摘要：目的了解神经源性膀胱患者使用自我管理微信小程序的感知好处,并识别阻碍其使用的关键因素。方法采用质性研究设计,通过目的抽样选取深圳市2所三级甲等医院康复科住院并试用自我管理微信小程序2周的19例神经源性膀胱患者进行半结构化访谈,运用内容分析法进行资料分析。结果患者认为该自我管理程序是有帮助的,同时存在使用的障碍因素。3个感知益处包括:方便、灵活、直观;促进患者自我管理;为照护者和家庭成员提供指导。阻碍患者使用该程序的障碍因素包括:患者消极的膀胱自我管理态度和患者特征;对移动医疗风险的担忧;小程序升级的必要性。结论神经源性膀胱患者使用微信小程序进行自我管理是可行的,可以满足患者在住院期间和出院后获取信息的需求。研究发现的自我管理程序使用促进和障碍因素,为医疗机构实施移动医疗干预措施促进神经源性膀胱患者的自我管理提供了有价值的信息。

摘要：AIM： To construct and produce a recombinant bispecific humanized single-chain Fv （sFv） /Interleukin-2 （IL-2） fusion protein by using mammalian cells. METHODS： The sFv/IL-2 protein was genetically engineered, and transfected to mammalian cells to determine whether the mammalian protein folding machinery can produce and secrete active sFv/IL-2 with high efficiency. RESULTS： The fusion protein was constructed and high efficiently expressed with yields up to 102 ±4.2 mg/L in culture supernatant of the stably transfected 293 cell line. This recombinant fusion protein consisted of humanized variable heavy （VH） and light （VL） domains of monoclonal antibody （mAb） 520C9 directed against the human HER-2/neu （c-erbB2） proto-oncogene product p185, and human IL-2 connected by polypeptide linker. The fusion protein was shown to retain the immunostimulatory activities of IL-2 as measured by IL- 2-dependent cell proliferation and cytotoxicity assays. In addition to its IL-2 activities, this fusion protein also possessed antigen-binding specificity against p185, as determined by indirect ELISA using p185 positive SKOV 3ip1 cells. CONCLUSION： The large-scale preparation of the recombinant humanized sFv antibody/IL-2 fusion protein is performed with 293 cells. The recombinant humanized sFv antibody/IL-2 fusion protein may provide an effective *** targeting therapeutic doses of IL-2 to p185 positive tumors without increasing systemic toxicity or immunogenicity.

摘要：The rapid recombination of photo-generated electron-hole pairs,insufficient active sites,and strong photocorrosion have considerably restricted the practical application of Cd S in photocatalytic ***,we designed and constructed a 2D/2D/2D layered heterojunction photocatalyst with cascaded 2D coupling *** using electron spin resonance spectroscopy,ultraviolet photoelectron spectroscopy,and in-situ irradiation X-ray photoelectron spectroscopy were conducted to confirm the 2D layered CdS/WO_(3) step-scheme(S-scheme)heterojunctions and CdS/MX ohmic ***,it was found that the strong interfacial electric fields in the S-scheme heterojunction photocatalysts could effectively promote spatially directional charge separation and transport between CdS and WO_(3) *** addition,2D Ti_(3)C_(2) MXene nanosheets with a smaller work function and excellent metal conductivity when used as a co-catalyst could build ohmic junctions with Cd S nanosheets,thus providing a greater number of electron transfer pathways and hydrogen evolution *** showed that the highest visible-light hydrogen evolution rate of the optimized MX-Cd S/WO_(3) layered multi-heterostructures could reach as high as 27.5 mmol/g/h,which was 11.0 times higher than that of pure CdS ***,the apparent quantum efficiency reached 12.0% at 450 *** is hoped that this study offers a reliable approach for developing multifunctional photocatalysts by integrating S-scheme and ohmic-junction built-in electric fields and rationally designing a 2D/2D interface for efficient light-to-hydrogen fuel production.

