摘要：Since 3D printed hard materials could match the shape of bone,cell survival and fate determination towards osteoblasts in such materials have become a popular research *** this study,a scaffold of hardmaterial for 3D fabrication was designed to regulate developmental signal(Notch)transduction guiding osteoblast *** established a polycaprolactone(PCL)and cell-integrated 3D printing system(PCI3D)to reciprocally print the beams of PCL and cell-laden hydrogel for a *** PCI3D module holds good cell viability of over 87%,whereas cells show about sixfold proliferation in a 7-day *** osteocytic MLO-Y4 was engineered to overexpress Notch ligand Dll4,making up 25%after mixing with 75%stromal cells in the PCI3D *** Dll4,unlike other delta-like family members such as Dll1 or Dll3,promotes osteoblast differentiation and themineralization of primary mouse and a cell line of bone marrow stromal cells when cultured in a PCI3D module for up to 28 ***,osteocytic Dll4 could not promote osteogenic differentiation of the primary bone marrow stromal cells(BMSCs)after conditional deletion of the Notch transcription factor RBPjκby Cre *** data indicate that osteocytic Dll4 activates RBPjκ-dependent canonical Notch signaling in BMSCs for their oriented differentiation towards ***,osteocytic Dll4 holds a great potential for angiogenesis in human umbilical vein endothelial cells within *** study reveals that osteocytic Dll4 could be the osteogenic niche determining cell fate towards *** will open a new avenue to overcome the current limitation of poor cell viability and low bioactivity of traditional orthopedic implants.

摘要：As an emerging technology to promote the combination of medicine and industry,the three-dimensional(3D)printing has developed rapidly in the fields of orthopedics,while its unique advantages in improving precise treatment still need to be further *** this report,our team have exhibited several classic cases of integrating 3D printing into orthopedic clinical application,thereby further elaborating thoughts and opinions on the significance of 3D printing in the orthopedic clinical application,technical advantages,existing main problems and coping strategies.

摘要：Gradual distraction with an external fixator is a widely used treatment for severe postburn ankle contracture(SPAC).However,application of external fixators is complex,and conventional two-dimensional(2D)imaging-based surgical planning is not particularly helpful due to a lack of spatial *** purpose of this study was to evaluate the surgical planning process for this procedure with patient-specific three-dimension-printed models(3DPMs).In this study,patients coming from two centers were divided into two cohorts(3DPM group *** group)depending on whether a 3DPM was used for preoperative surgical *** duration,improvement in metatarsal-tibial angle(MTA),range of motion(ROM),the American Orthopedic Foot and Ankle Society(AOFAS)scores,complications,and patient-reported satisfaction were compared between two *** 3DPM group had significantly shorter operation duration than the control group((2.0±0.3)h vs.(3.2±0.3)h,P<0.01).MTA,ROM,and AOFAS scores between the two groups showed no significant differences pre-operation,after the removal of the external fixator,or at *** feet were achieved and gait was substantially improved in all patients at the final ***-tract infections occurred in two patients(one in each group)during distraction and were treated with wound care and oral *** in the 3DPM group reported higher satisfaction than those in the control group,owing to better patient-surgeon *** planning using patient-specific 3DPMs significantly reduced operation duration and increased patient satisfaction,while providing similar improvements in ankle movement and function compared to traditional surgical planning for the correction of SPAC with external fixators.

摘要：背景和目的:非酒性脂肪肝病(NAFLD)是最常见的慢性肝病,同时也是代谢综合征的一项危险因素。NAFLD在全球范围内的患病率基于地域差异在5%~30%不等。尽管流行病学资料显示代谢相关性疾病的患病率在新加坡呈快速增长,但对于该地区NAFLD的数据却十分有限。本研究旨在了解新加坡NAFLD的患病率及公众对该病的认识。方法:本研究是一项横断面观察研究,旨在评估NAFLD患者的人口统计学、人体测量学及临床资料,研究对象来自于一项胃肠公众论坛的参会者。NAFLD的诊断基于超声学标准;代谢综合征的诊断则基于国际糖尿病联盟的指南。通过自行设计的调查问卷来了解大家对NAFLD这种疾病的看法。结果:共有227名研究对象纳入研究,其中40%被诊断为NAFLD。相对于那些非NAFLD人群,NAFLD者男性居多,年龄更大,体质指数更高,腰围更宽,患代谢性综合征的比例更高(均P0.05)。值得注意的是,75.6%的具有代谢危险因素者,并不认为自己存在NAFLD风险。结论:我们的研究显示NAFLD在新加坡具有较高的患病率,包括在那些非肥胖人群中。考虑到人们对NAFLD的发生风险认识不足,有必要加强公众教育,以提高人们对NAFLD的认识。

摘要：Overview and research infrastructure As one of the leading laboratories in the interdisciplinary field of additive manufacturing and bio-3D printing,the Bio-Manufacturing Center at Tsinghua University is highly dedicated to conducting cutting-edge research in the emerging field of *** latter is comprised of research involving biomaterials,living cells,proteins,and/or other biological compounds as basic building blocks to fabricate biomimetic structures,in vitro functional biological models and/or cellular systems with application to tissue engineering,regenerative medicine,disease pathogenesis,drug screening,and tissue/organ-on-a-chip.

摘要：This paper proposes an additive nanomanufacturing approach to fabricate a personalized lab-on-a-chip fluorescent peptide nanoparticles (f-PNPs) array for simultaneous multi-biomarker detection that can be used in Alzheimer’s disease (AD) diagnosis. We will discuss optimization techniques for the additive nanomanufacturing process in terms of reliability, yield and manufacturing efficiency. One contribution of this paper lies in utilization of additive nanomanufacturing techniques to fabricate a patient-specific customize-designed lab-on-a-chip device for personalized AD diagnosis, which remains a major challenge for biomedical engineering. Through the integrated bio-design and bio-manufacturing process, doctor’s check-up and computer-aided customized design are integrated into the lab-on-a-chip array for patient-specific AD diagnosis. In addition, f-PNPs with targeting moieties for personalized AD biomarkers will be self-assembled onto the customized lab-on-a-chip through the additive nanomanufacturing process, which has not been done before. Another contribution of this research is the personalized lab-on-a-chip f-PNPs array for AD diagnosis utilizing limited human blood. Blood-based AD assessment has been described as “the holy grail” of early AD detection. This research created the computer-aided design, fabrication through additive nanomanufacturing, and validation of the f-PNPs array for AD diagnosis. This is a highly interdisciplinary research contributing to nanotechnology, biomaterials, and biomedical engineering for neurodegenerative disease. The conceptual work is preliminary with intent to introduce novel techniques to the application. Large-scale manufacturing based on the proposed framework requires extensive validation and optimization.