摘要：We analyzed the advertisement call of Paa spinosa at Yuliang Mountain,Lanxi,Zhejiang Province,in eastern *** and spectral call parameters were analyzed,along with call *** comprised of three to seven notes,the last of which had the longest *** formants(harmonics)were clearly distinguishable from the audio *** dominant frequency ranged from 411-1534 Hz,and was either the first or the second *** number of notes within a call was positively correlated to air temperature,so that calls contained more notes during the ***,the dominant frequency appeared to be lower at the highest temperature.

摘要：The eukaryotic genome is packaged into a complex nucleoprotein structure named chromatin, balancing the compactness of genome and the accessibility of regulatory proteins and RNA polymerases to DNA. The mechanisms of the regulation of chromatin dynamics include the post-translational modification of histones, alteration of nucleosome positions by chromatin remodelers, replacement of canonical histones by histone variants with the aid of histone chaperones, and dynamic organization of the three-dimensional genome in the small nucleus. Histone variants are different from canonical histones by substitution of several amino acid residues or changes in amino acid sequence. Histone variants perform specialized functions such as altering nucleosome stability, dynamics, structure, as well as playing critical roles in a range of biological processes like transcriptional regulation, DNA repair and recombination, development and immune responses. Here we discuss how histone variants, their modification and specific loading to chromatin are involved in transcriptional regulation, DNA repair and plant development.

摘要：The clustered regularly interspaced short palin- dromic repeats （CRiSPR）-associated system enables biologists to edit genomes precisely and provides a powerful tool for perturbing endogenous gene regulation, modulation of epigenetic markers, and genome architecture. However, there are concerns about the specificity of the system, especially the usages of knocking out a gene. Previous designing tools either were mostly built-in websites or ran as command-line programs, and none of them ran locally and acquired a user- friendly interface. In addition, with the development of CRISPR-derived systems, such as chromosome imaging, there were still no tools helping users to generate specific end-user spacers. We herein present researchers to design primers CRISPR Primer Designer for for CRISPR applications. Theprogram has a user-frfendly interface, can analyze the BLAST results by using multiple parameters, score for each candidate spacer, and generate the primers when using a certain plasmid. In addition, CRISPR Primer Designer runs locally and can be used to search spacer clusters, and exports primers for the CRiSPR-Cas system-based chromosome imaging system.

摘要：在 Tarim Riverare 的中间、更低的活动范围的河走廊区域在 Ruoqiang 从 Qara 水库 inYuli 县沿着河节由传统的绿走廊统治了到泰泰马·莱克县，它在 Tarim 河分水岭在社会、经济的发展起一个重要作用与一极其重要的生态的功能。对水路垂直的缓冲区地区从水路在 5-kminterval 开始沿着两个河边被定义(10 km 更宽) 用缓冲区地区分析的方法。每个缓冲区地区的风景模式索引被计算，并且主要风景模式的索引被分析以便在风景和补丁层次上揭示放射的效果和河走廊的特征。放射的宽度通常是在学习区域的 30 km 的结果表演，和罐头直到在潜水艇的 50-70 km -- 区域 FG1 因为学习区域位于二排水盆，与在他们之间的一个生态的空隙的地区。树林和沼泽地的 Thelandscapes 通常在 10 km 缓冲区地区以内被散布(它意味着缓冲区地区是离开水路的 5 km；一样此后) 沿着两个河边。而且，缓冲区地区越靠近到水路，补丁密度和最大的补丁索引是越多 higher。在为学习区域的生态、环境的新生，河走廊的生态的效果的程度必须充分被考虑，并且工程必须科学地并且讲道理地被计划并且设计。

摘要：Aquaculture,the most rapidly growing food production sector,has exhibited tremendous expansion over the past several decades and it currently accounts for more than half of the global fish production for human *** ensure sustainable aquaculture,it is necessary to breed farmed fish species(strains)with valuable economic traits,such as high production,high quality,disease resistance,and stress *** fish genetic breeding techniques largely rely on key processes including understanding the genetic basis of economically important traits to the generation and selection of favorable genotypes.

摘要：Designing regional pattern is challenging for ecosystem restoration both in theory and in practice. The present study proposed a new approach to design regional pattern for ecosystem restoration with a case in the arid valley of Upper Minjiang River, SW China. The procedures are: (1) Determine the regional pattern of main environmental factors based on the relationships between the important environmental factors and the main geographical factors generated from DEM data. (2) Overlay the maps of the main environmental factors. (3) Generate a lookup table according to the species selection rule we proposed to link the candidate restoration species to the main environmental factors.(4) Determine the regional pattern of ecosystem restoration by positioning the restoration species in the map of environmental factors according to the lookup table. The regional restoration pattern designed in this way would be applicable not only to restoring a region, but also to predicting the future landscape change.

摘要：Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the in vivo concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into in vivo biosensors of small molecules, which couple the intracel- lular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screen- ing of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.

摘要：On the basis of many years of research at the laboratory, and by making reference to the related literature published at home and abroad, in this paper the concept of rice ideal panicle type was introduced, and the principles of rice ideal panicle type design were elucidated. The parame-ters of rice ideal panicle type for Liaoning were preliminarily established as follows: Panicle length 2 mm, large vascular bundle and primary branch >15 pieces/panicle, primary branch grain >80 grains/panicle, secondary branch grains concentrating in the middle and upper parts of panicle axis, and panicle type index >0.5. In addition, the possibility of achieving unity of grain yield and quality at a still higher level by creating an ideal panicle type was explored.

摘要：Maize(Zea mays L.) stands prominently as one of the major cereal crops in China as well as in the rest of the ***,predicting the growth and yield of maize for large areas through yield components under high-yielding environments will help in understanding the process of yield formation and yield potential under different environmental *** accurate early assessment of yield requires accuracy in the formation process of yield components as *** order to formulate the quantitative design for high yields of maize in China,yield performance parameters of quantitative design for high grain yields were evaluated in this study,by utilizing the yield performance equation with normalization of planting *** density was evaluated by parameters including the maximum leaf area index and the maximum leaf area per *** showed that the variation of the maximum leaf area per plant with varying plant density conformed to the Reciprocal Model,which proved to have excellent prediction with root mean square error(RMSE) value of 5.95%.Yield model estimation depicted that the best optimal maximum leaf area per plant was 0.63 times the potential maximum leaf area per plant of *** performance parameters for different yield levels were quantitatively designed based on the yield performance *** validation of the yield performance model by simulating high yields of spring maize in the Inner Mongolia Autonomous Region and Jilin Province,China,and summer maize in Shandong Province,the yield performance equation showed excellent prediction with the satisfactory mean RMSE value(7.72%) of all the *** present study provides theoretical support for the formulation of quantitative design for sustainable high yield of maize in China,through consideration of planting density normalization in the yield prediction process,providing there is no water and nutrient limitation.

摘要：The cis-acting regulatory elements, e.g., promoters and ribosome binding sites （RBSs） with various desired properties, are building blocks widely used in synthetic biology for fine tuning gene expression. In the last decade, acquisition of a controllable regulatory element from a random library has been established and applied to control the protein expression and metabolic flux in different chassis cells. However, more rational strategies are still urgently needed to improve the efficiency and reduce the laborious screening and multifaceted characterizations. Building precise computational models that can predict the activity of regulatory elements and quantitatively design elements with desired strength have been demonstrated tremendous potentiality. Here, recent progress on construction of cis- acting regulatory element library and the quantitative predicting models for design of such elements are reviewed and discussed in detail.