摘要：Based on a theoretical motion equation of encapsulated microbubbles within an ultrasound field, the subharmonic characterizations of microbubbles are optimally designed and analyzed by a computer aided design system. The effects of size, shell elasticity and acoustic pressure on subharmonic response of microbubbles are calculated theoretically to obtain the optimal parameters for nondestructive subharmonic imaging. In addition, microbubbles with different shell elasticity are prepared, and their subharmonic responses are measured in vitro. The results of theoretical calculation and acoustic measurement show that good subharmonic enhancement can be obtained by using the encapsulated microbubbles with the mean size of 3 μm, which were prepared from the surfactant solution with the proper ratio of shell material. It is also shown that the best operating acoustic pressure is 200 to 400 kPa for nondestructive subharmonic imaging based on such kind of microbubbles.

摘要：Dermal substitutes have provided a template for the regeneration and reconstruction of the ***,the healed skin tissue often exhibits abnormal morphology and functionality,including scarring and *** this study,a composite bioink composed of methacrylated gelatin(GelMA)and chitosan oligosaccharide(COS)was proposed for printing a dermal scaffold using digital light processing(DLP)*** GelMA/COS bioink exhibited suitable porosity,swelling,degradation rate,and mechanical *** inclusion of COS demonstrated antibacterial effects against both Gram positive and Gram-negative bacteria,while simultaneously fostering the proliferation of human dermal fibroblasts(HDFs).Additionally,the application of COS could effectively reduce the expression levels of fibrosis-related genes,such as collagen I,collagen III,and fibronectin *** three-dimensionally printed cell-laden dermal scaffold exhibited excellent shape fidelity and high cellular viability,facilitating the extension of HDFs along the scaffold and the simultaneous secretion of extracellular matrix ***,the HDF-laden dermal scaffold transplanted into full-thickness skin defect sites in nude mice was shown to accelerate wound closure,reduce inflammation,and improve wound ***,the DLP-printed dermal scaffold provides an appealing approach for effectively treating full-thickness skin defects in clinical settings.

摘要：Molybdenum-based catalysts have demonstrated significant potential in the electrocatalytic hydrogen evolution reaction(HER).However,the limited exposure of active sites and strong hydrogen adsorption result in suboptimal ***,a Mo_(2)N±MoSe_(2) heterojunction is prepared on carbon cloth(MNS/CC)to enhance the *** strong electronic interaction between Mo_(2)N and MoSe_(2),combined with the lower work function of Mo_(2)N,creates an intrinsic electric field at the heterojunction interface,which markedly improves charge transfer ***,the optimized electronic structure of Mo sites further enhances charge transfer and intrinsically catalytic activity in *** a result,MNS/CC requires overpotentials of mere 65 and 210 mV to achieve current densities of 20 mA cm^(−2) and 1 A cm^(−2),respectively,with a Tafel slope of only 96 mV dec−***,MNS/CC maintains stable operation at 1 A cm^(−2) for 240 h without significant *** results offer insights into the design of non-precious metal-based electrocatalysts for industrial hydrogen production.

摘要：For the surgical treatment of cardiovascular disease(CVD),there is a clear and unmet need in developing small-diameter(diameter<6 mm)vascular *** our previous work,sulfated silk fibroin(SF)was successfully fabricated as a potential candidate for preparing vascular grafts due to the great cytocompatibility and ***,vascular graft with single layer is difficult to adapt to the complex internal *** this work,polycaprolactone(PCL)and sulfated SF were used to fabricate bilayer vascular graft(BLVG)to mimic the structure of natural blood *** enhance the biological activity of BLVG,nicorandil(NIC),an FDA-approved drug with multi-bioactivity,was loaded in the BLVG to fabricate NIC-loaded *** morphology,chemical composition and mechanical properties of NIC-loaded BLVG were *** results showed that the bilayer structure of NIC-loaded BLVG endowed the graft with a biphasic drug release *** in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation,migration and antioxidation capability of endothelial cells(ECs).Moreover,we found that the potential biological mechanism was the activation of PI3K/AKT/eNOS signaling ***,the results effectively demonstrated that NIC-loaded BLVG had a promising in vitro performance as a functional small-diameter vascular graft.

摘要：biomedical metals are widely used as implant materials in the human or animal body to repair organs and restore function, such as heart valves, meninges, peritoneum and artificial *** element affects the microstructure, mechanical property, corrosion resistance and wear resistance, but also influences the antibacterial and biological ***, antibacterial metal alloys have shown great potential as a new kind of biomedical materials, in which Cu has been widely used as antibacterial agent *** addition, biodegradable metal alloys, including magnesium alloy and zinc alloy, also have attracted much attention *** was also used as alloying element to adjust the degradation ***, the role of Cu in the alloy design will be very important for the development of new *** this paper, we summarized the recent research results on the Cu-containing metal alloy for biomedical application and hoped that this review would give more suggestions for the further development of biomedical metal alloy.