摘要：Hydrogel-Ⅰ was fabricated via sodium alginate in situ-embedding with MAL powders and then applied to decontaminating Pb(Ⅱ) from water. Conditions for preparing Hydrogel-Ⅰ and the adsorption of Pb(Ⅱ) over Hydrogel-I were optimized through response surface methodology coupled with central composite design. XPS revealed that the groups of —OH, —COO—, —NH, —NHand —CSS— carried by Hydrogel-Ⅰ were responsible for the uptake of Pb(Ⅱ). Ions exchange, surface complexation, electrostatic attraction and pore-filling effect contributed to the adsorption process. Adsorption performances of Pb(Ⅱ) by Hydrogel-Ⅰ and MAL powders were compared. Although they exhibited similar adsorption rate and maximum adsorption capacity(qm), the reusing ability of Hydrogel-Ⅰ was better and it was easier to be separated from aqueous solution after treatment. Even compared with organic hydrogel materials,Hydrogel-Ⅰ presented relatively quick adsorption speed and high adsorption capacity. It can be concluded that Hydrogel-Ⅰ could be an alternative scavenger for the treatment of Pb(Ⅱ) from aqueous solution.

摘要：Alloyed nanoparticles with core-shell structures provide a favorable model to modulate interfacial interaction and surface structures at the atomic level,which is important for designing electrocatalysts with high activity and ***,core-shell structured Pd3M@Pt/C nanoparticles with binary PdM alloy cores(M=Fe,Ni,and Co)and a monolayer Pt shell were successfully synthesized with diverse *** these,Pd3Fe@Pt/C exhibited the best oxygen reduction reaction catalytic performance,roughly 5.4 times more than that of the commercial Pt/C catalyst used as *** significantly enhanced activity is attributed to the combined effects of strain engineering,interfacial electron transfer,and improved Pt *** functional theory simulations and extended X-ray absorption fine structure analysis revealed that engineering the alloy core with moderate lattice mismatch and alloy composition(Pd3Fe)optimizes the surface oxygen adsorption energy,thereby rendering excellent electrocatalytic *** researches may use this study as a guide on the construction of highly effective core-shell electrocatalysts for various energy conversions and other applications.

摘要：Photocatalytic solar fuel generation is currently a hot topic because of its potential for solving the energy crisis owing to its low cost and zero-carbon ***,the rapid bulk recombination of photoexcited carrier pairs is a fundamental *** resolve this problem,we synthesized a dual cocatalysts system of cobalt phosphide(Co P)and molybdenum carbide(Mo_(2)C)embedded on strontium titanate(Sr TiO_(3))*** with those of pristine SrTiO_(3) and binary samples,the dual cocatalysts system(denoted SCM)showed a significant improvement in the hydrogen evolution and CO_(2) reduction ***,the structure of SCM effectively promoted spatial charge separation and enhanced the photocatalytic *** addition,the Schottky junction formed between the SrTiO_(3) and cocatalysts enabled the rapid transfer of photoexcited electrons from SrTiO_(3) to the cocatalysts,resulting in effective separation and prolonged photoexcited electron *** electron migration route between SrTiO_(3) and the cocatalysts was determined by in situ irradiation X-ray spectroscopy,and band structures of Sr TiO_(3) and the cocatalysts are proposed based on results obtained from UV-vis diffraction reflection spectroscopy and ultraviolet photoelectron spectroscopy *** the basis of our results,the dual cocatalysts unambiguously boosts charge separation and enhances photocatalytic *** summary,we have investigated the flux of photoexcited electrons in a dual cocatalysts system and provided a theoretical basis and ideas for subsequent research.

摘要：万尼尔构型旨在描述万尼尔函数在实空间上的局域位置及其携带的电荷信息,这一物理概念的提出为刻画天然及人工晶体结构中的波函数提供了全新的视角.在二维高阶拓扑绝缘体中,万尼尔构型中的分数化电荷可以作为一种内禀的拓扑不变量来表征湮灭在体连续谱中的角模式.本文报告了一项基于测量谱电荷分布观测声学体系中万尼尔构型的实验研究.作者在构建的声子晶体中测得了表现为分数化谱电荷的拓扑角模式反常,这种模式反常可以作为一种易于观测的实空间拓扑指标对湮灭在体态中的拓扑角模式进行先验判别.在此基础上,通过将不同的万尼尔构型按照多种方式进行组合,将原本湮没在体连续谱中的角模式调制至带隙中.在组合后的声子晶体中,平庸相和非平庸相结构均可以作为包覆层,从而为在带隙中构造和调控拓扑角模式提供了一种新思路.该研究有望应用于设计高品质因子声谐振腔、声通信以及传感增强等领域.

