摘要：In this paper,a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported.A tandem genes cluster CHS-CHI-IFS(rIFS)of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase(CHS),chalcone isomerase(CHI),and isoflavone synthase(IFS)(GenBank accession numbers EU526827,EU526829,EU526830)in only one recombination with the pET22b *** resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions,and the genistein-like com-ponent was detected.

摘要：Parkinson’s disease(PD)is the second largest degenerative disease of central nervous system around the world.α-Synuclein(α-syn)is the main component of Lewy body amyloid deposits in the brain of PD patients and associated with the pathogenesis of PD.α-Syn aggregates can induce neuron death and cause irreversible neurological ***,the inhibition ofα-syn aggregation and removal of preformed amyloid fibers are important strategies for the prevention and treatment of ***,there is still a lack of potent inhibitors to interfere withα-syn *** this work,we demonstrate that lentinan(LNT),a natural polysaccharide extracted from the fruit body of Lentinula edodes,can potently preventα-syn aggregation,disassemble preformed amyloid aggregates,and suppressα-syn-mediated cell toxicity and reactive oxygen species ***,we also find that the molecular weight of the polysaccharide impacts the inhibitory effect of *** in vivo investigation demonstrates that LNT significantly inhibits the accumulation ofα-syn in nematodes ***,relieves the symptoms of PD nematode motility disorders,and effectively protects dopaminergic neurons from *** PD *** the best of our knowledge,this is the first time to report that LNT can modulateα-syn aggregation in vitro and in vivo,which will provide new insights for designing and developing potential agents for PD treatment.