摘要：Based on the building principle of additive manufacturing,printing orientation mainly determines the tribological properties of joint *** this study,we created a polyether-ether-ketone(PEEK)joint prosthesis using fused filament fabrication and investigated the effects of printing orientation on its tribological properties using a pin-on-plate tribometer in 25% newborn calf *** ultrahigh molecular weight polyethylene transfer film is formed on the surface of PEEK due to the mechanical capture of wear debris by the 3D-printed groove morphology,which is significantly impacted by the printing orientation of *** the printing orientation was parallel to the sliding direction of friction,the number and size of the transfer film increased due to higher steady *** transfer film protected the matrix and reduced the friction coefficient and wear rate of friction pairs by 39.13%and 74.33%,***,our findings provide a novel perspective regarding the role of printing orientation in designing knee prostheses,facilitating its practical applications.

摘要：合成生物学是借助基因工具改造活细胞和生物体的学科,其经历了蓬勃发展阶段,在生物医学领域得到广泛应用,包括新型生物传感器和生物材料设计,以及最新临床治疗药物和疫苗的开发.纳米材料具有独特的物理、化学和生物特性,在拓展合成生物学的生物医学应用场景上具有巨大的潜力,可以提高治疗剂的生产效率,并增强改造生物体的功能.在此,我们简要概述了纳米材料应用于合成生物学领域的最新进展,主要涵盖纳米材料作为递送载体、信号转导器和功能增强剂的角色及其制备过程和性能特点.本综述首先强调了纳米材料作为基因载体的最新成果,包括有机、无机和仿生递送系统;其次,回顾了纳米材料介导的刺激响应型基因表达调控的研究;第三,总结了在可编程的杂化生物活性材料方面的开创性工作,包括纳米材料/细菌杂合系统和纳米材料/哺乳动物细胞杂合系统;最后,讨论了该领域当前面临的挑战,并从个人观点展望了未来的发展前景.

摘要：Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor ***,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor *** study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I *** also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and ***,it compares two-dimensional(2D)and 3D *** comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D ***,the two models yielded significantly different cell ***,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D *** results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture *** developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

摘要：Touch-sensitive screens are crucial components of wearable *** such as reduced graphene oxide(rGO),carbon nanotubes(CNTs),and graphene offer promising solutions for flexible touch-sensitive ***,when stacked with flexible substrates to form multilayered capacitive touching sensors,these materials often suffer from substrate delamination in response to deformation;this is due to the materials having different Young’s modulus *** results in failure to offer accurate touch screen *** this work,we demonstrate an induced charge-based mutual capacitive touching sensor capable of high-precision touch *** is enabled by electron trapping and polarization effects related to mixed-coordinated bonding between copper nanoparticles and vertically grown graphene ***,we used an electron cyclotron resonance system to directly fabricate graphene-metal nanofilms(GMNFs)using carbon and copper,which are firmly adhered to flexible *** being subjected to 3000 bending actions,we observed almost no change in touch *** screen interaction system,which has a signal-to-noise ratio of 41.16 dB and resolution of 650 dpi,was tested using a handwritten Chinese character recognition trial and achieved an accuracy of 94.82%.Taken together,these results show the promise of touch-sensitive screens that use directly fabricated GMNFs for wearable devices.

摘要：将细胞封装在生物材料中用于器官修复或疾病治疗是一个新兴领域.目前,微流控技术被用于精确控制封装细胞的数量、大小和支架结构.微流控技术提供了设计生物仿生形态的能力,增强封装细胞的活性和功能,从而提高疾病治疗的效果.本文报道了微流控技术在仿生细胞封装方面的最新进展.我们首先简要概述了各种天然衍生的促进细胞功能的仿生结构,如受到细胞、植物、动物和自然反应启发的结构;之后讨论了基于微技术的最新细胞封装方法,并详细描述了相关过程;最后提出了仿生细胞封装在疾病治疗和临床应用中面临的挑战和未来方向.

摘要：目的调查相关营养评估指标与卒中后抑郁(poststroke depression,PSD)的关系,分析营养指标预测老年缺血性脑卒中患者发生PSD的表现,为护理人员早期识别PSD高危患者,优化脑卒中管理,改善预后提供参考依据。方法于2022年9月至2023年5月在深圳市1所医院神经内科选取239例老年缺血性脑卒中患者为研究对象。采用自行设计的问卷,收集患者一般人口学资料及疾病相关信息;采用白蛋白、微型营养评定简表(Mini Nutritional Assessment Short Form,MNA-SF)、老年营养风险指数(Geriatric Nutrition Risk Index,GNRI)评估患者的营养状况;采用医院焦虑抑郁量表的抑郁分量表(Hospital Anxiety and Depression Scale-Depression,HADS-D)评估卒中后抑郁情况。采用二元逻辑回归分析,分析卒中后抑郁相关影响因素,并采用受试者工作特征曲线分析方法比较营养指标的表现。结果老年缺血性脑卒中患者平均年龄为71.10±7.41岁,男性占66.5%(159/239),PSD发生率为32.6%(78/239)。不同营养指标的低值组PSD的发生率显著大于高值组,差异均具有统计学意义(P<0.05)。二元逻辑回归分析表明白蛋白(OR,0.681;95%CI,0.508~0.913,P=0.010)、GNRI(OR,1.238;95%CI,1.034~1.483,P=0.020)、MNA-SF(OR,0.708;95%CI,0.614~0.815,P<0.001)是影响PSD的因素(P<0.05)。白蛋白、GNRI和MNA-SF 3个指标联合预测老年缺血性脑卒中患者PSD的ROC曲线下面积最大,有较高的区分度(AUC,0.738;敏感度为75.6%,特异度为60.9%)。结论由白蛋白、GNRI、MNASF联合组成的营养评价指标可作为预测老年缺血性脑卒中营养不良患者发生PSD风险的辅助工具。需要护理人员在更多样化的患者群体中进行进一步的验证,以证明预测的准确性。

摘要：Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

摘要：A combination of hydrogels and microfluidics allows the construction of biomimetic three-dimensional(3D)tissue models in vitro,which are also known as *** hydrogel patterningwith awell-controlled spatial distribution is typically achieved by embedding sophisticated microstructures to act as a ***,these physical barriers inevitably expose cells/tissues to a less physiologically relevant microenvironment than in vivo ***,we present a novel dissolvable temporary barrier(DTB)strategy that allows robust and flexible hydrogel patterning with great freedom of design and desirable flow stimuli for cellular *** key aspect of this approach is the patterning of a water-soluble rigid barrier as a guiding path for the hydrogel using stencil printing technology,followed by a barrier-free medium perfusion after the dissolution of the *** and multiple tissue compartments with different geometries can be established using either straight or curved DTB *** effectiveness of this strategy is further validated by generating a 3D vascular network through vasculogenesis and angiogenesis using a vascularized microtumor *** a new proof-of-concept in vasculature-on-a-chip,DTB enables seamless contact between the hydrogel and the culture medium in closed microdevices,which is an improved protocol for the fabrication ofmultiorgan ***,we expect it to serve as a promising paradigm for organ-on-a-chip devices for the development of tumor vascularization and drug evaluation in the future preclinical studies.

摘要：Three-dimensional(3D)printing has attracted increasing research interest as an emerging manufacturing technology for devel-oping sophisticated and exquisite architecture through hierarchical *** has also been employed in various advanced industrial *** development of intelligent biomedical engineering has raised the requirements for 3D printing,such as flexible manufacturing processes and technologies,biocompatible constituents,and alternative ***,state-of-the-art 3D printing mainly involves inorganics or polymers and generally focuses on traditional industrial fields,thus severely limiting applications demanding biocompatibility and *** this regard,peptide architectonics,which are self-assembled by programmed amino acid sequences that can be flexibly functionalized,have shown promising potential as bioinspired inks for 3D ***,the combination of 3D printing and peptide self-assembly poten-tially opens up an alternative avenue of 3D bioprinting for diverse advanced ***,a small but innovative nation,has significantly contributed to 3D bioprinting in terms of scientific studies,marketization,and peptide architectonics,including modulations and applications,and ranks as a leading area in the 3D bioprinting *** review summarizes the recent progress in 3D bioprinting in Israel,focusing on scientific studies on printable components,soft devices,and tissue *** paper further delves into the manufacture of industrial products,such as artificial meats and bioinspired supramolecular architectures,and the mechanisms,physicochemical properties,and applications of peptide ***,Israel contributes significantly to the field of 3D bioprinting and should thus be appropriately recognized.

摘要：清蒲公英种子的良好飞行能力归功于其冠毛结构特征.以往研究主要集中于风从蒲公英种子的冠毛结构底部通过至顶部(简称为“一型风”)时,空气流动与种子的相互作用.然而,由于大气流和种子结构特征的多样性,蒲公英种子从花序头部脱落或空中飞行过程中,风从蒲公英冠毛结构顶部通过至底部(简称为“二型风”)的情况也很常见.但是,目前尚缺乏二型风情况下空气流动与种子相互作用的相关研究.本文中,我们通过数值模拟,考虑风速、冠毛结构角度和孔隙率的变化,研究和比较了在两种风型下冠毛结构模型的拖电力系数和周围流场分布特征。结果表明:由于风向的反转,流场分布特征和拖电力系数会发生显著变化.当风从第一型转为第二型时,冠毛丝对气流的干扰从背风侧移前至迎风侧,导致在第二型风时涡环大多数情况下附着在非扁平冠毛结构模型的中心圆盘上,而在第一型风时则发生涡环分离。当风从第一型变为第二型时,压力诱导的拖电力对总拖曳力的贡献显著增加,从而增强了非扁平冠毛结构模型的拖电力系数.然而,随着风速、冠毛结构角度和孔隙率的增加,这种增强作用会逐渐减弱.这些发现将有助于种子风驱扩散的定量预测,并为微型仿生飞行器的设计提供一定指导.