摘要：Bone is known to have a natural function to heal ***,if the bone damage is beyond a critical degree,intervention such as bone grafting may be *** this work,the fabrication of a novel bone scaffold composed of natural bone components and polycaprolactone(PCL)using 3D printing is put forward.α1,3-galactosyltransferase deficient pigs were used as the donor source of a *** porcine bone(DCB)with attenuated immunogenicity was used as the natural component of the scaffold with the aim to promote bone *** 3D printed DCB-PCL scaffolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive *** biological properties of the DCB-PCL scaffolds were evaluated by studying cell adhesion,viability,alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem *** in vitro results demonstrated that the DCB-PCL scaffolds exhibit an enhanced performance in promoting bone differentiation,which is correlated to the DCB ***,critical-sized cranial rat defects were used to assess the effect of DCB-PCL scaffolds on bone regeneration in *** results confirm that in comparison with PCL scaffolds,the DCB-PCL scaffolds can significantly improve new bone formation in cranial ***,the proposed 3D printed DCB-PCL scaffolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects.

摘要：Autograft or metal implants are routinely used in skeletal ***,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering *** use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-dimensional(3D)bioprintingof skeletal tissueis anattractivestrategyfor ***,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic *** this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based *** inclusion of the nanofiller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon *** composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)*** inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven *** study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

摘要：Green design and manufacturing is a proactive approach to minimize wastes during a product’s design stage, thus preventing future environmental impacts. Current modular design method mainly focuses on product functional and manufacturing issues. In this paper, a theoretical scheme of multi-objective modularity analysis for discrete electromechanical product design was proposed. Product physical architecture was represented by a fuzzy graph, where fuzzy relationships contain environmental objectives and influence module formulation. Finally the optimal product modules combining all objectives can be searched by clustering algorithm.

摘要：目的:探讨具有柔性的定制化钛下颌骨替代物的可行性,旨在兼顾支架整体强度的基础上,向患者自体骨提供有益于骨改建的应力刺激,促进患者自体骨与受骨的融合、改建。方法:结合骨改建理论提出一种通过改变钛下颌骨体部结构以增加骨替代物柔性的优化设计方案,制定一套以最佳应变体积比和最大许用应力为评价指标的评价体系。运用有限元优化分析法分析五种骨替代物结构,并对计算结果进行比较。结果:判断最易促进骨改建的柔性结构为直回形槽结构,并获得最佳直槽结构尺寸。结论:基于有限元方法的结构设计准则有效保证了骨替代物的植入安全性,并在此前提下最大限度的消减应力屏蔽现象,有利于促进植骨的改建。