摘要：Tillage practices can potentially afect soil organic carbon (SOC) accumulation in agricultural soils. A 4-year experiment was conducted to identify the influence of tillage practices on SOC sequestration in a double-cropped rice (Oryza sativa L.) field in Hunan Province of china. Three tillage treatments, no-till (NT), conventional plow tillage(PT), and rotary tillage(RT), were laid in a randomized complete block design. Concentrations of SOC and bulk density(BD) of the 0-80 cm soil layer were measured, and SOC stocks of the 0-20 and 0-80 cm soil layers were calculated on an equivalent soil mass(ESM) basis and fixed depth (FD) *** carbon budget(SCB) under diferent tillage systems were assessed on the basis of emissions of methane(CH4) and CO2 and the amount of carbon (C) removed by the rice harvest. After four years of experiment, the NT treatment sequestrated more SOC than the other treatments. The SOC stocks in the 0-80 cm layer under NT (on an ESM basis) was as high as 129.32 Mg C ha 1,significantly higher than those under PT and RT (P PT > RT,and the same order was observed for SCB; however, in the 0-20 cm soil layer, the RT treatment had a higher SOC stock than the PT treatment. Therefore, when comparing SOC stocks, only considering the top 20 cm of soil would lead to an incomplete evaluation for the tillage-induced efects on SOC stocks and SOC sequestrated in the subsoil layers should also be taken into consideration. The estimation of SOC stocks using the ESM instead of FD method would better reflect the actual changes in SOC stocks in the paddy filed, as the FD method amplified the tillage efects on SOC stocks. This study also indicated that NT plus straw retention on the soil surface was a viable option to increase SOC stocks in paddy soils.