摘要：The paper presents implementation of CD technology in injection moulding of micro-pattern polymer substrates for application in microfluidic device used for cell immobilization, manipulation and subsequent investigation. The paper aims to develop a design concept of the micro-pattern polymer substrates with specially located thin-sections. Microstructure for positioning of cells is located in the thin sections and is consistent with the possibility the substrate to be produced by injection molding. For this purpose an appropriate microstructure and the thickness of the substrate thin-sections are chosen according the impact of geometrical parameters and the location of microstructure. They are optimized by study their dependence on the parameters of the gate system and the substrate thickness on the process of mould filling. By the CD-based models developed a series of values of microstructure parameters and thin-section parameters were obtained. Relevant versions of the substrate design with cell-grooves are achieved. Results obtained states that the reduction the cell-grooves size and increasing the number of the thin-sections makes the mould filling difficult or impossible. In this case, it is advisable, the polymer substrate and thin-sections to be molded by injection molding, then cell-grooves can be further shaped by hot embossing.