber has been proved as a necessary and effective tool to study atmospheric processes, including photochemical smog and haze formation. A novel smog chamber was designed to study the atmospheric photochemical reaction mechanism of typical volatile organic compounds(VOCs) as well as the aging of aerosols. The smog chamber system includes an enclosure equipped with black lights as the light source, two parallel reactors(2 m^3 of each) with separate control of light source and temperature, with a series of coupled instruments for online monitoring of gas phase and particle phase reactants and products. Chamber characterization, including air source stability, effective light intensity, temperature stability, as well as gas phase and particle phase wall losses, were carried out before further research. The results showed that our smog chamber systems developed by other domestic and international groups. It was also observed that the wall loss of aromatic VOCs varied with different functional groups as well as the isomerism. The results of preliminary simulation experiment from styrene-NO_x demonstrated that the chamber can be well utilized to simulate gas-particle conversion progresses in the atmosphere.

摘要：The quasi-zero-stiffness(QZS)vibration isolation has been proven to be an effective way to isolate low-frequency ***,most of the existing QZS isolators are space-consuming,which could not be employed in the space-limited *** this paper,a quite compact QZS isolator is engineered by connecting a pair of mutually repulsive magnet rings and a space-saving wave spring in parallel,called WQZS *** restoring force of the magnet ring is derived by the equivalent magnetic charge method,and an empirical formula for the restoring force of the wave spring is derived by the least squares method based on experimental *** dynamic model of the WQZS vibration isolation system is built,and the vibration isolation performance is evaluated through ***,an experimental prototype is fabricated,and the experimental tests on frequency sweep are carried *** results show that compared with the linear counterpart,the vibration isolation frequency of the WQZS isolator is reduced by 48.89%,and the peak transmissibility is decreased by 59.33%.Most importantly,the space occupancy of the WQZS isolator designed in this paper is much lower than that of the traditional QZS isolators,and thus it should be a potential solution for low-frequency vibration isolation in the space-limited environment.