摘要：植物的生态过程依赖于叶片和根系的功能运行,但少有研究同时关注这两类性状在群落中的分布格局(CTD)。本文测定了广东亚热带森林44种共生木本植物的16个叶片和根系性状,旨在检验类比的叶片和根系性状是否耦合,是否沿类似的功能性状谱变异,是否表现出类似的CTD,其CTD和群落加权平均性状值(CWM)是否沿环境梯度表现出类似的响应。研究结果表明,叶片和根系性状变异的第一轴均呈显出由资源获取向资源保守变异的经济学谱,但叶片变异的第二轴主要由叶片碳含量表征,而根系变异的第二轴主要由反映菌根协作程度的根系粗细表征。类比的叶片和根系化学性状整体正相关,但形态性状的耦合程度较低。在群落水平上,叶片和根系性状第一轴的CWM均随海拔升高而变得更保守,而第二轴的变化则不一致。随土壤磷含量的降低或钾含量的升高,根系变得更细和在养分性状上更保守,而叶片性状无显著变化。当整合多维性状轴时,叶片性状呈发散格局,且随海拔、土壤磷和钾含量的增加呈收敛趋势;而根系性状呈收敛格局,但沿上述环境梯度呈发散趋势。该研究强调,叶片和根系性状与环境互作的差异可能导致不同的性状分布格局;植物可通过多样的地上和地下功能设计应对环境异质性。

摘要：Single-atom catalysts(SACs)have been widely used in heterogeneous catalysis owing to the maximum utilization of metal-active sites with controlled structures and well-defined *** tailored coordination with nitrogen atom,the metal-nitrogen(M-N)-based SACs have demonstrated interesting physical,optical and electronic properties and have become intense in photocatalysis and electrocatalysis in the past *** substantial efforts in constructing various M–N-based SACs,the principles for modulating the intrinsic photocatalytic and electrocatalytic performance of their active sites and catalytic mechanism have not been sufficiently ***,the present review intends to shed some light on recent research made in studying the correlation between intrinsic electronic structure,catalytic mechanism,single-metal atom(SMA)confinement and their photocatalytic and electrocatalytic activities(conversion,selectivity,stability and etc).Based on the analysis of fundamentals of M–N-based SACs,theoretical calculations and experimental investigations,including synthetic methods and characterization techniques,are both included to provide an integral understanding of the underlying mechanisms behind improved coordination structure and observed ***,the challenges and perspectives for constructing highly active M–N based photocatalysis and electrocatalysis SACs are *** particular,extensive technical and mechanism aspects are thoroughly discussed,summarized and analyzed for promoting further advancement of M-N-based SACs in photocatalysis and electrocatalysis.

摘要：[Introduction] Accurate calculation of the hydrodynamic coefficients for floating structures and the investigation of the flow field distribution around floating bodies on the marine free surface are essential for improving the engineering design and application of marine structures.[Method] This study utilized the computational fluid dynamics(CFD) approach and the Reynolds Averaged NavierStokes(RANS) method and considered the effects of viscosity and free surface interactions on the hydrodynamic behavior of floating *** employing the dynamic mesh technique,this study simulated the periodic movements of simplified three-dimensional(3D)shapes:spheres,cylinders,and cubes,which were representative of complex marine *** volume of fluid(VOF) method was leveraged to accurately track the nonlinear behavior of the free *** this analysis,the added mass and damping coefficients for the fundamental modes of motion(surge,heave,and roll) were calculated across a spectrum of frequencies,facilitating the fast determination of hydrodynamic forces and moments exerted on floating structures.[Result] The results of this study are not only consistent with the results of the 3D potential flow theory but also further reflect the role of *** method can be used for precise calculation of the hydrodynamic coefficients of floating structures and for describing the flow field of such structures in motion on a free surface.[Conclusion] The methodology presented goes beyond the traditional potential flow approach.