摘要：Objective： To explore the role of transcription-coupled repair （TCR） pathway in the UVC-induced SupF gene mutation in Tet-on 293 cell line, we designed and constructed a Tet-responsive plasmid DNA pTCR-C1, and utilized this pTCR-C 1 plasmid to obtain the mutation frequency of SupF reporter gene induced by UVC in Tet-on 293 cell line. Methods： SupF gene was cloned into a Tet-responsive plamid pBI-L, which include a bidirectional Tet-responsive promoter, and was named pTCR-C1. The pTCR-C1 plasmid was transfected into Tet-on 293 cell line, and the mutation frequency of SupF reporter gene was detected in the presence and absence of DOX. Results： The pTCR-C1 plasmid was identified with the methods of restriction digestion and DNA sequencing. The mutation frequency of SupF reporter gene in the presence of DOX was higher than in the absence of DOX. Conclusion： The TCR pathway takes part in the UVC-induced SupF gene mutation in Tet-on 293 cell line.

摘要：Objective： To study biomechanical changes of newly formed bones 24 weeks after repairing large defects of long bones of goats using heterogeneous deproteinated bone （DPB） prepared by modified methods as an engineering scaffold. Methods： According to a fully randomized design, 18 goats were evenly divided into three groups： normal bone control group （Group A）, autologous bone group （Group B） and experimental group （Group C）. Each goat in Groups B and C were subjected to the periosteum and bone defect at middle-lower part of the right tibia （20% of the whole tibia in length）, followed by autologous bone or DPB plus autolognus MSCs ＋ rhBMP2 implantation, respectively and semi- ring slot fixation; while goats in Group A did not perform osteotomy. At 24 weeks after surgery, biomechanical tests were carried out on the tibias. Results： At 24 weeks after surgery, the results of anticompression test on tibias in three groups were recorded by a functional recorder presented as linear pressure-deformation curve. The shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values were 10.74 MPa±1.23 MPa, 10. 11 MPa±1.35 MPa and 10.22 MPa±1.32 MPa and fracture compression rates were 26.82%±0.87%, 27.17%±0.75% and 28.22%±1.12% in Groups A, B and C, respectively. Comparisons of anti-compression ultimate pressures and fracture compression rates among three groups demonstrated no significant difference （PAB=0.415, PBC=0.494）. Three-point antibend test on tibias was recorded as load-deformation curves, and the shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values of the anti-bend test were 481.52 N±12.45 N, 478.34 N±14.68 N and 475.62 N±13.41 N and the fracture bend rates were 2.62 mm±0.12 mm, 2.61 mm±0.15 mm and 2.81 mm±0.13 mm in Groups A, B and C, respectively. There was no significant difference between groups （PAB=0.7, PBc=0.448）. The ultimate anti-torsion torque values were 6.5

摘要：In the paper, we propose a surface wave suppression method in time-frequency domain based on the wavelet transform, considering the characteristic difference of polarization attributes, amplitude energy and apparent velocity between the effective signals and strong surface waves. First, we use the proposed method to obtain time-frequency spectra of seismic signals by using the wavelet transform and calculate the instantaneous polarizability at each point based on instantaneous polarization analysis. Then, we separate the surface wave area from the signal area based on the surface-wave apparent velocity and the average energy of the signal. Finally, we combine the polarizability, energy, and frequency characteristic to identify and suppress the signal noise. Model and field data are used to test the proposed filtering method.

摘要：Much like genomics, brain connectomics has rapidly become a core component of most national brain projects around the world. Beyond the ambitious aims of these projects, a fundamental challenge is the need for an efficient, robust, reliable and easy-to-use pipeline to mine such large neuroscience datasets. Here, we introduce a computational pipeline--namely the Connectome Compu- tation System （CCS）-for discovery science of human brain connectomes at the macroscale with multimodal magnetic resonance imaging technologies. The CCS is designed with a three-level hierarchical structure that includes data cleaning and preprocessing, individual connectome mapping andconnectome mining, and knowledge discovery. Several functional modules are embedded into this hierarchy to implement quality control procedures, reliability analysis and connectome visualization. We demonstrate the utility of the CCS based upon a publicly available dataset, the NKI- Rockland Sample, to delineate the normative trajectories of well-known large-scale neural networks across the natural life span （6-85 years of age）. The CCS has been made freely available to the public via GitHub （https：//***/ zuoxinian/CCS） and our laboratory＇s Web site （http：//lfcd. ***/***） to facilitate progress in discovery science in the field of human brain connectomics.

摘要：Engineering the electronic properties of catalysts to target intermediate adsorption energy as well as harvest high selectivity represents a promising strategy to design advanced electrocatalysts for efficient CO_(2) ***,a synergistical tuning on the electronic structure of the Cd Se nanorods is proposed for boosting electrochemical reduction of CO_(2) .The synergy of Ag doping coupled with Se vacancies tuned the electronic structure of the CdSe nanorods,which shows the metalloid characterization and thereby the accelerated electron transfer of CO_(2) *** synchrotron radiation Fourier transform infrared spectroscopy and theoretical simulation revealed that the Ag doping and Se vacancies accelerated the CO_(2) activation process and lowered the energy barrier for the conversion from CO_(2) to;COOH;as a result,the performance of CO_(2) electroreduction was *** as-obtained metalloid Ag-doped CdSe nanorods exhibited a 2.7-fold increment in current density and 1.9-fold Faradaic efficiency of CO compared with the pristine CdSe nanorod.

