摘要：Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and *** this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana *** the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of *** of this enzyme in the ntggpps1 mutant leads to decreased carotenoid *** the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence *** activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type ***,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to *** findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield *** results open the door for the structure-guided rational design of elite genes in higher plants。

摘要：A novel tobacco-curing system with a solar-assisted heat pump was *** proposed system has various advantages,such as reducing energy consumption and pollutant emissions and enhancing the stability of system *** thermal characteristics of the system under different climate conditions were analyzed,and the economic and environmental benefits of different tobacco-curing technologies were *** indicated that the dehydration rate,the mass flux of exhaust air,and the heat load of the novel tobacco-curing system in different months had similar changes with the baking time,and all reached the maximum in the later stage of color *** with the power saving rate of a heat pump tobacco-curing system,that of the novel system reached 25.9%-35.1%.The dry leaf curing cost of the novel system was only 0.86-1.06 yuan/kg,which can reduce the cost by more than 60%compared with traditional coal-burning tobacco-curing systems in *** with other parts of the tobacco leaf,the top leaf had the lowest dry leaf curing cost due to its highest mass of dry *** payback period and the annual CO_(2) emission reduction of the novel system were 3.0-3.7 a and 15586 kg,respectively.