摘要：肿瘤微环境中的血管生成是肿瘤细胞对放化疗不敏感的主要原因.在本项研究中,我们设计了一种微环境响应型的纳米颗粒,利用ZrMOF的分解产物抑制肿瘤细胞中的PI3K/AKT/mTOR/VEGF通路,从而克服A549R细胞对顺铂的耐药性.我们将顺铂包裹在Zr-MOF中,并在表面修饰BSA,形成了CDDP@Zr-MOF-BSA纳米复合体,该复合体在肿瘤微环境中有良好的响应性,有助于在肿瘤区域蓄积,展现出卓越的血管生成抑制能力,并能明显降低药物的外排.此外,CDDP@Zr-MOFBSA能显著抑制多药耐药相关蛋白1(MRP1)的表达,从而逆转A549R细胞的耐药性.总体而言,CDDP@Zr-MOF-BSA对A549R有细胞毒性,能抑制肿瘤微环境中的血管生成,最终有效提高顺铂耐药肿瘤的治疗效果.CDDP@Zr-MOF-BSA为放化疗治疗耐药肿瘤提供了一种多功能协同的方法.

摘要：Due to their special anatomical and physiological features,central nervous system diseases still presented challenges,despite the fact that some advances have been made in early diagnosis and precision *** of the complexities in treating tumors is the tumor microenvironment,which includes mesenchymal stem cells(MSCs)that exhibit tumor tropism and can be used for cell ***,whether MSCs promote or suppress gliomas is still unclear,especially in glioma *** this study,a coaxial microfiber was designed to mimic the tumor microenvironment and to reveal the effect of MSCs on glioma *** fiber shell was composed of MSCs and alginate,and the core was filled with U87 MG(glioblastoma)cells and gelatin *** Shell-MSC/Core-U87 MG microenvironment improved the proliferation,survival,invasion,metastasis,and drug resistance of glioma cells,while simultaneously maintaining the stemness of glioma *** summary,coaxial extrusion bioprinted Shell-MSC/Core-U87 MG microfiber is an ideal platform for tumor and stromal cell coculture to observe tumor biological behavior in vitro.

摘要：Recurrent oral ulcer is a painful oral mucosal disorder that affects 20%of the world’s *** lack of a radical cure due to its unknown underlying cause calls for innovative symptomatic *** work reports a hyaluronic acid-based dissolvablemicroneedle patch(ROUMNpatch,short for recurrent oral ulcer microneedle)loaded with dexamethasone acetate,vitamin C and tetracaine hydrochloride for the treatment of recurrent oral *** ROUMN patch shows enhancement in both the anti-inflammatory effect elicited by dexamethasone and the pro-proliferation effect of vitamin *** vitro experiments show that ROUMN has a higher efficiency in suppressing lipopolysaccharide(LPS)-induced interleukin-6(IL-6)expression than dexamethasone *** proliferation and migrationwere also significantly promoted byROUMNcompared to vitamin C *** healing-promoting effect of ROUMN was also verified in vivo using an acetic acid-cauterized oral ulcer model in *** as a treatment accelerated the healing process of oral ulcers,shortening the total healing time to 5 days compared with the 7 days required by treatment using watermelon frost,a commonly used over-the-counter(OTC)drug for oral *** rapid dissolution of the hyaluronic acid-based microneedles and the superior healing-promoting effect of the drug combination could lead to a broad application prospect of the ROUMN patch in the treatment of recurrent oral ulcers.

