摘要：Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(***),***:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic *** by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), *** strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three *** hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of *** in Iran.

摘要：AIM： To investigate the correlation between ASCA and presence of mucosal S. cerevisiae DNA in a population of CD, ulcerative colitis （UC） patients and controls. METHODS： S. cerevisiae-specific primers and a fluorescent probe were designed for a 5＇ exonuclease real time PCR （TaqManTM） assay, which is a homogenous system using a fluorescent-labelled probe for the detection of PCR product in real time. We analyzed the relation of the PCR results with the ASCA findings in a group of 76 inflammatory bowel disease （IBD） patients （31 CD, 45 UC） and 22 healthy controls （HC）. RESULTS： ASCA （IgA or IgG） were positive in 19 （61%） patients with CD, 12 （27%） with UC and none of the HC. PCR amplification was inhibited and excluded from the final results in 10 （22%） UC patients, 7 （22%） CD patients, and 6 （30%） HC. In only 15 of the mucosal samples, S. cerevisiae DNA was detected by real time PCR, including 7 （29%） in CD, 7 （19%） in UC, 1 （6%） in HC. In 4 CD and in 4 UC patients, ASCA and mucosal S. cerevisiae were positive. Hucosal S. cerevisiae was present in combination with negative ASCA IgA and IgG in 3 UC, and 3 CD patients. CONCLUSION： We conclude that since the presence of S. cerevisiae in colonic mucosal biopsy specimens is very rare, ASCA is unlikely to be explained by continuous exposure to S. cerevisiae in the mucosa. Therefore, ASCA formation must occur earlier in life and levels remain relatively stable thereafter in immunological susceptible persons.

摘要：设计并合成一系列新型八氢吡咯[3,2-c]吡啶衍生物作为趋化因子受体(CCR5)抑制剂,并测试其抗人免疫缺陷病毒(HIV-1)生物活性.大多数化合物显示了抗HIV-1活性,其中,八氢吡咯[3,2-c]吡啶衍生物19c活性最好(IC50<1μmol·L-1).但在研究其药物作用机理时发现,该化合物除作用于CCR5外,还可作用于其他抗HIV-1靶标.另外,利用计算机模拟、分子对接方法,对该系列化合物进行了初步的构效关系讨论.

摘要：【目的】设计乳酸杆菌属特异性T-RFLP技术(末端限制性片段长度多态性分析)对14株乳酸杆菌进行分型。【方法】采用源于16S-23S rRNA基因间隔区序列的乳酸杆菌属特异性引物LAB-rev,乳酸杆菌的属特异性引物,6-FAM荧光标记后结合16S上游通用引物7f用于乳酸杆菌的PCR扩增。【结果】选取HaeⅢ和HhaⅠ进行限制性酶切,最后对酶切后的产物末端测序得到T-RFLP峰谱图,该图谱能够快速准确地对不同种的乳酸杆菌进行定性、定量的分析。【结论】实验成功搭建T-RFLP技术用于微生态环境中乳酸杆菌检测的平台,对于在功能性食品、乳酸饮料和药物对肠道微生态的影响及菌种鉴定等领域有重大意义。

摘要：目的:检查左旋氧氟沙星在体外的抗分支杆菌活性。设计:用7H11培养基,以琼脂稀释方法测左旋氧氟沙星对不同种的分支杆菌的最小抑制浓度(MIC),并与氧氟沙星比较。左旋氧氟沙星对于被小鼠腹腔巨噬细胞吞食的结核分支杆菌和胞内分支杆菌的抗微生物活性是以菌落形成单位(CFU)的减少来衡量的。结果:左旋氧氟沙星抗结核分支杆菌、堪萨斯分支杆菌、海鱼分支杆菌、瘰疬分支杆菌、鸟分支杆菌、胞内分支杆菌、偶然分支杆菌和龟分支杆菌的MIC比氧氟沙星的MIC低2到4倍。左旋氧氟沙星减少巨噬细胞内CFU的效果高于氧氟沙星。结论:左旋氧氟沙星比氧氟沙星具有更强的体外抗分支杆菌活性。

摘要：流感病毒和新型冠状病毒(SARS-CoV-2)是近年来频繁引发呼吸道疾病的主要病原,其引起的黏膜免疫应答对于病毒的控制和清除至关重要.目前对于SARS-CoV-2感染后肺部黏膜免疫应答的具体调控机制及其与流感感染的区别尚不清楚我们通过原位杂交-多重免疫荧光染色技术及空间转录组学揭示了流感病毒和SARS-CoV-2感染过程中局部免疫应答的动态变化过程,并发现相比于流感病毒感染,SARS-CoV-2感染早期肺部黏膜会更加快速地形成诱导性支气管相关淋巴组织(iBALT)结构并活化B1细胞产生较高水平IgM和IgA,可能在抗病毒感染及iBALT的形成中发挥重要作用.同时,SARS-CoV-2及表达其S蛋白的假病毒感染均能够促进小鼠和人的B1细胞的活化.将表达S蛋白的腺病毒疫苗AdC7-S对小鼠进行免疫后,可以成功诱导出包含B1细胞的iBALT,并在病毒感染中发挥保护作用.本研究揭示了呼吸道病毒引起的肺脏黏膜免疫动态应答过程,并阐明了SARS-CoV-2感染早期B1细胞的活化特点及免疫保护作用,为揭示新发呼吸道传染病的保护性应答机制及疫苗设计提供了理论基础.

摘要：Malaria still threatens global health seriously *** the current discoveries of antimalarials are almost totally focused on single mode-of-action inhibitors,multi-targeting inhibitors are highly desired to overcome the increasingly serious drug ***,we performed a structure-based drug design on mitochondrial respiratory chain of Plasmodium falciparum and identified an extremely potent molecule,RYL-581,which binds to multiple protein binding sites of *** simultaneously(allosteric site of type Ⅱ NADH dehydrogenase,Q_(o) and Q_(i) sites of cytochrome bc_(1)).Antimalarials with such multiple targeting mechanism of action have never been reported ***-581 kills various drug-resistant strains in vitro and shows good solubility as well as in vivo *** structurebased strategy for designing RYL-581 from starting compound may be helpful for other medicinal chemistry projects in the future,especially for drug discovery on membrane-associated targets.

摘要：A plant fermentation was carried out by Yeast and Lactobaccilli against fermented black turmeric, Kaempferia parviflora (FBT). These materials were proved by as safe in animal safety experiment. We tried to investigate changes of immune-competent cells that commonly utilized FBT, including after administration of immno-suppressed animals, the effects by FBT on the regulated effect on the cells were evaluated. Our results showed that FBT augmented the level of lymphocytes in number, while FBT regulated the level of granulocytes in both number and function. In our clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio suggesting their constitution as neutral in peripheral blood were increased significantly 30 days after the administration of FBT in rodents, and compromised host was prepared with cancer chemotherapeutic agent (Mytomycin-C). Our observations showed against intracellular parasite, and that FBT augmented intercellular pathogen through humoral immunity. We discussed the significance and mechanism of FBT on the level of leukocyte subsets in number and function that were considered to be potential indicators for the activation of the compromised host. We also proposed an idea that FBT exhibited tonic effects via activating complement components. The evidences were shown by immune-electrophoretic method. Moreover, we tried to access further to the anti-oxidative activities of this FBT. This modification brought to the significant lift up for antibody producing cells and anti-oxidative activity for phagocytic cells.

摘要：抑动蛋白是小瓜虫(Ichthyophthiriusmultifiliis)体表纤毛的主要构成部分,也是宿主免疫系统发挥抗虫免疫时的主要识别抗原。根据已经报道的3种抑动蛋白N端及C端保守的多肽片断,设计了一对简并引物P6/P7,以小瓜虫分离株IchFJ9的基因组为模板,成功扩增了抑动蛋白编码区(ORF)的全长基因序列。扩增的iagFJ9基因全长1398bp,编码466个氨基酸,包含18个非标准密码子TAA(Glu,Q)推导的氨基酸序列包含6个以CPXGT为起始的串联重复单位,具有抑动蛋白的特征性结构。同源比较分析显示,扩增的iagFJ9基因与已知的小瓜虫抑动蛋白基因IAG52A基因具有88%同源率。证实了iagFJ9是一个新发现的抑动蛋白基因,用简并PCR技术去发现小瓜虫抑动蛋白基因家族新基因是可行的。

摘要：目的克隆肺炎衣原体基因CPn0308,表达融合蛋白,并制备抗体对其表达的内源性蛋白进行初步定位。方法根据STD基因库提供的信息设计引物,聚合酶链反应(PCR)克隆目的基因,用BamHI/NotI对克隆的目的基因和pGEX-6P2载体进行酶切,T4连接酶连接后,重组质粒经42℃转化XL1-blue细菌;用PCR进行初步筛选,交叉PCR对提取质粒进一步确定,最后对插入片断进行序列分析;用IPTG诱导阳性克隆的细菌使其表达GST融合蛋白,纯化后免疫小鼠制备抗体,使用IFA对内源性蛋白进行定位分析。结果克隆出肺炎衣原体基因CPn0308,全长为366bp,编码121个氨基酸;并表达了融合蛋白GST-CPn0308,分子量约为39kD;制备了抗体,IFA实验发现该蛋白初步定位于肺炎衣原体包涵体膜蛋白上。结论成功克隆肺炎衣原体基因CPn0308,其内源性蛋白初步定位于肺炎衣原体包涵体膜上。