摘要：Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis *** the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection is mainly dependent on molecular approaches such as reverse transcription polymerase chain reaction(RT-PCR)and reverse transcription quantitative real-time polymerase chain reaction(RTqPCR).The widely used primers and probes for RT-qPCR were established in the early *** HuNoVs are highly variant viruses,viral genome mutations result in previously designed primers and/or probes that were perfectly matched working less efficiently over *** this study,a new duplex RT-qPCR(ND-RT-qPCR)was designed for the detection of genogroup Ⅰ(GⅠ)and genogroup Ⅱ(GⅡ)HuNoVs based on an analysis of viral sequences added in the database after *** long transcribed viral RNAs,the results demonstrate that the sensitivity of ND-RT-qPCR is as low as one genomic copy for both GⅠ and GⅡ *** performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer/probe sets for RT-qPCR(Kageyama RT-qPCR)for 23 HuNoV-positive clinical *** five GⅠ samples were registered as positive by ND-RT-qPCR,whereas only two samples were registered as positive by Kageyama *** 18 GⅡ samples were registered as positive by ND-RT-qPCR,while 17 samples were registered as positive by Kageyama *** sensitivity reflected by the quantification cycle(Cq)value was lower in ND-RT-qPCR than in Kageyama *** data suggest that ND-RT-qPCR could be a good fit for the detection of current strains of HuNoVs.

摘要：Photosynthetic terpene production represents one of the most carbon and energy-efficient routes for converting CO_(2)into *** photosynthetic organisms,metabolic engineering has led to limited success in enhancing terpene productivity,partially due to the low carbon *** this study,we employed systems biology analysis to reveal the strong competition for carbon substrates between primary metabolism(e.g.,sucrose,glycogen,and protein synthesis)and terpene biosynthesis in Synechococcus elongatus PCC *** then engineered key“source”and“sink”***“source”limitation was overcome by knocking out either sucrose or glycogen biosynthesis to significantly enhance limonene production via altered carbon ***,a fusion enzyme complex with geranyl diphosphate synthase(GPPS)and limonene synthase(LS)was designed to further improve pathway kinetics and substrate *** synergy between“source”and“sink”achieved a limonene titer of 21.0 mg/***,the study demonstrates that balancing carbon flux between primary and secondary metabolism can be an effective approach to enhance terpene bioproduction in *** design of“source”and“sink”synergy has significant potential in improving natural product yield in photosynthetic species.

摘要：One of the most important issues regarding the design of engineered landfill is landfill leachate that is produced when water passes through the waste. Landfill Leachate comprises a mass of different organic and inorganic components which can be dissolved or suspended. Regardless of the nature of the components, it poses a potential pollution on local land and surface waters. The present work was undertaken to assess, isolate, identify and characterize the hydrocarbon degrading bacteria associated with environmental samples collected from discharge of Ain Temouchent, Algeria. The samples （landfill leachate） were chemically and microbiologically analyzed using standard techniques. These organisms were further studied to determine the level of biodegrading activities on hydrocarbons as the single carbon source using enrichment medium under different physical and chemical parameters （pH, temperature, NaCI and tolerance of crude oil...）, The identification of seven strains isolated named as Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudornonas sp., Staphylococcus aureus, Staphylococcus xylosus, Staphylococcus hominis and Staphylococcus warnerii. These strains growth on a neutral medium and temperature between 30 ℃ and 37 ℃, 5 at 6% of NaCI concentration, and can use a concentration of 5 at 10% of crude oil as sole source of carbon.

摘要：微生物电合成(microbial electrosynthesis,MES)利用可再生电力驱动微生物固定CO_(2)合成化学品,在推进碳循环经济中具有一定潜力,受到广泛关注。但是,很少有研究通过高效反应器设计来促进产乙酸并降低能耗。本研究中,新型流动电极MES反应器的总产乙酸速率[(16±1)g·m^(-2)·d^(-1)]比无粉末活性炭(powder activated carbon,PAC)对照[(8±3)g·m^(-2)·d^(-1)]高两倍。流动电极MES反应器的库伦效率为43.5%±3.1%,能量消耗为(0.020±0.005)k Wh·g^(-1),产乙酸的能量效率为18.7%±1.3%。基于PAC的流动电极能够降低水跨膜通量、传质阻力,但是对装置电压、流变行为、乙酸吸附的影响较小。流动电极MES反应器中,能量代谢相关基因高表达,Acetobacterium的丰度增加。MES反应器中同时存在用于碳固定的还原性乙酰辅酶A途径(Wood–Ljungdahl pathway,WLP)与还原性三羧酸循环途径(reductive citric acid cycle,r TCA)。堆叠型流动电极MES中的乙酸浓度达7.0 g·L^(-1)。本研究提供一种构建可扩展MES反应器的新方法,促进CO_(2)利用以及产物生成。