摘要：目的信息技术及相关产品在老年人的用药安全管理中具有广阔的市场前景。研究旨在探讨老年慢性病患者对自主研发的智能药物管理系统的需求,了解用户体验,为开发和完善适老化数字产品提供参考。方法通过横断面调查了解老年人对自主研发的智能药物管理系统的技术需求,并基于Kano模型对数据进行分析。于2020年7—10月采用基于Kano模型设计的问卷,对354例使用该智能药物管理系统的老年慢性病患者进行需求调查。结果调查结果显示,大字体、简化操作流程、定时用药提醒、硬件性能可靠为必备型属性;在微信上查询药品说明书、提高系统灵敏性、提高电池续航能力为魅力型属性;将疾病信息同步到微信为期望型属性;终端显示屏简洁美观、自动拦截广告、提供用户隐私保护、仅绑定用户可查看个人医疗信息、记录所服用的全部药品、通过微信订购药品为无差异型属性。具备相应功能时满意度值为0.24-0.69,未提供相应功能时不满意度值为0.06-0.94o结论老年人对信息技术存在个性化需求,满足其技术需求和倾向有助于提高老人对移动医疗健康技术的满意度和接受度,帮助老人迈过数字鸿沟。本研究基于需求分析结果,提出了智能药物管理系统的优化方案,为技术人员开发适老化智能产品及护士实施以老年人为中心的口服药智能化管理提供了参考。

摘要：Camelid single-domain antibody fragments(nanobodies)are an emerging force in therapeutic biopharmaceuticals and clinical diagnostic reagents in recent *** all nanobodies available to date have been obtained by animal immunization,a bottleneck restricting the large-scale application of *** this study,we developed three kinds of gene designatedregion pan-editing(GDP)technologies to introduce multiple mutations in complementarity-determining regions(CDRs)of nanobodies in *** the integration of G-quadruplex fragments in CDRs,which induces the spontaneous multiple mutations in CDRs;however,these mutant sequences are highly similar,resulting in a lack of sequences diversity in the *** also used CDR-targeting traditional gRNA-guided base-editors,which effectively diversify the *** most importantly,we developed the self-assembling gRNAs,which are generated by reprogrammed tracrRNA hijacking of endogenous mRNAs as *** base-editors guided by self-assembling gRNAs,we can realize the iteratively diversify the *** we believe the last GDP technology is highly promising in immunization-free nanobody library construction,and the full development of this novel nanobody discovery platform can realize the synthetic evolution of nanobodies in vitro.

摘要：目的本研究旨在确定影响老年人视力障碍的危险因素,建立和评价视力障碍风险预测模型。方法采用非匹配病例对照设计研究,2020年6一12月,从辽宁省某教学医院的眼科门诊和体检中心招募了586名研究参与者(411名被纳人到建模组,175名被纳人内部验证组)。视力障碍定义为最佳矫正视力<6/18(世界卫生组织定义)。调查了患者的基本情况和可能的预测因素,包括人口统计学资料、疾病和药物使用情况及生活方式。利用二元logistic回归分析建立视力障碍风险预测模型。以受试者工作特征曲线下面积(area under curve,AUC)评价预测模型的有效性。结果老年人视力障碍的6个独立影响因素为年龄、血管收缩压、体力活动评分、糖尿病、自我报告的眼部疾病史和文化程度。建立了老年人视力障碍风险预测模型,在建模组和内部验证组均显示出较强的预测能力,AUC分别为0.87(95%CI 0.83~0.90)和0.81(95%CI 0.74~0.88)。结论老年人视力障碍风险预测模型具有较高的预测能力。识别有视力障碍风险的老年人,有助于医护人员制订适当、有针对性的早期教育和干预计划,以预防和延缓老年人视力障碍的发生和发展,预防因视力障碍造成的伤害。

摘要：目的旨在调查老年缺血性脑卒中住院患者口腔健康现状并分析影响因素,为制订该人群口腔健康管理策略提供参考依据。方法采用横断面调查,于2022年1一6月选取重庆市2所三级甲等医院的350例老年缺血性脑卒中患者,采用巴氏指数(Barthel Index,BI)、进食评估工具(Eating Assessment Tool-10,EAT-10)和口腔健康评估量表(Oral Health Assessment Tool,OHAT)分别调查研究对象的自理程度、吞咽功能和口腔健康状况,采用自行设计的调查表调查人口统计学、疾病相关信息及口腔健康行为。采用二元logistic回归分析进行相关影响因素分析。结果共纳人346例老年缺血性脑卒中患者,其中男性199例,女性147例。0HAT总得分中位数为5分(总得分范围0~16分)。龋齿(91.7%,278/303)和口腔清洁不良(92.2%,319/346)为该人群主要的口腔健康问题。二元logistic回归分析结果显示,性别、血脂水平、脑卒中发生次数和严重程度、口腔健康行为、自理程度为该人群口腔健康的影响因素(P<0.05)。结论医务人员应加强老年缺血性脑卒中患者口腔健康评估,并针对高危人群的特点实施个性化的健康教育和管理措施,促进其口腔健康。

