摘要：High enzymatic activity is required for laccase *** co.posite design （CCD）-based response surface methodology （RSM） can effectively increase the enzymatic activity of Pleurotus ostreatus P40 in liquid substrate *** screening of the nutritional co.ponents was performed using a Plackett-Burman *** variables,namely,bran,bagasse,Tween 80,and yeast extract,were found to have statistically significant effects on laccase *** variables were further optimized using CCD-based *** co.centrations for the maximum laccase activity were 8.144 2 g/L bran,50 g/L bagasse,0.424 1 mL/L Tween 80,and 2.832 5 g/L yeast *** optimized co.ditions,the maximum measured laccase activity reached 96 480 U/L,which was close to the predicted value （104 830 U/L） by ***,RSM can be used to optimize culture co.ponents for laccase activity from Pieurotus ostreatus P40.