摘要：Theranostic nanosystems that integrate diagnosis and therapy have garnered increasing attention for personalized *** integration of the versatile nanoparticles to fabricate self-assembled theranostic nanomedicines becomes increasingly important in current medical *** silica nanoparticles(MSN)with their highly attractive physicochemical properties and favorable morphological attributes represent ideal templates for the controlled assembly and integration of functional nanomaterials to fabricate self-assembled theranostic *** rationally designed combination strategy and heterostructure will improve the overall bioavailability and preserve the unique property of each *** this review,the cutting-edge strategies for the designed fabrication of MSN-templated self-assembled nanomedicines are *** categorize MSN-based nanomedicines by their unique heterostructures,including core-shell,yolk-shell,core-satellite,heterodimer and core-shell-satellite structures,and discuss the controlled assembly approaches as well as the intriguing applications for disease ***,a perspective on the challenges in the clinical translation of self-assembled theranostic nanomedicines is highlighted.

摘要：Cortical electrodes are a powerful tool for the stimulation and/or recording of electrical activity in the nervous ***,the inevitable wound caused by surgical implantation of electrodes presents bacterial infection and inflammatory reaction risks associated with foreign body ***,inflammation of the wound area can dramatically worsen in response to bacterial *** consequences can not only lead to the failure of cortical electrode implantation but also threaten the lives of ***,we prepared a hydrogel made of bacterial cellulose(BC),a flexible substrate for cortical electrodes,and further loaded antibiotic tetracycline(TC)and the anti-inflammatory drug dexamethasone(DEX)onto *** encapsulated drugs can be released from the BC hydrogel and effectively inhibit the growth of Gram-negative and Gram-positive ***,therapeutic cortical electrodes were developed by integrating the drug-loaded BC hydrogel and nine-channel serpentine arrays;these were used to record electrocorticography(ECoG)signals in a rat *** to the controlled release of TC and DEX from the BC hydrogel substrate,therapeutic cortical electrodes can alleviate or prevent symptoms associated with the bacterial infection and inflammation of brain *** approach facilitates the development of drug delivery electrodes for resolving complications caused by implantable electrodes.

摘要：水凝胶作为承载组织的替代产品引起了广泛关注.然而,与能够通过有限的变形来承受复杂载荷的承载组织不同,传统的水凝胶由于其柔软的特性,无法在低应变状态下表现出刚性响应.基于承载组织的软硬组分之间的协同作用,我们设计了采用软基质(正泊松比)和硬框架(负泊松比)的复合水凝胶,其刚度分别比基质和框架高18.9和4.2倍.相反,结合软基质(正泊松比)和框架(正泊松比)的设计对刚度增强有限.通过有限元分析,揭示了软基质与框架之间正、负泊松比斥力作用的内在机理.本研究为今后设计基于软硬组分协同效应的复合水凝胶提供了思路.

摘要：Current electro spun membranes used for pulp capping still lack the sustained-release capability and long-term anti-inflammatory effects that are favorable for dental pulp *** this work,a single-layered poly(lac tic acid)(PLA)electro spun membrane loaded with amorphous calcium phosphate(ACP)and aspirin(PLA/ACP/Aspirin membrane,i.e.,PA A membrane)is sandwiched between two poly(lactic-co-glycolic acid)(PLGA)electro spun membranes as a novel sandwich-structured PLGA and PA A composite electro spun membrane(PLGA-PAA membrane)to resolve the need for sustained-release design and anti-inflammatory *** angle measurements indicate that the PLGA-PAA membrane is more hydrophilic than the PAA *** in vitro release study reveals that PLGA membranes coated on PAA membrane could slightly slow down ion release,while signiificantly prolonging aspirin *** also co-cultured membranes with dental pulp stem cells(DPSCs)and human monocytic THP-1 cells to evaluate their osteogenic ability and anti-inflammatory effects,*** with the PAA membrane,the PLGA-PAA membrane promotes cell adhesion,proliferation,and osteogenic differentiation.A prolonged anti-inflammatory effect of up to 18 days is also observed in the PLGA-PAA *** results suggest a promising strategy for fabricating an electro spun membrane system with controlled release capabilities and long-term anti-inflammatory effects for use as pulp-capping material for regeneration of the dentin-pulp complex.

摘要：Rapid formation of a continuous endothelial cell(EC)monolayer with healthy endothelium function on the luminal surface of vascular implants is imperative to improve the longtime patency of small-diameter vascular *** the present study,we combined the contact guidance effects of aligned nanofibers,which enhance EC adhesion and proliferation because of its similar fiber scale with native vascular basement membranes,and aligned microfibers,which could induce EC elongation effectively and allow ECs *** was followed by successive immobilization of collagen IV and laminin to fabricate a biomimetic basement membrane(BBM)with structural and compositional *** hemolysis assay and platelet adhesion results showed that the BBM exhibited excellent ***,the adhered human umbilical vein endothelial cells(HUVECs)onto theBBMaligned along the orientation of the microfibers with an elongated morphology,and the data demonstrated that the BBM showed favorable effects on EC attachment,proliferation,and *** oriented EC monolayer formed on the BBM exhibited improved antithrombotic capability as indicated by higher production of nitric oxide and prostacyclin(PGI2).Furthermore,fluorescence images indicated that HUVECs could infiltrate into the BBM,implying theBBM’s ability to enhance transmural ***,theBBMpossessed the properties to regulate ECbehaviors and allow transmural ingrowth,demonstrating the potential to be applied as the luminal surface of small-diameter vascular implants for rapid endothelialization.