摘要：The third-generation(3G)biorefinery aims to use microbial cell factories or enzymatic systems to synthesize value-added chemicals from one-carbon(C1)sources,such as CO_(2),formate,and methanol,fueled by renewable energies like light and *** promising technology represents an important step toward sustainable development,which can help address some of the most pressing environmental challenges faced by modern ***,to establish processes competitive with the petroleum industry,it is crucial to determine the most viable pathways for C1 utilization and productivity and yield of the target *** this review,we discuss the progresses that have been made in constructing artificial biological systems for 3G biorefineries in the last 10 ***,we highlight the representative works on the engineering of artificial autotrophic microorganisms,tandem enzymatic systems,and chemobio hybrid systems for C1 *** also prospect the revolutionary impact of these developments on *** harnessing the power of 3G biorefinery,scientists are establishing a new frontier that could potentially revolutionize our approach to industrial production and pave the way for a more sustainable future.