摘要：AIM: To describe the design and preliminary results of the hospital based epidemiological study for diabetic retinopathy (HBESDR), an ongoing epidemiological study to estimate the prevalence of diabetic retinopathy (DR) and to elucidate the clinical, anthropometric, biochemical and any other risk factors associated with diabetic retinopathy. METHODS: Totally 2000 diabetes will be recruited from the Diabetes eye clinic in the first Affiliated hospital of china medical university. All subjects underwent blood sugar estimation and Oral Glucose Tolerance Test to diagnose diabetes. All diabetes would undergo complete questionnaire, a comprehensive eye examination. Blood and urine would be collected for biochemical investigations. All fundus photographs for any DR will be graded. Participants who need treatment will be sent to the ophthalmic clinic and follow-up interval program for all subjects will be suggested. A computerized database is created for the records. RESULTS: To date, 1174 diabetes have been recruited, there were 350 (29.81%) DR in all diabetes, most of them were with mild non-proliferative diabetic retinopathy (NPDR) (139, 39.71%); 71 (20.29%) moderate NPDR, 66 (18.86%) severe NPDR, 74 (21.14%) proliferative diabetic retinopathy (PDR). Females, longer duration of diabetes, family history of diabetes and hypertension had a statistically significant increase in risk of any DR. CONCLUSION: The study is expected to provide an estimate of the overall prevalence of DR and the prevalence with different duration of diabetes and also a better understanding of the risk factors associated with DR.

摘要：Cardiovascular diseases are the leading cause of morbidity and mortality throughout the world underlining the importance of efficient treatments including disease modeling and drug discovery by cardiac tissue ***,the predictive power of these applications is currently limited by the immature state of the ***,we developed gelatin hydrogels chemically crosslinked by genipin,a biocompatible crosslinker,as cell culture *** rat cardiomyocytes appear synchronous beatingwithin 2 days after seeding on ***,we applied the electrical stimulation as a conditioning treatment to promote the maturation of cardiomyocytes cultured on the *** results show that electrical stimulation improves the organization of sarcomeres,establishment of gap junctions,calcium-handling capacity and propagation of pacing signals,thereby,increase the beating velocity of cardiomyocytes and responsiveness to external *** above system can be applied in promoting physiological function maturation of engineered cardiac tissues,exhibiting promising applications in cardiac tissue engineering and drug screening.

摘要：Abstract Meniscus injury is a common disease in *** it was not treated in time,it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the *** present,meniscectomy and meniscus suture are widely used in the treatment for meniscus ***,It is not ideal for poor self-healing ability of *** recruitment of endogenous stem cells is an attractive option for wounded meniscus *** reduced high-mobility group box 1 protein(HMGB1)can accelerate the regeneration of multiple tissues by endogenous stem cell activation,migration and ***(KGN)has shown to induce the chondrogenesis of the stem ***,no study has explored such effects of HMGB1 and KGN in wounded meniscus ***,in order to improve the regeneration of meniscus,we intend to use a novel bioactive microsphere which was developed by combining fully reduced high mobility group box 1(frHMGB1)and kartogenin(KGN)with alginate gel which slowly release high concentrations of HMGB1 and KGN to activate rat bone marrow stem cells(BMSCs)and promote cell *** results showed that this HMGB1–KGN microsphere released and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 *** vitro experimental results showed that the HMGB1–KGN microsphere can promote cell proliferation via recruiting rat bone marrow stem cells(BMSCs)and activating the BMSCs from G_(0) to G_(Alert) stage as evidenced by cell migration testing and 5-bromo-2′-deoxyuridine(BrdU)incorporation *** vivo results indicated that this HMGB-KGN microsphere can recruit GFP-labeled BMSCs from tail vein to wounded meniscus and induce these GFP-labeled BMSCs to differentiate into *** results demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus healing in vivo.