摘要：The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective *** a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns,HIV virus like particles(VLPs) offer an attractive alternative because they are considered safer since they lack viral *** got a stable eukaryotic cell line by G418 resistance selection,engineered to express the HIV-1 structure protein Gag and Env efficiently and *** confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into *** VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant.