摘要：Rapid advances in DNA synthesis techniques have allowed the assembly and engineering of viral and microbial *** eukaryotic organisms,with their larger genomes,abundant transposons,and prevalent epigenetic regulation,present a new frontier to synthetic *** synthetic genomics have long been proposed,and exciting progress has been made using the top-down *** this perspective,we propose applying bottom-up genome synthesis in multicellular plants,starting from the model moss Physcomitrium patens,in which homologous recombination,DNA delivery,and regeneration are possible,although further optimizations are *** then discuss technical barriers,including genome assembly and plant transformation,associated with synthetic genomics in seed plants.