摘要：In this work,we carried out both theoretical calculation and experimental studies to reveal the contribution of hollow geometry to the light utilization efficiency of the TiO_2 photocatalysts in diluted aqueous *** is found that the single or multi-shelled hollow structures do not induce significant multiple reflections within the shells as widely believed in previous reports,and therefore the geometric factor has minimal contribution to the improvement of the light utilization efficiency of the *** design TiO_2 photocatalysts with higher activity,it is more appropriate to focus on the improvement of the crystallinity,diffusion,surface area,and dispersity of the catalysts,rather than their geometric shapes.

摘要：The effects of yttrium(Y)and yttrium+calcium(Y+Ca)additions on the electrochemical properties and discharge performance of the as-extruded Mg−8Al−0.5Zn−0.2Mn(AZ80)anodes for Mg−air batteries were *** results show that the addition of 0.2 wt.%Y increased the corrosion resistance and discharge activity of AZ80 *** was attributed to the fine and sphericalβ-Mg_17)Al_(12) phases dispersing evenly in AZ80+0.2Y alloy,which suppressed the localized corrosion and severe“chunk effect”,and facilitated the rapid activation ofα-*** addition of 0.2 wt.%Y and 0.15 wt.%Ca generated grain refinement and a reduction of theβ-Mg_17)Al_(12) phase,resulting in a further enhancement in discharge ***,the incorporation of Ca in Mg_17)Al_(12) and Al_(2)Y compounds compromised the corrosion resistance and anodic efficiency of AZ80+0.2Y+0.15Ca ***,AZ80+0.2Y anode exhibited excellent overall discharge performance,with the peak discharge capacity and anodic efficiency of 1525 mA·h·g^(−1) and 67%at 80 mA/cm^(2),13%and 14%higher than those of AZ80 anode,respectively.

摘要：Heterostructured photocatalysts provide an effective way to achieve enhanced photocatalytic performances through efficient charge *** both wide-and narrow-band-gap photocatalysts have been widely investigated,the charge separation and transfer mechanism at the contacting interface of the two has not been fully ***,a novel SrTiO3/BiOI(STB)heterostructured photocatalyst was successfully fabricated by using a facile *** heterostructure in the photocatalyst extends the photoabsorption to the visible light range,and thus,high photocatalytic NO removal performance can be achieved under visible light irradiation.A combination of experimental and theoretical evidences indicated that the photogenerated electrons from the BiOI semiconductor can directly transfer to the SrTiO3 surface through a preformed electron delivery *** electron transfer was expected between the SrTiO3 and BiOI surfaces under light irradiation,and leads to efficient ROS generation and thus a high NO conversion ***,in situ diffused reflectance infrared Fourier transform spectroscopy revealed that STB can better inhibit the accumulation of the toxic intermediate NO2 and catalyze the NO oxidation more *** work presents a new insight into the mechanism of the interfacial charge separation in heterostructures and provides a simple strategy to promote the photocatalytic technology for efficient and safe air purification.

摘要：This paper presents a two-wheeled differential spherical mobile robot in view of the problems that the motion of spherical robot is difficult to control and the sensor is limited by the spherical *** robot is simple in structure,flexible in motion and easy to *** kinematics and dynamics model of spherical mobile robot is established according to the structure of spherical mobile *** the basis of the adaptive neural sliding mode control,the trajectory tracking controller of the system is *** the simulation of the s-trajectory and circular trajectory tracking control of the spherical mobile robot,it is concluded that the spherical mobile robot is flexible in motion and easy to *** addition,the simulation results show that the adaptive neural sliding mode control can effectively track the trajectory of the spherical *** adaptive control eliminates the influence of unknown parameters and disturbances,and avoids the jitter of left and right wheels during the torque output.

摘要：Forward modeling is the basis of inversion imaging and quantitative interpretation for DC resistivity ***,a numerical model of the DC resistivity method must be finely divided to obtain a highly accurate solution under complex conditions,resulting in a long calculation time and large ***,we propose a 3D numerical simulation method in a mixed space-wavenumber domain to overcome this *** partial differential equation about abnormal potential is transformed into many independent ordinary differential equations with different wavenumbers using a 2D Fourier transform along the x axis and y axis *** this way,a large-scale 3D numerical simulation problem is decomposed into several 1D numerical simulation problems,which significantly reduces the computational and storage *** addition,these ordinary 1D differential equations with different wavenumbers are independent of each other and high parallelelism of the *** are solved using a finite-element algorithm combined with a chasing method,and the obtained solution is modified using a contraction *** this method,the vertical direction is reserved as the spatial domain,then grid size can be determined flexibly based on the underground current density distribution,which considers the solution accuracy and calculation *** addition,for the first time,we use the contraction operator in the integral equation method to iterate the *** algorithm takes advantage of the high efficiency of the standard Fourier transform and chasing method,as well as the fast convergence of the contraction *** verified the accuracy of the algorithm and the convergence of the contraction *** with a volume integral method and goal-oriented adaptive finite-element method,the proposed algorithm has lower memory requirements and high computational efficiency,making it suitable for calculating a model with large-scale ***,differ