摘要：研究和鉴定分枝杆菌与其宿主间相互作用的生物分子,对于确定分枝杆菌在入侵过程中的作用至关重要,并为相关疾病生物学和发病机制提供基础知识。分枝杆菌是一种古老的病原体,它为自己在宿主和环境中生存发展了复杂的策略,这可能与其参与感染分子之间相互作用网络的复杂性有关。在结核病检测技术的发展过程中,一些生物标记物已经开始受到关注。研究最广泛的抗原有CFP-10、ESAT-6、Ag85A、Ag85B、CFP-7和PPE18。其中一些抗原已被作为生物标记来评估病原体和宿主感染反应。设计新颖而准确的诊断方法对控制全世界的结核病至关重要。目前,诊断方法是基于对感染个体体液反应分子的识别。因此,这些测试依赖于宿主产生免疫反应的能力,而免疫反应通常是异质的。在过去的20年里,人们特别重视多抗原诊断方法的设计,以提高敏感性和特异性水平。本文综述了结核分枝杆菌生物分子的研究和应用现状,以期为结核控制提供新的支持。

摘要：The objective of this paper relies on the establishment of the sampling moment and the nutritional state of the species Conilon coffee (Coffea canephora) cultivated in Cambisoils. The effect of different rates of nitrogen fertilization was also studied in the leaves concentrations of N, at randomized complete block designs (N 0 = 0; N 1 = 50; N 2 = 100; N 3 = 150 and N 4 = 200 kg?ha -1 of N) in presence of P (40 kg?ha -1 of P 2 O 5 ) and K (160 kg?ha -1 of K 2 O), with four replicates. The samples were carried out in the months of March (flower blossoming stage), June (fruits growing stage), September (grain development) and december (maturation) in all treatments. Foliar analysis carried out in the month of June (fruits growing stage) for the purpose of establishing critical nutrient range values for N, settling down approaches of interpretation of the same one for the Conilon coffee plantations on Cambisoils. The biggest percentages in the yields are obtained with leaves containing N between 2.80% and 3.10%, a value below 2.80% N which they associated with indicative percentages of stable maximum yield that did not surpass the limits of the adequate nutrients supply.