摘要：AIM： To study the effect of adenovirus （Ad）-p53 gene therapy on hepatocellular carcinoma （HCC） in a rabbit model.METHODS： VX2 tumor was grown in the liver of 24rabbits. Animals were divided into four groups： group A receiving trans-arterial gene therapy （Ad-p53） only,group B receiving combined Ad-p53 therapy and transarterial embolization （lipiodol）, group C receiving transarterial chemoembolization （lipiodol ＋ mitomycin C）,control group （D） receiving sodium chloride. Tumor volume （V1） was measured by using MRI （d 13）.Interventional procedure was applied （d 14）.Tumor volume （V2） was assessed by MRT （d 21） and the mean ratio （V2/V1） was calculated. After the second MRI,specimens of the liver were abstained and examined immunohistochemically using mutant-type p53 *** positive expression was scored.RESULTS： Compared with control group （（^-x） = 3.14± 0.64）, therapeutic groups all showed a significant decrease in the tumor growth ratio （P 〈 0.05）. A slight difference was found between group A （（^-x） =2.35 ±0.59） and group B （（^-x） = 1.75 ± 0.28） （P = 0.048）. Nostatistically significant difference was observed between group B and group C （（^-x） = 2.00 ± 0.44）. The positive expression rate of mutant-type p53 was the lowest in group B and significantly different between group A and group C （P 〈 0.05）.Compared to the control subjects, groups A and C both showed a decrease in the expression of mutant-type p53, but there was no significant difference between them.CONCLUSION： Trans-arterial Ad-p53 gene therapy can reduce tumor growth of HCC in rabbit model.

摘要：A series of hydrazine and oxadiazole analogs of Sorafenib was designed, synthesized and characterized by proton nuclear magnetic resonance（1H NMR） spectrometry and high resolution mass spectrometry（HRMS）. The anti-proliferative activities of these compounds against human colorectal earcinoma（HCT-116） and human breast cancer （MDA-MB-231） tumor cell lines were evaluated in vitro by MTT method[MTT=3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide]. The bioassay results suggest that most of the synthesized compounds have antitumor potential to HCT-116 cell line compared with MDA-MB-231 cell line. Compounds 8a, 8b, 8d, Be, 9f and 9j competitive with Sorafenib demonstrated antiproliferative activities on HCT-116 cell line.

摘要：Backgrou nd Dense titanium(Ti)fusion cages have been commonly used in transforaminal lumbar interbody ***,the stiffness mismatch between cages and adjacent bone endplates increases the risk of stress shielding and cage *** The current study presents a multiscale optimization approach for porous Ti fusion cage development,including microscale topology optimization based on homogenization theory that obtains a unit cell with prescribed mechanical properties,and macroscale topology optimization that determines the layout of framework structure over the porous cage while maintaining the desired *** biomechanical performance of the designed porous cage is assessed using numerical simulations of fusion *** laser melting is employed to assists with fabricating the designed porous structure and porous *** The simulations demonstrate that the designed porous cage increases the strain energy density of bone grafts and decreases the peak stress on bone *** mechanical and morphological discrepancies between the as-designed and fabricated porous structures are also *** From the perspective of biomechanics,it is demonstrated that the designed porous cage contributes to reducing the risk of stress shielding and cage *** optimization of processing parameters and post-treatments are required to fabricate the designed porous *** present multiscale optimization approach can be extended to the development of cages with other shapes or materials and further types of orthopedic implants.

摘要：Background We previously showed that nano-hydroxyapatite/carboxymethyl chitosan （n-Ha/CMCS） displayed excellent mechanical properties, good degradation rates and exceptional biocompatibility, with negligible toxicity. The aim of this study was to determine the effect of the same composite with vascular endothelial growth factor （VEGF）transfected bone marrow stromal cells （BMSCs） in a rabbit radial defect model.

摘要：To the Editor:Coronary computed tomographic angiography(CCTA)has been considered as one of the most important noninvasive imaging modalities in diagnosing coronary artery disease(CAD).[1]Modern scanner of CCTA can provide precise coronary atherosclerotic plaque information,showing improved diagnostic accuracy and sensitivity for identifying obstructive CAD with a preferable temporal and spatial *** studies have demonstrated the prognosis value of CCTA for the prediction of future adverse CAD events.

摘要：目的探讨反义寡核苷酸对胆囊癌细胞端粒酶活性的影响及对胆囊癌细胞生长增殖的作用。方法设计针对端粒酶RNA亚基模板序列并经硫代磷酸修饰的反义、正义和随机序列寡核苷酸,并以正常人成纤维细胞作对照,观察其对人胆囊癌细胞(GBCSD)端粒酶活性和细胞生长增殖的影响作用以及对正常人成纤维细胞的作用。结果反义寡核苷酸可有效地抑制胆囊癌细胞的端粒酶活性,呈剂量依赖性,并明显的抑制胆囊癌细胞的生长,对正常人成纤维细胞生长无明显作用。结论硫代反义寡核苷酸(PSODN)对胆囊癌端粒酶活性有明显的抑制用并促进细胞凋亡。