摘要：To investigate the feasibility of implanting the biocomposite of calcium phosphate cement(CPC)/polylactic acid-polyglycolic acid(PLGA) into animals for bone defects repairing,the biocomposite of CPC/PLGA was prepared and its setting time,compressive strength,elastic modulus,pH values,phase composition of the samples,degradability and biocompatibility in vitro were *** above-mentioned composite implanted with bone marrow stromal cells was used to repair defects of the radius in *** was histomorphologically observed by using an *** results show that compared with the CPC,the physical and chemical properties of CPC/PLGA composite have some differences in which CPC/PLGA composite has better biological *** CPC/PLGA composite combined with seed cells is superior to the control in terms of the amount of new bones formed after CPC/PLGA composite is implanted into the rabbits,as well as the speed of repairing bone *** results suggest that the constructed CPC/PLGA composite basically meets the requirements of tissue engineering scaffold materials and that the CPC/PLGA composite implanted with bone marrow stromal cells may be a new artificial bone material for repairing bone defects because it can promote the growth of bone tissues.

摘要：Microwave assisted-semi bionic extraction (MASBE) process for lignans from Fructus Forsythiae was studied. The influences of solvent pH value, microwave power, dosage of solvent and irradiation time were investigated. Optimum extracting parameters were determined by orthogonal experiments as follows: pH value of solvent at first extraction is 5.5-6.0, that at the second extraction is 7-8; microwave power is 700 W; mass ratio of Fructus Forsythiae to water is 1-12; irradiation time is 10 min, and extracting times is two. Under these optimal conditions, the yield of lignans reaches 0.364%. Compared with the conventional extraction methods, the MASBE process has the advantages of high extraction rate, high extraction selectivity.

摘要：腔静脉滤器是为预防深静脉血栓脱落导致致命性肺栓塞而设计的装置,在滤器回收过程中常出现不同程度的技术困难,需借助辅助器械和静脉通路。建立有效的预测模型提前预测滤器回收困难程度有助于术前规划、沟通和术中高效配合。本文回顾性分析2011年1月至2020年12月在中南大学湘雅三医院进行的477例腔静脉滤器回收病例,其中顺利回收344例,困难性回收137例(包括35例回收失败)。运用单因素与多因素logistic回归分析筛选困难性回收的相关风险因素,再构建Nomogram预测模型并用ROC曲线验证模型的准确性,其中2011年1月至2017年12月病例资料为训练队列,2018年1月至2020年12月资料为验证队列。单因素分析结果显示滤器是否贴壁、滤器是否穿透腔静脉、滤器与血管长轴之间夹角、实际使用时间与建议置入时间比值、是否规律抗凝是腔静脉滤器困难性回收的风险因素,在此基础上进一步使用logistic多因素回归分析显示,滤器是否贴壁、滤器是否穿透腔静脉、是否规律抗凝是预测腔静脉滤器困难性回收的独立风险因素。将上述三种风险进一步构建Nomogram预测模型,内部验证评价AUC值为0.819,外部验证评价AUC值为0.817;内部验证和外部验证的校准曲线均提示模型能有效预测困难性回收风险。构建腔静脉滤器困难性回收Nomogram图预测模型的纳入变量在术前易于获得,模型具有较好的预测效果和较低的泛化误差,可为血管介入医生在腔静脉滤器回收治疗决策提供重要依据。

摘要：Purpose Skin pigmentation disorders,such as vitiligo and melasma,are difficult to diagnose in the early stages,but abnor-mal tyrosinase levels and tyrosinase activity are potential *** resorufin-based fluorescence probes(RBFPs)have been designed to detect tyrosinase in tumors,but they have not been used in skin pigmentation *** this study,one of these RBFPs(synthesized by resorufin salt coupled with 3-(bromomethyl)phenol)was evaluated *** The RBFP was tested in different kinds of mouse and human skin cells,as well as in in vivo models,including zebrafish,guinea pigs,and Sprague-Dawley *** addition,small interfering RNAs(siRNAs),kojic acid,and 1-phenyl-2-thiourea(PTU)were used to inhibit tyrosinase levels or tyrosinase *** This probe successfully detected tyrosinase and emitted red fluorescence in melanoma cells and *** was also observable in zebrafish and on the skin of guinea pigs when using the *** mouse and human cells,the RBFP showed good selectivity to ***,in the case of decreased tyrosinase levels or activity caused by siRNAs,kojic acid,or PTU,the probe was sensitive to these ***,the RBFP showed no toxic effects at concentrations of<20μmol/L,both in vitro and in *** Our findings indicate the value and limitations of the RBFP in tyrosinase detection,but suggest the need for further improvement of fluorescent probes in the diagnosis of skin pigmentation disorders.

摘要：Objective:Sleep quality in kidney transplant recipients is closely associated with symptoms of fatigue and *** subjective assessment tools like the Pittsburgh Sleep Quality Index and the Richards-Campbell Sleep Questionnaire(RCSQ)are widely used to evaluate sleep quality,there is a lack of studies utilizing polysomnography for objective *** study aims to investigate the correlation between sleep quality,fatigue,and depression in kidney transplant recipients using both subjective and objective methods,providing scientific evidence for improving their quality of ***:The cross-sectional study conveniently sampled 50 kidney transplant recipients from a transplant center in a general hospital between August 2018 and March *** and objective sleep parameters were evaluated using the RCQS and polysomnography,*** Fatigue Severity Scale was used to assess fatigue,and the Hamilton Depression Scale was employed to measure depression ***:A lower proportion of rapid eye movement(REM)sleep was associated with increased ***,higher wake time percentages and poorer sleep quality were significantly correlated with greater depression ***:This study underscores the critical importance of effectively managing sleep quality in kidney transplant recipients and addressing their fatigue and depression *** findings lay a foundation for developing targeted nursing and therapeutic strategies.

摘要：Atrial fibrillation is an “invisible killer” of human health. It often induces high-risk diseases, such as myocardial infarction, stroke, and heart failure. Fortunately, atrial fibrillation can be diagnosed and treated early. Low-level vagus nerve stimulation(LL-VNS) is a promising therapeutic method for atrial fibrillation. However, some fundamental challenges still need to be overcome in terms of flexibility,miniaturization, and long-term service of bioelectric stimulation devices. Here, we designed a closedloop self-powered LL-VNS system that can monitor the patient’s pulse wave status in real time and conduct stimulation impulses automatically during the development of atrial fibrillation. The implant is a hybrid nanogenerator(H-NG), which is flexible, light weight, and simple, even without electronic circuits,components, and batteries. The maximum output of the H-NG was 14.8 V and 17.8 μA(peak to peak). In the in vivo effect verification study, the atrial fibrillation duration significantly decreased by 90% after LLVNS therapy, and myocardial fibrosis and atrial connexin levels were effectively improved. Notably, the anti-inflammatory effect triggered by mediating the NF-κB and AP-1 pathways in our therapeutic system is observed. Overall, this implantable bioelectronic device is expected to be used for self-powerability,intelligentization, portability for management, and therapy of chronic diseases.

摘要：Objective A classification and diagnosis model for psoriasis based on deep residual network is proposed in this *** using deep learning technology to classify and diagnose psoriasis can help reduce the burden of doctors,simplify the diagnosis and treatment process,and improve the quality of *** Firstly,data enhancement,image resizings,and TFRecord coding are used to preprocess the input of the model,and then a 34-layer deep residual network(ResNet-34)is constructed to extract the characteristics of ***,we used the Adam algorithm as the optimizer to train ResNet-34,used cross-entropy as the loss function of ResNet-34 in this study to measure the accuracy of the model,and obtained an optimized ResNet-34 model for psoriasis *** The experimental results based on k-fold cross validation show that the proposed model is superior to other diagnostic methods in terms of recall rate,F1-score and ROC *** The ResNet-34 model can achieve accurate diagnosis of psoriasis,and provide technical support for data analysis and intelligent diagnosis and treatment of psoriasis.