摘要：This review describes work presented in the 2014 inaugural Tsinghua university Press-Springer Nano Research Award lecture, as well as current and future opportunities for nanoscience research at the interface with brain science. First, we briefly summarize some of the considerations and the research journey that has led to our focus on bottom-up nanoscale science and technology. Second, we recapitulate the motivation for and our seminal contributions to nanowire- based nanoscience and technology, including the rational design and synthesis of increasingly complex nanowire structures, and the corresponding broad range of ＂applications＂ enabled by the capability to control structure, com- position and size from the atomic level upwards. Third, we describe in more detail nanowire-based electronic devices as revolutionary tools for brain science, including （i） motivation for nanoelectronics in brain science, （ii） demonstration of nanowire nanoelectronic arrays for high-spatial/high-temporal resolution extracellular recording, （iii） the development of fundamentally-new intracellular nanoelectronic devices that approach the sizes of single ion channels, （iv） the introduction and demonstration of a new paradigm for innervating cell networks with addressable nanoelectronic arrays in three-dimensions. Last, we conclude with a brief discussion of the exciting and potentially transformative advances expected to come from work at the nanoelectronics-brain interface.

摘要：The distant downstream proportional integral(PI) feedback control was applied to the W-M lateral canal of the Maricopa Stanfield Irrigation and Drainage District located in central Arizona,*** configuration of those controls for the ca-nal can be *** this end,an integrated approach to the design of an automatic control system for the canal was *** approach presented herein is not only a systematical review of previous work,but also represents a further ad-vance of the previous simulation study by Tsinghua university(Shang et al,2011) on relating the canal control algorithm to local PI controls for the real *** evaluate the control system potential,performance of the control algorithm that was ob-tained through implanting predicative module into linear quadratic regulator(LQR) was analyzed with singular value *** "manufactured" tests were conducted to compare with the control system that is currently in *** results indi-cated that the developed control system rather than the system in current use had considerable potential to closely match dis-charge at the downstream check structures with those ordered by water users while maintaining the water level throughout the length of the canal.

摘要：Copy number variants （CNVs） are pervasive in the human genome and are responsible for many Mendelian diseases and genomic disorders. The detection of CNVs is an essential element of a complete mutation screening strategy. Many techniques have been developed for gene dosage testing. Multiplex ligation-dependent probe amplification （MLPA） is a robust, easy and flexible technique that can detect both deletions and duplications for more than 40 loci in one assay. It has been widely used in research and diagnostic laboratories. We routinely develop our own MLPA assays for quick validation of array comparative genomic hybridization （CGH） findings. Here we discuss the general principles and critical aspects of MLPA assay development and validation using all synthetic MLPA probes. We believe that MLPA will play important roles in the rapid detection of genomic disorders associated with genomic imbalances, the confirmation of pathogenic mutations involving exonic deletions/duplications, CNV genotyping and population frequency analysis of CNVs.

摘要：Amidst the rapidly expanding integration of large language models(LLMs)across various sectors(ranging from everyday applications to specialized fields demanding stringent regulatory adherence),our investigation seeks to determine how well these models can support medical device software *** device classification functions to systematically categorize devices according to their designated use,associated risk levels,and requisite regulatory oversight,thereby providing a structured framework for ensuring safety and efficacy as mandated by regulatory authorities.

摘要：Ma Xiangbo was born in 1840 and became a pioneer of educational reform during the republican *** was responsible for introducing the idea that science and humanities should be valued equally in liberal arts education,a concept that became key to the model of university ***’s view of education combined Western humanism and science with classical Confucian *** ideas still have a referential value for contemporary Chinese higher education and society.

摘要：The human gut microbiota is widely considered to be a metabolic organ hidden within our bodies,playing a crucial role in the host’s *** factors affect its composition,so a wide variety of microbes residing in the gut are present in the world *** excessive imbalances in microbial composition are often associated with human disorders and pathologies,and new investigative strategies to gain insight into these pathologies and define pharmaceutical therapies for their treatment are *** vitro models of the human gut microbiota are commonly used to study microbial fermentation patterns,community composition,and host-microbe *** and microfluidic devices have been designed to culture microorganisms from the human gut microbiota in a dynamic environment in the presence or absence of eukaryotic cells to interact *** this review,we will describe the overall elements required to create a functioning,reproducible,and accurate in vitro culture of the human gut *** addition,we will analyze some of the devices currently used to study fermentation processes and relationships between the human gut microbiota and host eukaryotic cells.

摘要：In the spatial design fields such as architectural design and urban design, a consensus-building process among a variety of stakeholders like project executors, architects, residents, users, and general citizens is required. New technological developments such as cloud computing and Virtual Design Studios （VDS） enable the creation of virtual meeting systems. This paper proposes an approach towards a synchronous distributed design meeting system. In this paper, in addition to sharing a 3D virtual space for a synchronous distributed type design meeting, we developed a prototype system that enables participants to sketch or make annotations and have discussions as well as add viewpoints to them. We applied these functions to evaluate an architectural design and urban landscape examination. In conclusion, the proposed method was evaluated as being effective and feasible. Yet, it shows a few shortcomings including the fact that simultaneous operation is limited to one client, and more arbitrary shapes should be supported in future versions of the application.

摘要：The immune response after implantation of a biomaterial may shorten the functional life of the implant,depending on the degree of the *** this study,we used a polyacrylamide-alginate(PAAm-Alg)hydrogel,which has been previously characterized as a biocompatible material and shown to enhance regeneration of cartilage in vivo,along with a graphiteenhanced hydrogel(PAAm-Alg-G)as a non-biocompatible counterpart,to evaluate macrophage attachment and polarization to pro-or anti-inflammatory *** performance of each biomaterial in the presence of fibroblasts and chondrocytes was validated by an in vitro model which demonstrated modulation of the foreign-body response.A blend of 5%gelatin methacryloyl and 0.1%methacrylated hyaluronic acid was optimized to mimic the extracellular matrix(ECM)and support cell viability,proliferation,migration,and functionality at an initial concentration of 3.25×10^(5) cells/*** PAAm-Alg-G hydrogel localized in the simulated ECM showed cytotoxic and genotoxic effects for both fibroblasts and chondrocytes,while exhibiting a proliferative effect on macrophages with elevated immune *** M1/M2 ratio was 0.73 for PAAm-Alg hydrogel but 2.64 for PAAm-Alg-G,leading to significant M1 dominance(p0.05).The interleukin-6(IL-6)concentration secreted in the presence of PAAm-Alg hydrogel(4.58 pg/mL)significantly decreased(p<0.0001)on day 13,while the increase(p<0.0001)in interleukin-10(IL-10)concentration(120.73 pg/mL)confirmed the switch from a pro-inflammatory to an anti-inflammatory *** immune responses by developing a simplistic yet powerful three-dimensional in vitro model provides advantages in preparing for clinical use of biomaterials.

摘要：We report the development of a metastasis-on-a-chip platform to model and track hepatocellular carcinoma(HCC)-bone metastasis and to analyze the inhibitory effect of an herb-based compound,thymoquinone(TQ),in hindering the migration of liver cancer cells into the bone *** bioreactor consisted of two chambers,one accommodating encapsulated HepG2 cells and one bone-mimetic niche containing hydroxyapatite(HAp).Above these chambers,amicroporous membrane was placed to resemble the vascular barrier,where medium was circulated over the *** was observed that the liver cancer cells proliferated inside the tumor microtissue and disseminated from the HCC chamber to the circulatory flow and eventually entered the bone *** number of metastatic HepG2 cells to the bone compartment was remarkably higher in the presence of HAp in the *** was then used as ametastasis-controlling agent in both free form and encapsulated nanoparticles,to analyze its suppressing effect on HCC *** indicated that the nanoparticle-encapsulated TQ provided a longer period of inhibitory *** summary,HCC-bone metastasis-on-a-chip platform was demonstrated to model certain key aspects of the cancer metastasis process,hence corroborating the potential of enabling investigations on metastasis-associated biology as well as improved anti-metastatic drug screening.

摘要：mRNA疫苗因其免疫原性强、无基因整合风险和低成本制造等天然优势,已在传染病的预防以及癌症治疗等领域展示出巨大的潜力.目前,针对mRNA疫苗面临的稳定性差和蛋白表达效率不高等挑战,研究人员开展了广泛的mRNA设计和优化工作.传统的mRNA疫苗设计依赖于经验和实验结果的反复迭代,而随着人工智能技术的发展, mRNA疫苗设计正向更加理性和高效的方向转变.本文首先系统介绍了各种疫苗的特性,并概述了多款已上市和临床评估阶段的mRNA疫苗.同时,针对mRNA疫苗的优化问题,根据mRNA的五个不同结构区域,分别详细介绍了经验设计和人工智能设计在mRNA疫苗理性设计中的应用和优缺点.最后,讨论了人工智能模型在mRNA疫苗优化中的局限性和未来发展方向.